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We narrowed to 1,428 results for: transformation

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Showing: 1341 - 1360 of 1428 results

  1. pBrain-GFP-TACC3KDP(L566,567A)-shTACC3

    Plasmid
    #69117
    Purpose
    Dual-promoter plasmid to express knockdown-proof human TACC3 (L566A and L567A substitutions) tagged with EGFP along with shRNA to knock down endogenous TACC3 in mammalian cells.
    Depositor
    Stephen Royle
    Article
    Gutierrez-Caballero et al Biol Open. 2015 Jan 16;4(2):170-9. doi:
    Inserts
    Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
    shTACC3
    Use
    RNAi
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    LL (566-567aa) mutated to AA. Silent mutations i…
    Promoter
    CMV
    Available Since
    Feb. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    69117-plasmid-map-sequence-id-122092

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  2. pBrain-GFP-TACC3KDP(S558A)-shTACC3

    Plasmid
    #59357
    Purpose
    Expresses GFP-tagged human TACC3 (RNAi-resistant) with S558A substitution and knocks down endogenous TACC3 via shRNA.
    Depositor
    Stephen Royle
    Article
    Booth et al EMBO J. 2011 Mar 2;30(5):906-19. doi: 10
    Insert
    Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
    Use
    RNAi
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Silent mutations in amino acids 30-33 to confer s…
    Promoter
    CMV
    Available Since
    Dec. 5, 2014
    Availability
    Academic Institutions and Nonprofits only
    59357-plasmid-map-sequence-id-94887

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  8. pBrain-GFP-TACC3KDP∆678-681-shTACC3

    Plasmid
    #69115
    Purpose
    Dual-promoter plasmid to express knockdown-proof human TACC3 (amino acids 678-681 deleted) tagged with EGFP along with shRNA to knock down endogenous TACC3 in mammalian cells.
    Depositor
    Stephen Royle
    Article
    Gutierrez-Caballero et al Biol Open. 2015 Jan 16;4(2):170-9. doi:
    Inserts
    Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
    shTACC3
    Use
    RNAi
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Deletion of 678-681aa region. Silent mutations i…
    Promoter
    CMV
    Available Since
    Oct. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    69115-plasmid-map-sequence-id-122094

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  9. pUN1310 - pL2_pNOS-RLUC-tNOS_tOCS-FLUC-pDAT

    Plasmid
    #203902
    Purpose
    Reporter plasmid for the DAT promoter from C. roseus with firefly luciferase readout and Renilla luciferase normalization in a plant binary vector for Agrobacterium-mediated transformation.
    Depositor
    Carolyn Lee-Parsons
    Article
    Cole-Osborn et al Plant Mol Biol. 2025 Jun 5;115(3):72. do
    Insert
    pDAT-FLUC-tOCS; pNOS-RLUC-tNOS
    Use
    Luciferase
    Expression
    Plant
    Available Since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    203902-plasmid-map-sequence-id-404844

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  10. pUN1307 - pL2_pNOS-RLUC-tNOS_tOCS-FLUC-pT3R

    Plasmid
    #203899
    Purpose
    Reporter plasmid for the T3R promoter from C. roseus with firefly luciferase readout and Renilla luciferase normalization in a plant binary vector for Agrobacterium-mediated transformation.
    Depositor
    Carolyn Lee-Parsons
    Article
    Cole-Osborn et al Plant Mol Biol. 2025 Jun 5;115(3):72. do
    Insert
    pT3R-FLUC-tOCS; pNOS-RLUC-tNOS
    Use
    Luciferase
    Expression
    Plant
    Available Since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    203899-plasmid-map-sequence-id-404833

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  11. pUN1308 - pL2_pNOS-RLUC-tNOS_tOCS-FLUC-pNMT

