We narrowed to 43,339 results for: Sele
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Plasmid#119710PurposeTU of the HSVtk negative marker for F2dU domesticated into pUPD2 with GGAG/TACT barcodes (reverse orientation). Used for gene KO with dual selec. According to FungalBraid modular DNA assembly for ATMTDepositorInsertNegative selection marker HSVtk (Pgpda:HSVtk:Ttub)
UseSynthetic Biology; Domestication of dna parts for…Available SinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28A-p53 (1-73) (P12A P13A)
Plasmid#62066PurposeExpression of human p53 (residues 1-73) (P12A, P13A) in e. coliDepositorInsertp53 (TP53 Human)
TagsHisExpressionBacterialMutationContains TAD residues 1-73; P12A, P13APromoterT7Available SinceFeb. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTT5-hSDC1-CS2
Plasmid#52367Purposeexpresses human Syndecan-1 point mutant at S194A in HEK293-EBNA1 (293E) suspension culture.DepositorInsertSDC1 (SDC1 Human)
ExpressionMammalianMutationR95Q and S194A (according to numbering in Fig. 2A…Available SinceDec. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEM-NLS-BirA-2A-mCherry-SV40pA-FKF
Plasmid#79889PurposeDonor cassette containing HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection geneDepositorInsertHA-tagged NLS-BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
ExpressionBacterialAvailable SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYPQ284-v1
Plasmid#138118PurposeGateway entry plasmid (attL1 & attR5) expressing rice codon optimized Mb2Cas12a with D172R mutation, without promoterDepositorInsertMb2Cas12a v1
UseCRISPR; Gateway compatible cas12a entry cloneTagsNLSExpressionPlantMutationD172RAvailable SinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
attB-mCh-Dnmt3b1-Poly(A)-NeoR
Plasmid#65553PurposePlasmid for Bxb1-mediated recombination of the mCh-Dnmt3b1 cDNA into a MIN-tagged locus using Neomycin selectionDepositorInsertDnmt3b (Dnmt3b Mouse)
UseMouse Targeting; Bxb1TagsmCherryExpressionMammalianMutationisoform 1Available SinceAug. 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYPQ223-STU
Plasmid#138109PurposeGateway entry plasmid (attL1 & attR5) expressing rice codon optimized, catalytically deactivated AsCas12a-SRDX repressor without promoter and terminatorDepositorInsertdAsCas12a-SRDX
UseCRISPR; Gateway compatible cas12a entry cloneTagsNLSExpressionPlantAvailable SinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
attB-GFP-Dnmt3b1-Poly(A)-NeoR
Plasmid#65551PurposePlasmid for Bxb1-mediated recombination of the GFP-Dnmt3b1 cDNA into a MIN-tagged locus using Neomycin selectionDepositorInsertDnmt3b (Dnmt3b Mouse)
UseMouse Targeting; Bxb1TagsGFPExpressionMammalianMutationisoform 1Available SinceAug. 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET21-Drp1(deltaIB)
Plasmid#73041PurposeBacterial expression of mouse Drp1 isoform b (minus residues 514-602) with C-terminal His-tag and PreScission protease cleavage siteDepositorInsertDrp1(deltaIB) (Dnm1l Mouse)
Tags6xHis-tag and PreScission Protease siteExpressionBacterialMutationDeleted amino acids 514-602PromoterT7Available SinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(hEGFR_2b)-PGKpuro2ABFP-W
Plasmid#208432PurposeLentiviral gRNA plasmid targeting human EGFR gene, co-expression of BFP tagDepositorInsertEGFR (EGFR Human)
UseCRISPR and LentiviralExpressionMammalianMutationN/APromoterhuman U6Available SinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only