We narrowed to 4,131 results for: p2a

1. pAAV-hSyn-hPOMC1-26-sbGLuc-P2A-dTomato

   Plasmid

   #176704

   Purpose

   neural activity dependent release of sbGLuc from presynaptic vesicles

   Depositor

   Ute Hochgeschwender

   Article

   Prakash et al Commun Biol. 2022 Jan 11;5(1):33. doi: 1

   Inserts

   Gaussia luciferase, slow burn
   dTomato
   

   Use

   AAV

   Tags

   P2A and hPOMC1-26

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDH-CB-iCre-P2A-tdTomato-T2A-Puro

   Plasmid

   #72255

   Purpose

   Express iCre, tdTomato and puromycin resistance gene (puromycin N-acetyl-transferase) under CB (chicken beta-actin + CMV enhancer) promoter

   Depositor

   Kazuhiro Oka

   Insert

   iCre-P2A-tdTomato-T2A-Puro
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CB and chicken beta-actin + CMV enhancer

   Available since

   March 1, 2017

   Availability

   Academic Institutions and Nonprofits only

3. pLV-UBC mTagBFP2-P2A-3xFLAG-hEEA1

   Plasmid

   #214995

   Purpose

   Express the human EndoTag (3xFLAG-EEA1) for EndoIPs. mTagBFP2 to sort for even expression levels.

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   EEA1 (EEA1 Human)
   

   Use

   Lentiviral

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. CROPseq-Guide-EFS-SpCas9-P2A-EGFP

   Plasmid

   #99248

   Purpose

   single-vector system with EGFP reporter for use in CROPseq knockout screens

   Depositor

   Alex Hewitt

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Nov. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-TIWB-myrmScarlet-I-P2A-post-eGRASP

   Plasmid

   #111584

   Purpose

   An AAV vector that expresses myristoylated mScarlet-I and post-eGRASP linked by self-cleaving P2A peptide under the tetO promoter.

   Depositor

   Bong-Kiun Kaang

   Article

   Choi et al Science. 2018 Apr 27;360(6387):430-435.

   Insert

   myrmScarlet-I-P2A-post-eGRASP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   tetO

   Available since

   June 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAF0692 Ef1a-Dn29_attD-Puro-p2a-mCherry

   Plasmid

   #193465

   Purpose

   Dn29 Donor with promoter for mcherry and puro expression

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   Dn29_attP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. PP_079_pAAV_CAG_LR-Voltron2-P2A-CheRiff-eGFP-ST (Kv2.1TS)

   Plasmid

   #230989

   Purpose

   Co-expressing membrane-localized Voltron2 and soma-localized CheRiff.

   Depositor

   Adam Cohen

   Article

   Wong-Campos et al bioRxiv [Preprint]. 2023 May 26:2023.05.

   Insert

   Membrane-localized Voltron2 and soma-localized CheRiff
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. PP_080_pAAV_CAG_DIO-LR-Voltron2-P2A-CheRiff-eGFP-ST (Kv2.1TS)

   Plasmid

   #230990

   Purpose

   Co-expressing membrane-localized Voltron2 and membrane-localized CheRiff.

   Depositor

   Adam Cohen

   Article

   Wong-Campos et al bioRxiv [Preprint]. 2023 May 26:2023.05.

   Insert

   Cre-dependent, membrane-localized Voltron2 and soma-localized CheRiff
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hSynapsin1-axon-jGCaMP8m-P2A-mRuby3

   Plasmid

   #172922

   Purpose

   For enriched expression of jGCaMP8m in axons with cytosolic expression of mRuby3

   Depositor

   GENIE Project

   Has Service

   AAV1 and AAV9

   Insert

   axon-jGCaMP8m-P2A-mRuby3
   

   Use

   AAV

   Tags

   GAP43 palmitoylation domain (N terminal on insert…

   Expression

   Mammalian

   Mutation

   Promoter

   hSynapsin1

   Available since

   Aug. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-FNLS-P2A-GFP-PGK-Puro

    Plasmid

    #110869

    Purpose

    Lentiviral vector for constitutive expression of FNLS-P2A-GFP in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE3RA-FNLS
    

    Use

    Lentiviral

    Tags

    3X FLAG

    Expression

    Mutation

    D10A and NLS sequence at the N-terminus

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. CMV:eGFP-p2A-HA-β2Ar-uTEV1Δ(220-242)

