We narrowed to 1,480 results for: halo

1. M26I DJ-1 TEV site pET15b

   Plasmid

   #60685

   Purpose

   Recombinant protein expression of Park7

   Depositor

   Mark Wilson

   Article

   Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

   Insert

   M26I DJ-1 (PARK7 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   M26I and TEV site was inserted (see comments belo…

   Promoter

   T7

   Available since

   Oct. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

2. M26L DJ-1 TEV site pET15b

   Plasmid

   #60686

   Purpose

   Recombinant protein expression of Park7

   Depositor

   Mark Wilson

   Article

   Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

   Insert

   M26L DJ-1 (PARK7 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   M26L and TEV site was inserted (see comments belo…

   Promoter

   T7

   Available since

   Oct. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pET15b-NHis6-NocL

   Plasmid

   #240398

   Purpose

   expresses N-His tagged NocL (fatty acid activating ligase) from Nodularia sp. 06071

   Depositor

   Emily Balskus

   Article

   Wang et al Biochemistry. 2025 May 20;64(10):2173-21

   Insert

   nocL(fatty acyl-AMP ligase)
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. MZB160

   Plasmid

   #217841

   Purpose

   Bacterial expression of 10xHis-SUMO (Smt3) fusion to HRV-3C cleavable Halotagged Arf (L8K+Q71L) for pulldown assays

   Depositor

   Richard Baker

   Article

   Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

   Insert

   Arf1 (ARF1 Human)
   

   Use

   Tags

   10x His, HaloTag, and SUMO (SMT3)

   Expression

   Bacterial

   Mutation

   L8K, Q71L

   Promoter

   Available since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. MZB224

   Plasmid

   #226998

   Purpose

   Bacterial expression of 10xHis-SUMO (Smt3) fusion to HRV-3C cleavable Halotagged Arf (T31N) for pulldown assays

   Depositor

   Richard Baker

   Article

   Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

   Insert

   Arf1 (ARF1 Human)
   

   Use

   Tags

   10x His, HaloTag, and SUMO (SMT3)

   Expression

   Bacterial

   Mutation

   T31N

   Promoter

   T7

   Available since

   Jan. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEMS2371

   Plasmid

   #225637

   Purpose

   High Copy number plasmid containing Ple388 (PITX3 MiniPromoter) insert

   Depositor

   Elizabeth Simpson

   Article

   Fornes et al Nucleic Acids Res. 2023 Jul 5;51(W1):W37

   Insert

   Ple388 (PITX3 Human)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. px552-sg-gria1-HT

   Plasmid

   #187652

   Purpose

   Contains HaloTag to be inserted into the NTD of Gria1 (via HITI), single guide RNA to target Cas9 to Gria1 under control of the U6 promoter, and miRFP670 under control of human synapsin promoter.

   Depositor

   Erin O'Shea

   Article

   Wong et al Elife. 2024 Aug 15;13:e80622. doi: 10.75

   Inserts

   HaloTag donor sequence
   miRFP670
   

   Use

   AAV and CRISPR

   Tags

   N/A

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFsynW SYT1-PGM reporter

   Plasmid

   #197278

   Purpose

   Encodes for viral expression of Synaptotagmin 1 (SYT1) for use in the RUSH system; visualized with HaloTag. Reporter only (no hook). SYT1 is mutated to disrupt palmitoylation and glycosylation.

   Depositor

   Edwin Chapman

   Article

   Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

   Insert

   SYT1 and HaloTag (Syt1 Rat)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   T15A, T16A, N24Q, C74A, C75A, C77A, C79A, and C82A

   Promoter

   hSyn (human synapsin I promoter)

   Available since

   July 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET51b-His-TEV-SNAP-tag_dead_mt

   Plasmid

   #167270

   Purpose

   Overexpression of the dead variant (C145A) of SNAP-tag in E. coli

   Depositor

   Kai Johnsson

   Article

   Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

   Insert

   SNAP-tag
   

   Use

   Tags

   His x10

   Expression

   Bacterial

   Mutation

   C145A

   Promoter

   T7

   Available since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET51b-His-TEV-SNAP-tag_fast_dead_mt

