Addgene

We narrowed to 17,136 results for: STO

Showing: 1341 - 1360 of 17136 results

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pJFRC177-10XUAS-FRT>-dSTOP-FRT>-myr::GFP

Plasmid

#32149

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

Insert

FRT> STOP STOP FRT>

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 23, 2011

Availability

Academic Institutions and Nonprofits only

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EK0307 CMV-TO-BFPmut-stop-3UTR(Bdnf) (FLP-IN)

Plasmid

#191145

Purpose

Expression of a synthetic construct with 3' UTR of murine Bdnf in the 3' UTR of an inactive BFP in mammalian cells.

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

3UTR(Bdnf)

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

CMV-TO

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pXL-BacII-CAG-loxP-STOP-loxP-Venus

Plasmid

#170520

Purpose

Plasmid containing piggyBac ITRs flanking CAG, a loxP-STOP-loxP cassette and Venus (also see doi: 10.7554/eLife.63907 )

Depositor

Joshua Sanes

Article

Yamagata et al Bio Protoc. 2021 Aug 5;11(15):e4105. doi

Insert

CAG-loxP-STOP-loxP-Venus

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-DIO-inv[roxSTOProx-EB3 mNeonGreen-bGH]

Plasmid

#196885

Purpose

Dual Cre/Dre-dependent expression of EB3-mNeon. Used as functional (labelling of microtubule plus ends) Cre-Dre cotransfection reporter

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

roxSTOProx-EB3 mNeonGreen-bGH (MAPRE3 Human)

Use

Cre/Lox; Dre/rox

Tags

Expression

Mammalian

Mutation

Promoter

Quimeric CAG

Available since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223 ERFlux (Bip-HaloTag7-mGFP-KDEL-STOP)

Plasmid

#215002

Purpose

Shuttling vector for an ERphagy reporter

Depositor

Wade Harper

Insert

KDEL

Use

Gateway

Tags

HaloTag(7)-mGFP

Expression

Mutation

Promoter

Available since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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pJFRC160-21XUAS-B3RT>-dSTOP-B3RT>-myr::RFP

Plasmid

#32139

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

Insert

B3RT> STOP STOP B3RT>

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Nov. 2, 2011

Availability

Academic Institutions and Nonprofits only

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pJFRC172-10XUAS-loxP>-dSTOP-loxP>-myr::GFP

Plasmid

#32145

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

Insert

loxP> STOP STOP loxP>

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 23, 2011

Availability

Academic Institutions and Nonprofits only

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p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMyc[open]

Plasmid

#71809

Purpose

Generating transgenic flies for FLP-inducible DamID

Depositor

Bas van Steensel

Article

Pindyurin et al Nucleic Acids Res. 2016 Jul 8;44(12):564

Insert

min.hsp70P-FRT-STOP#1-FRT-Dam-Myc[open]

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 2, 2016

Availability

Academic Institutions and Nonprofits only

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p255 pPr-CREM/Ins/CMV-STOP-luc

Plasmid

#8408

Purpose

Modified cre expressed from probasin, separated from the Cre-dependent CMV-STOP-luc cassette by insulators.

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

Insert

CREM/insulators/lox/luciferase

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

modified Cre

Promoter

Probasin, CMV

Available since

April 26, 2006

Availability

Academic Institutions and Nonprofits only
pLenti UBC LC3Flux (HaloTag7-mGFP-LC3-STOP)

Plasmid

#215014

Purpose

Express an autophagy (LC3) reporter in mammalian cells (untested)

Depositor

Wade Harper

Insert

MAP1LC3B (Map1lc3b Rat)

Use

Lentiviral

Tags

HaloTag(7)-mGFP

Expression

Mammalian

Mutation

Promoter

Available since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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aCat TL2 (1-697 mTFP1-TSmod-Venus STOP)

Plasmid

#101298

Purpose

FRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin.

Depositor

Alpha Yap

Article

Acharya et al Cell Rep. 2017 Mar 21;18(12):2854-2867.

Insert

alpha-Catenin

Use

Tags

Expression

Mammalian

Mutation

Contains amino acids 1-698 of alpha-Catenin

Promoter

Available since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCFJ2089 - [1-2] ENTR - Tag - SNAP(PATC, no_atg, no_stop)

Plasmid

#159874

Purpose

Three-fragment Gateway compatible protein tag for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Tag - SNAP(PATC, no_atg, no_stop)

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pJFRC168-21XUAS-RSRT>-dSTOP-RSRT>-myr::RFP

Plasmid

#32143

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

Insert

RSRT> STOP STOP RSRT>

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Nov. 2, 2011

Availability

Academic Institutions and Nonprofits only

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pOTTC1179 - pAAV (flox-stop) TH gRNA A EF1a eGFP

Plasmid

#113158

Purpose

An AAV vector that expresses a Cre-dependent guide RNA targeting rat TH and expresses EGFP reporter

Depositor

Brandon Harvey

Article

Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

Insert

gRNA for rat TH

Use

AAV, CRISPR, and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

mU6

Available since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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pJFRC176-10XUAS-rox>-dSTOP-rox>-myr::GFP

Plasmid

#32147

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

Insert

rox> STOP STOP rox>

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 23, 2011

Availability

Academic Institutions and Nonprofits only

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pJFRC156-21XUAS-B2RT>-dSTOP-B2RT>-myr::RFP

Plasmid

#32135

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

Insert

B2RT> STOP STOP B2RT>

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Nov. 2, 2011

Availability

Academic Institutions and Nonprofits only

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C514-E23: attB5r-RU5u-3xFLAG-nostop-attB1r

Plasmid

#162958

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal 3xFLAG-tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

triple FLAG epitope tag with upstream HTLV RU5 5' UTR

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMyc[closed]

Plasmid

#71810

Purpose

Generating transgenic flies for FLP-inducible DamID

Depositor

Bas van Steensel

Article

Pindyurin et al Nucleic Acids Res. 2016 Jul 8;44(12):564

Insert

min.hsp70P-FRT-STOP#1-FRT-Dam-Myc[closed]

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 2, 2016

Availability

Academic Institutions and Nonprofits only

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C514-E67: attB5r-RU5u-Strep2x2-nostop-attB1r

Plasmid

#162967

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal dual Strep-tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Dual Strep2 purification tag with upstream HTLV RU5 5' UTR

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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pBactin-DIO-inv[roxSTOProx-EB3-mNeonGreen-bGH]

Plasmid

#196887

Purpose

Dual Cre/Dre-dependent expression of EB3-mNeon. Used as functional (labelling of microtubule plus ends) Cre-Dre cotransfection reporter

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

roxSTOProx-EB3 mNeonGreen-bGH (MAPRE3 Human)

Use

Cre/Lox; Dre/rox

Tags

Expression

Mammalian

Mutation

Promoter

Chicken bactin (plus Chicken bactin intron)

Available since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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