We narrowed to 2,328 results for: dap

1. qTAG-C-Solo-mStayGold-PXN

   Plasmid

   #227316

   Purpose

   Donor template for mStayGold insertion into the C-terminus of the PXN locus. For focal adhesion visualization. To be co-transfected with sgRNA plasmid px330-PITCh-PXN (Addgene #227315)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   PXN Homology Arms flanking a mStayGold Tag (PXN Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pmiRGLO Dual-Luciferase-PIK3R1-3'UTR

   Plasmid

   #182252

   Purpose

   Human PIK3R1 3` UTR region containing 1x binding site for miR-150 cloned downstream of luciferase reporter gene

   Depositor

   Silvia Monticelli

   Article

   Chirichella et al PLoS Biol. 2022 Feb 10;20(2):e3001538. d

   Insert

   3' UTR region of PIK3R1 gene
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. qTAG-C-Solo-mScarlet-H2BC11

   Plasmid

   #207756

   Purpose

   Donor template for mScarlet insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   H2BC11 Homology Arms flanking a mScarlet Tag (H2BC11 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 16, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pCas4_1_2_VA

   Plasmid

   #186438

   Purpose

   Encodes Cas4, Cas1, Cas2, leader, repeat and one spacer from F. Novicida (type V-A)

   Depositor

   John van der Oost

   Article

   Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

   Insert

   Cas4, Cas1 and Cas2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX-4T-1-β4 hinge-ear

   Plasmid

   #196692

   Purpose

   Expresses GST-AP-4 β4 hinge-ear (residues 534-739) in bacteria

   Depositor

   Juan Bonifacino

   Article

   Mattera et al J Biol Chem. 2015 Dec 25;290(52):30736-4

   Insert

   AP-4 β4 hinge-ear (534-739) (AP4B1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-AMPKsub-YPet-NES

   Plasmid

   #84635

   Purpose

   Encodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

   Depositor

   Jin Zhang

   Article

   Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

   Insert

   AMPKsub-YPet-NES
   

   Use

   Tags

   6xHis, Nuclear export signal (NES), T7 tag (gene …

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRS315e_pGal-dCas9-SH3-pGal-PmCDA1-SHL

   Plasmid

   #79614

   Purpose

   Expresses dCas9-SH3 and PmCDA1-SHL in yeast cells

   Depositor

   Akihiko Kondo

   Article

   Nishida et al Science. 2016 Aug 4. pii: aaf8729.

   Inserts

   SpCas9
   PmCDA1
   

   Use

   Tags

   SH3 domain and SHL

   Expression

   Yeast

   Mutation

   D10A and H840A for nuclease deficient Cas9

   Promoter

   pGal1 and pGal10

   Available since

   July 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX4T1-GST-LC3B

   Plasmid

   #216836

   Purpose

   For expression in E. coli of GST-LC3B (Gly exposed) (Internal ref. SMC1463)

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al bioRxiv September 25, 202

   Insert

   LC3B (Gly exposed) (MAP1LC3B Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   TAC promoter

   Available since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-mCherry-LKB1(KD)

   Plasmid

   #84640

   Purpose

   mCherry-tagged, LKB1(K78I); kinase-dead mutant

   Depositor

   Jin Zhang

   Article

   Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

   Insert

   mCherry-LBK1(K78I) (STK11 Human, Synthetic)
   

   Use

   Tags

   6xHis, T7 tag (gene 10 leader), Xpress (TM) tag, …

   Expression

   Mammalian

   Mutation

   Lysine 78 mutated to Isoleucine; kinase dead

   Promoter

   CMV

   Available since

   May 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. Intersectin I SH3 A domain (human)

    Plasmid

    #47413

    Depositor

    Peter McPherson

    Article

    Yamabhai et al J Biol Chem. 1998 Nov 20;273(47):31401-7

    Insert

    Intersectin SH3 domain A (ITSN1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    only SH3 domain A

    Promoter

    tac

    Available since

    July 29, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pGEX-6P-2_dCrk

    Plasmid

    #131146

    Purpose

    Expresses GST::dCrk (based on crk isoform RA).