    Plasmid

    #167272

    Purpose

    Overexpression of the fast (E30R) dead variant (C145A) of SNAP-tag in E. coli

    Depositor

    Kai Johnsson

    Article

    Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

    Insert

    SNAPf-tag
    

    Use

    Tags

    His x10

    Expression

    Bacterial

    Mutation

    E30R-C145A

    Promoter

    T7

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET14b-dabAB2 D353A

    Plasmid

    #133016

    Purpose

    pET14b carrying the dabA2 gene (Uniprot: D0KWS7) with a D353A mutation fused to a c-terminal strep tag and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tag

    Depositor

    David Savage

    Article

    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

    Inserts

    dabB2
    dabA2
    

    Use

    Tags

    6xHis, StrepII tag, and sfGFP

    Expression

    Bacterial

    Mutation

    D353A

    Promoter

    t7

    Available since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET14b-dabAB2 C351A

    Plasmid

    #133014

    Purpose

    pET14b carrying the dabA2 gene (Uniprot: D0KWS7)  with a C351A mutation fused to a c-terminal strep tag and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tag

    Depositor

    David Savage

    Article

    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

    Inserts

    dabB2
    dabA2
    

    Use

    Tags

    6xHis, StrepII tag, and sfGFP

    Expression

    Bacterial

    Mutation

    C351A

    Promoter

    t7

    Available since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1/CIP2A(1-905) WT V5 His

    Plasmid

    #119287

    Purpose

    CIP2A WT V5 overexpression

    Depositor

    Jukka Westermarck

    Article

    Wang et al EMBO Rep. 2017 Mar;18(3):437-450. doi: 1

    Insert

    KIAA1524 (CIP2A Human)
    

    Use

    Tags

    V5 His

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Jan. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHTN-LC3B

    Plasmid

    #219440

    Purpose

    Expresses HaloTag-LC3B, an autophagosome marker

    Depositor

    Erika Holzbaur

    Article

    Tudorica et al J Cell Biol. 2024 Jul 1;223(7):e20230901

    Insert

    LC3B (Map1lc3b Rat)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-SynI-CreOn-FlpOff-mNaChBac-P2A-EGFP

    Plasmid

    #176280

    Purpose

    Viral vector for co-expression of NaChBac and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.

    Depositor

    Martin Riccomagno

    Article

    Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    mNaChBac-P2A-EGFP
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human Synapsin I

    Available since

    Jan. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pINDUCER13 (miR-LUP)

    Plasmid

    #46936

    Purpose

    Inducible lentiviral gene silencing vector. Insert PheSGly294, (XhoI to EcoRI; 1.4kb), can be replaced w mir30 based hairpin of interest

    Depositor

    Thomas Westbrook

    Article

    Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

    Insert

    PheS Gly294
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-Syn-ArchT-GFP

    Plasmid

    #63141

    Purpose

    AAV-mediated expression of ArchT-GFP under the Syn promoter. Using hGHpA signal.

    Depositor

    Edward Boyden

    Article

    Han et al Front Syst Neurosci. 2011;5:18. Epub 201

    Insert

    ArchT-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin promoter

    Available since

    May 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-CaMKIIa-eArchT 3.0-EYFP

    Plasmid

    #35513

    Purpose

    Lentiviral expression of CaMKIIa-driven ArchT 3.0 for optical inhibition.

    Depositor

    Karl Deisseroth

    Article

    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

    Insert

    eArchT 3.0-EYFP
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Enhanced trafficking signal and ER export signal …

    Promoter

    CaMKIIa

    Available since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pINDUCER12 (miR-RUL)

    Plasmid

    #46935

    Purpose

    Inducible lentiviral gene silencing vector. Insert PheSGly294, (XhoI to EcoRI;1.4kb),can be replaced w mir30 based hairpin of interest

    Depositor

    Thomas Westbrook

    Article

    Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

    Insert

    PheS Gly294
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLV-CMV-hXRCC1-mCherry-Hygro

    Plasmid

    #176532

    Purpose

    mCherry fused to the C-terminus of XRCC1 & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Wilk et al Sci Rep. 2020 Jan 20;10(1):651. doi: 10.

    Insert

    X-Ray Repair Cross Complementing 1 (XRCC1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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