    Plasmid
    #203900
    Purpose
    Reporter plasmid for the NMT promoter from C. roseus with firefly luciferase readout and Renilla luciferase normalization in a plant binary vector for Agrobacterium-mediated transformation.
    Depositor
    Carolyn Lee-Parsons
    Article
    Cole-Osborn et al Plant Mol Biol. 2025 Jun 5;115(3):72. do
    Insert
    pNMT-FLUC-tOCS; pNOS-RLUC-tNOS
    Use
    Luciferase
    Expression
    Plant
    Available Since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    203900-plasmid-map-sequence-id-404838

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  12. pSB284 - pL2_pNOS-RLUC-tNOS_tOCS-FLUC-pT16H2

    Plasmid
    #203896
    Purpose
    Reporter plasmid for the T16H2 promoter from C. roseus with firefly luciferase readout and Renilla luciferase normalization in a plant binary vector for Agrobacterium-mediated transformation.
    Depositor
    Carolyn Lee-Parsons
    Article
    Cole-Osborn et al Plant Mol Biol. 2025 Jun 5;115(3):72. do
    Insert
    pT16H2-FLUC-tOCS; pNOS-RLUC-tNOS
    Use
    Luciferase
    Expression
    Plant
    Available Since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    203896-plasmid-map-sequence-id-404824

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  13. pUN1305 - pL2_pNOS-RLUC-tNOS_tOCS-FLUC-p16OMT

    Plasmid
    #203897
    Purpose
    Reporter plasmid for the 16OMT promoter from C. roseus with firefly luciferase readout and Renilla luciferase normalization in a plant binary vector for Agrobacterium-mediated transformation.
    Depositor
    Carolyn Lee-Parsons
    Article
    Cole-Osborn et al Plant Mol Biol. 2025 Jun 5;115(3):72. do
    Insert
    p16OMT-FLUC-tOCS; pNOS-RLUC-tNOS
    Use
    Luciferase
    Expression
    Plant
    Available Since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    203897-plasmid-map-sequence-id-404828

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  14. pUN1306 - pL2_pNOS-RLUC-tNOS_tOCS-FLUC-pT3O

    Plasmid
    #203898
    Purpose
    Reporter plasmid for the T3O promoter from C. roseus with firefly luciferase readout and Renilla luciferase normalization in a plant binary vector for Agrobacterium-mediated transformation.
    Depositor
    Carolyn Lee-Parsons
    Article
    Cole-Osborn et al Plant Mol Biol. 2025 Jun 5;115(3):72. do
    Insert
    pT3O-FLUC-tOCS; pNOS-RLUC-tNOS
    Use
    Luciferase
    Expression
    Plant
    Available Since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    203898-plasmid-map-sequence-id-404831

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  15. pToxAmp12

    Plasmid
    #218286
    Purpose
    Multi-copy integration of heterologous genes (AeBlue and RelB) through co-transformation with ToxAmp (toxin-antitoxin-driven gene amplification) modules
    Depositor
    Claudia Vickers
    Article
    Evans et al bioRxiv 2024.01.30.578080
    Insert
    HO(-253, -1)-pCRT1>RelB>tPDC1-pTDH3>AeBlue>tsynth7-ARS712-HO(-731, -264)
    Expression
    Yeast
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218286-plasmid-map-sequence-id-433688

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  17. pJET1.2[eb-Tc’bhsp-EGFP-2A-Cre;3xP3-gTc’v-SV40]

    Plasmid
    #124068
    Purpose
    CRISPR/Cas mediated knock-in via non-homologous end-joining in Tribolium; bhsp drives EGFP expression; Dm-ebony for linearization; (Transformation plasmid; Black eye marker)
    Depositor
    Gregor Bucher
    Article
    He et al Elife. 2019 Oct 18;8. pii: 49065. doi: 1
    Insert
    eb-Tc’bhsp-EGFP-2A-Cre; 3xP3-gTc’v-SV40
    Use
    CRISPR and Cre/Lox
    Expression
    Insect
    Promoter
    bhsp68
    Available Since
    July 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    124068-plasmid-map-sequence-id-240659

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Showing: 1341 - 1360 of 1428 results