We narrowed to 7,189 results for: cas9 plasmid
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Plasmid#109391PurposeThis construct encodes for two units. The first contains a constitutively expressed dCas9 protein. The second contains an sgRNA cassette under the control of the burden-responsive htpG1 promoter.DepositorInsertpJ23100mut-dCas9-T- phtpG1-sgRNA
ExpressionBacterialAvailable SinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
LLP778_Traptavidin-p65HSF1-CO
Plasmid#211771PurposeAviTag counterpart binding domain, Traptavidin, fused to transcriptional activator p65HSF1, with sfGFP selectionDepositorInsertTraptavidin-p65HSF1
Tags3xV5ExpressionMammalianMutationLast 300 bp codon-optimised (CO) to detect p65HSF…PromoterpEF1a and pSV40Available SinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
LLP776_SpyCatcher-p65HSF1-CO
Plasmid#211769PurposeSpyTag counterpart binding domain, SpyCatcher, fused to transcriptional activator p65HSF1, with GFP selectionDepositorInsertSpyCatcher-p65HSF1
Tags3xFLAGExpressionMammalianMutationLast 300 bp codon-optimised (CO) to detect p65HSF…PromoterpEF1a and pSV40Available SinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
LLP783_SpyCatcher-DNMT3A-CO
Plasmid#211776PurposeSpyTag counterpart binding domain, SpyCatcher, fused to DNA methyltransferase DNMT3A, with GFP selectionDepositorInsertTraptavudin-DNMT3A
Tags3xV5ExpressionMammalianMutationLast 300 bp codon-optimised (CO) to detect DNMT3A…PromoterpEF1a and pSV40Available SinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
JI401: Ad5 E1A/Hexon qRT-PCR standard
Plasmid#131753PurposePlasmid standard containing regions of the Ad5 E1A and Ad5 Hexon genes to create a qRT-PCR standard curve for detection of replication competent adenovirus (RCA).DepositorInsertsAd5 E1A gene fragment
Ad5 Hexon gene fragment
UseStandard for qrt-pcrPromoterN/AAvailable SinceOct. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
LLP784_SnoopCatcher-DNMT3A-CO
Plasmid#211777PurposeSnoopTag counterpart binding domain, SnoopCatcher, fused to DNA methyltransferase DNMT3A, with GFP selectionDepositorInsertSpy-EZH2
Tags3xFLAGExpressionMammalianMutationLast 300 bp codon-optimised (CO) to detect EZH2 e…PromoterpEF1a and pSV40Available SinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
LLP780_SpyCatcher-KRAB-CO
Plasmid#211773PurposeSpyTag counterpart binding domain, SpyCatcher, fused to transcriptional repressor KRAB, with GFP selectionDepositorInsertSpyCatcher-KRAB
Tags3xFLAGExpressionMammalianMutationLast 300 bp codon-optimised (CO) to detect KRAB e…PromoterpEF1a and pSV40Available SinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJEP301-pAAV-CMV-MCS3-pA
Plasmid#113676PurposeCMV driven Multi Cloning Site-3DepositorInsertN/A
UseAAVPromoterCytomegalo Virus(CMV)Available SinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
LLP789_αGCN4-DNMT3A-CO
Plasmid#211783PurposeSunTag counterpart binding domain, aGCN4, fused to DNA methyltransferase DNMT3A, with GFP selectionDepositorInsertaGCN4-DNMT3A
Tags3xTy1ExpressionMammalianMutationLast 300 bp codon optimised (CO) to detect DNMT3A…PromoterpEF1a and pSV40Available SinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJEP306-pAAV-EFS-MCS3-pA
Plasmid#113682PurposeEFS driven Multi Cloning Site-3.DepositorInsertN/A
UseAAVPromoterCytomegalo Virus(CMV)Available SinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only