    Depositor

    Mark Peifer

    Article

    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

    Insert

    Crk (Crk Fly)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-GST-hFIP200(1363-1370Mut)-MBP

    Plasmid

    #190038

    Purpose

    Expression of FIP200 mutant

    Depositor

    James Hurley

    Article

    Shi et al Elife. 2020 Aug 10;9. pii: 59099. doi: 1

    Insert

    FIP200 (RB1CC1 Human)
    

    Use

    Tags

    GST and MBP

    Expression

    Mammalian

    Mutation

    1363 RDKDLIES 1370 to GSSGGSSG

    Promoter

    CMV

    Available since

    Nov. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1_pCMV-nCas-PmCDA1 pH1-gRNA(HPRT)

    Plasmid

    #79619

    Purpose

    Expresses nCas9-PmCDA1 and gRNA(HPRT) in mammalian cells

    Depositor

    Akihiko Kondo

    Article

    Nishida et al Science. 2016 Aug 4. pii: aaf8729.

    Insert

    SpCas9
    

    Use

    Tags

    3xFlag-PmCDA1

    Expression

    Mammalian

    Mutation

    D10A for nickase Cas9

    Promoter

    pCMV

    Available since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX330-PITCh-H3C2

    Plasmid

    #207780

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of H3C2 for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of H3C2 (H3C2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1_pCMV-dCas-PmCDA1 pH1-gRNA(HPRT)

    Plasmid

    #79621

    Purpose

    Expresses dCas9-PmCDA1 and gRNA(HPRT) in mammalian cells

    Depositor

    Akihiko Kondo

    Article

    Nishida et al Science. 2016 Aug 4. pii: aaf8729.

    Insert

    SpCas9
    

    Use

    Tags

    PmCDA1

    Expression

    Mammalian

    Mutation

    D10A and H840A for nuclease deficient Cas9

    Promoter

    pCMV

    Available since

    Sept. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. Myc-Cdh1

    Plasmid

    #28127

    Depositor

    Pumin Zhang

    Article

    Li et al Nat Cell Biol. 2008 Sep . 10(9):1083-9.

    Insert

    fzr1 (FZR1 Human)
    

    Use

    Mouse Targeting and Retroviral

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 15, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pMRX-IPU-FLAG-NEK9T210A

    Plasmid

    #168270

    Purpose

    Expresses kinase-dead NEK9 mutant in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Yamamoto et al Nat Commun. 2021 Jun 2;12(1):3292. doi:

    Insert

    NEK9 (NEK9 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed threonine 210 to alanine

    Promoter

    Available since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX6P1 UBXN11

    Plasmid

    #169017

    Purpose

    Expresses GST-UBXN11 (human) in bacteria

    Depositor

    Hemmo Meyer

    Article

    Kracht et al J Mol Biol. 2020 Nov 20;432(23):6061-607

    Insert

    UBXN11 (UBXN11 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Intersectin I SH3 B domain (human)

    Plasmid

    #47414

    Depositor

    Peter McPherson

    Article

    Yamabhai et al J Biol Chem. 1998 Nov 20;273(47):31401-7

    Insert

    Intersectin SH3 domain B (ITSN1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    only SH3 domain B

    Promoter

    tac

    Available since

    July 29, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pMT2-MEKK3-K392A

    Plasmid

    #21585

    Depositor

    John Kyriakis

    Article

    Chadee et al Mol Cell Biol. 2002 Feb . 22(3):737-49.

    Insert

    mitogen-activated protein kinase kinase kinase 3 (MAP3K3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Lysine 392 to Arginine

    Promoter

    Available since

    Nov. 24, 2009

    Availability

    Academic Institutions and Nonprofits only