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We narrowed to 14,129 results for: Ott;

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pEAHISMRSA1376

Plasmid

#97002

Purpose

Expressing MRSA sar1376 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

Putative 4-oxalocrotonate tautomerase

Use

Tags

N-ter TEV protease cleavable 6His

Expression

Mutation

none

Promoter

T7

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISPSVORF131

Plasmid

#97022

Purpose

Expressing Pyrobaculum spherical virus orf131 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

PSV orf 131

Use

Tags

N-ter TEV protease cleavable 6HIs

Expression

Mutation

none

Promoter

T7

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISPA4511

Plasmid

#97015

Purpose

Expressing Pseudomonas aeruginosa orf PA4511 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

P. aeruginosa orf PA4511

Use

Tags

N-ter TEV protease cleavable 6HIs

Expression

Mutation

none

Promoter

T7

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISMRSATAG

Plasmid

#97004

Purpose

Expressing MRSA TAG protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

DNA-3-methyladenine glycosylase I

Use

Tags

N-ter TEV protease cleavable 6His

Expression

Mutation

Promoter

T7

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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p2706-pHAGE-EF1aL-YAP5SA-UBC-GFP

Plasmid

#216475

Purpose

lentiviral dual expression of YAP5SA and eGFP

Depositor

Darrell Kotton

Article

Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

Inserts

YAP5SA
eGFP

Use

Lentiviral

Tags

Expression

Mutation

S61A, S109A, S127A, S164A, S381A

Promoter

EF1aL and UBC

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pSCGB3A2_HL-P2A-TagBFP-PGK-NeoR-SCGB3A2_HR

Plasmid

#126697

Purpose

donor vector for targeting a 2A peptide followed by blue fluorescent reporter to the human SCGB3A2 locus at the end of the endogenous coding sequence

Depositor

Darrell Kotton

Article

McCauley et al Stem Cell Reports. 2018 May 8;10(5):1579

Insert

TagBFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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EGFP-HsBIN1_tv8

Plasmid

#176012

Purpose

Mammalian expression of Human EGFP-Bin1 (transcriptional variant 8, muscle specific). Parton lab clone HBH

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

Bin1

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pME-GcAMP5

Plasmid

#126569

Purpose

Multisite gateway middle entry clone containing zebrafish calcium reporter GcAMP5. Parton lab clone ANR.

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

GcAMP5

Use

Gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-Bin1b

Plasmid

#176014

Purpose

Mammalian expression of zebrafish EGFP-Bin1b. Parton lab clone HYG

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

Bin1b (bin1b Zebrafish)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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p2705-AGER-eGFP

Plasmid

#216474

Purpose

donor vector for targeting an eGFP reporter to the human AGER locus at the endogenous ATG start site

Depositor

Darrell Kotton

Article

Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

Insert

eGFP

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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SITS-EGFP-Bin1a

Plasmid

#176020

Purpose

Leishmania cell free expression of zebrafish EGFP-Bin1a. Parton lab clone GRQ

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

Bin1a

Use

Leishmania cell free expression

Tags

EGFP

Expression

Mutation

Promoter

Available since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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SITS-mCherry-Bin1a

Plasmid

#176024

Purpose

Leishmania cell free expression of zebrafish mCherry-Bin1a. Parton lab clone GSS

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

Bin1a

Use

Leishmania cell free expression

Tags

mCherry

Expression

Mutation

Promoter

Available since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pET30(a)-AtNUDX23

Plasmid

#175021

Purpose

Purification of AtNUDX23 enzyme

Depositor

Jeppe Vinther

Article

Sherwood et al Nature. 2023 Jul;619(7971):811-818. doi:

Insert

AtNUDX23 (NUDX23 Mustard Weed)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE2-EF1aL-Cre-IRES-NeoR-W

Plasmid

#126700

Purpose

lentiviral or plasmid based co-expression of Cre recombinase and a neomycin resistance cassette

Depositor

Darrell Kotton

Article

Jacob et al Cell Stem Cell. 2017 Oct 5;21(4):472-488

Insert

Cre-IRES

Use

Cre/Lox and Lentiviral

Tags

SV40 NLS

Expression

Mutation

Promoter

Ef1a

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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Cavin4-mCherry

Plasmid

#176007

Purpose

Mammalian expression of Mouse Cavin4-mCherry. Parton lab clone JSG

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

Cavin4 (Cavin4 Mouse)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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EF1a-TALEN_HD (SPC right)

Plasmid

#126695

Purpose

right TALENS for introducing a double strand break into the genomic human SFTPC locus at the ATG start site

Depositor

Darrell Kotton

Article

Jacob et al Cell Stem Cell. 2017 Oct 5;21(4):472-488

Insert

human SPC right

Use

TALEN

Tags

NLS, truncated N term and TAL, FokI

Expression

Mammalian

Mutation

R451P in SPC

Promoter

EF1

Available since

April 11, 2022

Availability

Academic Institutions and Nonprofits only

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EF1a-TALEN_NN (SPC left)

Plasmid

#126694

Purpose

left TALENS for introducing a double strand break into the genomic human SFTPC locus at the ATG start site

Depositor

Darrell Kotton

Article

Jacob et al Cell Stem Cell. 2017 Oct 5;21(4):472-488

Insert

human SPC left

Use

TALEN

Tags

0.5 repeat NN, TAL, FokI and NLS, truncated N ter…

Expression

Mammalian

Mutation

Promoter

EF1

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pHL-EF1a-SphcCas9(D10A)-iP-A

Plasmid

#60600

Purpose

Expresses D10A mutant (nickase) of human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.

Depositor

Akitsu Hotta

Article

Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

Inserts

CRISPR Cas9 D10A
puromycin resistance gene

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Changed Asp 10 to Ala, Codon usage optimized for …

Promoter

Available since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pEAHISMRSA0482

Plasmid

#96968

Purpose

expression of methicillin-resistant Staphylococcus aureus orf 0482

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

MRSA ORF0482

Use

Tags

N-ter TEV protease cleavable 6His

Expression

Bacterial

Mutation

none

Promoter

T7

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISMRSA2028

Plasmid

#97001

Purpose

Expressing MRSA Asp/Tyr/Phe pyridoxal-50- phosphate-dependent aminotransferase

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

Asp/Tyr/Phe pyridoxal-50- phosphate-dependent aminotransferase

Use

Tags

N-ter TEV protease cleavable 6His

Expression

Mutation

none

Promoter

T7

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge