We narrowed to 24,310 results for: Fus
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Plasmid#111194Purposefluorescent fusion proteinDepositorInsertIKB-alpha
TagsEYFPExpressionMammalianAvailable SinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNSSCPG
Plasmid#183187PurposePACE-evolved split N (core) terminal of T7 RNA polymerase-SpmXΔC and SpmXΔC-PACE-evolved split C (sigma) terminal of T7 RNA polymerase both expressed under Tac promoter; sfGFP-LAA expressed under T7 pDepositorInsertssuperfolder GFP
SpmX450 and T7 181+ fusion protein
T7 RNAP 1-179 variant and SpmX450 fusion protein
mRFP1 and popZ fusion protein
ExpressionBacterialAvailable SinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR P4-P1R-mKate2
Plasmid#48344PurposeContributes the coding sequence for the mKate2 fluorescent protein as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.DepositorInsertmKate2
UseGateway entry vectorMutationIt contains a Kozak sequence but no stop codon.PromoternoneAvailable SinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
Cx43-sfGFP
Plasmid#70053PurposeExpresses rat Cx43 (Gja1 CDS) tagged with non-monomerized sfGFP on the C-terminus. CMV promoter.DepositorInsertCx43 (Gja1 Rat)
Tagsnon-monomerized superfolder GFP (V207)Mutationfused in frame to non-monomerized superfolder GFPPromoterCMVAvailable SinceOct. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBT3019
Plasmid#122541PurposeExpression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a peroxisome target peptide (SKL) under the intestinal specific promoter nhx-2DepositorInsertPnhx-2_CemOrange2::SKL
TagsSKLExpressionBacterialPromoterPnhx-2Available SinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
hRad51(A190L-A192L)–Cas9(D10A)
Plasmid#125562PurposeExpresses the Rad51(A190L-A192L)–Cas9(D10A) fusion construct in mammalian cells under CMV promoterDepositorAvailable SinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
LLP250 pEF1a-TALE-SunTag
Plasmid#100939PurposeEmpty TALE vector fused to SunTagx10 driven by EF1a promoter, with 3xHA tag, no PURO geneDepositorInsertTALE insertion site, SunTag array (10xGCN4)
UseTALENTags3xHAExpressionMammalianAvailable SinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCSD_SpCas9MT3-NLS-3xHA-NLS-SaCas9WT-2xNLS
Plasmid#107322PurposeExpresses SpCas9 (R1335K) fused to SaCas9 in mammalian cellsDepositorInsertSpCas9-SaCas9 fusion
UseCRISPRTagsNLSExpressionMammalianMutationSpCas9 (R1335K) is fused to SaCas9PromoterCMV IE93Available SinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCSD_2XNLS-SpCas9MT3-NLS-3xHA-2XNLS-NmCas9WT-2XNLS
Plasmid#107325PurposeExpresses SpCas9 (R1335K) fused to NmCas9 in mammalian cellsDepositorInsertSpCas9-NmCas9 fusion
UseCRISPRTagsNLSExpressionMammalianMutationSpCas9 (R1335K) is fused to NmCas9PromoterCMV IE94Available SinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCSD_2XNLS-SpCas9MT2-NLS-3xHA-2XNLS-NmCas9WT-2XNLS
Plasmid#107326PurposeExpresses SpCas9 (R1333S) fused to NmCas9 in mammalian cellsDepositorInsertSpCas9-NmCas9 fusion
UseCRISPRTagsNLSExpressionMammalianMutationSpCas9 (R1333S) is fused to NmCas9PromoterCMV IE93Available SinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only