    Plasmid

    #135459

    Purpose

    Mammalian expression of SPARK protease component

    Depositor

    Alice Ting

    Article

    Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

    Insert

    eGFP-p2A-HA-βarrestin2-uTEV1Δ
    

    Use

    Lentiviral

    Tags

    eGFP, HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTR-346 pUC19-tNGFR-P2A-IL2RA N

    Plasmid

    #186065

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    IL2RA (IL2RA Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-T7-TadCBEd-SpCas9-P2A-EGFP (BKS327)

    Plasmid

    #223123

    Purpose

    pCMV and pT7 Human expression plasmid for TadCBEd-SpCas9 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadCBEd-SpCas9-2xUGI-P2A-EGFP
    

    Use

    CRISPR

    Tags

    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

    Expression

    Mammalian

    Mutation

    TadA-CDd mutations; nSpCas9=D10A

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-T7-TadCBEd-SpRY-P2A-EGFP (BKS428)

    Plasmid

    #223125

    Purpose

    pCMV and pT7 Human expression plasmid for TadCBEd-SpRY with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadCBEd-SpRY-2xUGI-P2A-EGFP
    

    Use

    CRISPR

    Tags

    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

    Expression

    Mammalian

    Mutation

    TadA-CDd mutations; nSpRY=D10A/A61R/L1111R/D1135L…

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-EF1a-mCherry-P2A-NLSFlpN-nMagHigh

    Plasmid

    #180585

    Purpose

    PA-Flp

    Depositor

    Won Do Heo

    Article

    Jung et al Nat Commun. 2019 Jan 18;10(1):314. doi:

    Insert

    Split FlpO
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV-hSyn-mCherry-P2A-Cre-WPRE

    Plasmid

    #107312

    Purpose

    Encoding hSyn-mCherry-Cre

    Depositor

    Hui Yang

    Article

    Zhou et al Nat Neurosci. 2018 Jan 15. pii: 10.1038/

    Has Service

    AAV1

    Insert

    hSyn-mCherry-P2A-Cre-WPRE
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV_hSyn-fDIO-somQuasAr6a-EGFP-P2A-sombC1C2TG

    Plasmid

    #183537

    Purpose

    Voltage indicator

    Depositor

    Karl Deisseroth

    Article

    Fan et al Cell. 2023 Feb 2;186(3):543-559.e19. doi

    Insert

    somQuasAr6a-EGFP-P2A-sombC1C2TG
    

    Use

    AAV

    Tags

    Expression

    Mutation

    None

    Promoter

    hSyn

    Available since

    Feb. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLCKO-CMV-Blasticidin-P2A-3xHA-TMEM106B_Human

    Plasmid

    #238130

    Purpose

    This plasmid encodes the full-length human TMEM106B protein fused with a 3xHA tag, along with an N-terminal blasticidin selection marker.

    Depositor

    Dirk Daelemans

    Article

    Baggen et al Cell. 2023 Aug 3;186(16):3427-3442.e22.

    Insert

    TMEM106B (TMEM106B Human)
    

    Use

    Lentiviral

    Tags

    Blasticidin-P2A-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEJS1084_pTetR-P2A-BFP/BspMI flexible sgRNA

    Plasmid

    #117687

    Purpose

    U6 driven Spy sgRNA cloning vector where guide sequences are inserted between BspMI sites. This plasmid works with Addgene #108570 for subnuclear proteomic profiling via C-BERST method.

    Depositor

    Erik Sontheimer

    Article

    Gao et al Methods Enzymol. 2019;616:365-383. doi:

    Inserts

    Kanamycin cassette
    TetR-P2A-BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and hPGK

    Available since

    Dec. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAV-hSyn1-GCaMP6f-P2A-nls-dTomato

    Plasmid

    #51085

    Purpose

    Neuronal expression of GCaMP6f Ca sensor and physically separate nuclear localized dTomato fluorophore

    Depositor

    Jonathan Ting

    Has Service

    AAV Retrograde and AAV1

    Insert

    GCaMP6f
    

    Use

    AAV

    Tags

    P2A-nls-dTomato

    Expression

    Mammalian

    Mutation

    Promoter

    human Synapsin1

    Available since

    Feb. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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