We narrowed to 3,478 results for: Ubc

1. pDY156: SUZ12 px330 sgRNA Cas9 plasmid

   Plasmid

   #170792

   Purpose

   This plasmid encodes Cas9 and a sgRNA that targets the SUZ12 locus; to be used with pDY153

   Depositor

   Thomas Cech

   Article

   Youmans et al Mol Cell. 2021 Feb 4;81(3):488-501.e9. d

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX6p1 AO7R

   Plasmid

   #137842

   Purpose

   GST tagged AO7 encoding aa 126-216 for expression in bacteria

   Depositor

   Allan Weissman

   Article

   Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

   Insert

   AO7 (RNF25 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   March 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. MAC_C_MRM1

   Plasmid

   #111671

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

   Insert

   MRM1 (MRM1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pQE30-roGFP1-iR

   Plasmid

   #83314

   Purpose

   Inducible expression of redox sensitive GFP in bacteria

   Depositor

   S. James Remington

   Article

   Lohman et al Biochemistry. 2008 Aug 19;47(33):8678-88

   Insert

   roGFP1-iR
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   ro1(C48S/S147C/Q204C) plus X insertion, iR betwe…

   Promoter

   T5

   Available since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pCR8-DJ1-E64D

   Plasmid

   #29356

   Depositor

   Mark Cookson

   Article

   Blackinton et al Brain Res Mol Brain Res. 2005 Mar 24. 13

   Insert

   DJ1 (PARK7 Human)
   

   Use

   Entry vector

   Tags

   Expression

   Mutation

   Glu64Asp

   Promoter

   Available since

   March 31, 2011

   Availability

   Academic Institutions and Nonprofits only

6. pCAGGS_cAMPFIRE-M

   Plasmid

   #182284

   Purpose

   Mammalian expression of the cAMP sensor cAMPFIRE-M under a CAG promotor w/ WPRE translation enhancer.

   Depositor

   Haining Zhong

   Article

   Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

   Insert

   cAMPFIRE-M (cAMP sensor)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Cyto-roGFP

   Plasmid

   #49435

   Purpose

   Expresses thiol redox-sensitive ratiometric sensor roGFP in cytosol of mammalian cells

   Depositor

   Paul Schumacker

   Article

   Waypa et al Circ Res. 2010 Feb 19;106(3):526-35. doi

   Insert

   roGFP2
   

   Use

   Adenoviral

   Tags

   Expression

   Mammalian

   Mutation

   C48S, Q80R, S147C, and Q204C in Clontech's G…

   Promoter

   CMV

   Available since

   Dec. 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV R-CEPIA1er

   Plasmid

   #58216

   Purpose

   Red fluorescent indicator for calcium signaling in the endoplasmic reticulum

   Depositor

   Masamitsu Iino

   Article

   Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

   Insert

   R-CEPIA1er
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   R-GECO1 E300D M305L D329N F332I E336D N346K F361W…

   Promoter

   CMV

   Available since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. Golgi-TurboID

   Plasmid

   #200959

   Purpose

   Transiently expresses golgi localized TurboID with HA tags

   Depositor

   Keriann Backus

   Article

   Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

   Insert

   Golgi-TurboID
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1_GEM2-Halo-FRB

    Plasmid

    #197056

    Purpose

    Expression of Synechococcus elongatus encapsulin Halo-FRB-fusion in mammalian cells

    Depositor

    Julia Mahamid

    Article

    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

    Insert

    Encapsulin
    

    Use

    Tags

    FRB and HaloTag

    Expression

    Mammalian

    Mutation

    Codon-optimised for human cell expression

    Promoter

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV G-CEPIA1er

    Plasmid

    #58215

    Purpose

    Green fluorescent indicator for calcium imaging in the endoplasmic reticulum

    Depositor

    Masamitsu Iino

    Article

    Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

    Insert

    G-CEPIA1er
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    G-GECO1.1 E299D M304L D328N N345K F360W G369S E37…

    Promoter

    CMV

    Available since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAGGS_cAMPFIRE-L

    Plasmid

    #182283

    Purpose

    Mammalian expression of the cAMP sensor cAMPFIRE-L under a CAG promotor w/ WPRE translation enhancer.

    Depositor

    Haining Zhong

    Article

    Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

    Insert

    cAMPFIRE-L (cAMP sensor)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-PM-ExRai-CKAR2

    Plasmid

    #236098

    Purpose

    Plasma membrane-targeted enhanced excitation-ratiometric biosensor for monitoring Protein Kinase C activity in living cells.

    Depositor

    Jin Zhang

    Article

    Su et al Nat Chem Biol. 2024 Oct 11. doi: 10.1038

    Insert

    Lyn-ExRai-CKAR2
    

    Use

    Tags

    N-terminal targeting sequence from Lyn kinase

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-Cyto-ExRai-CKAR2

    Plasmid

    #236097

    Purpose

    Cytosol-targeted enhanced excitation-ratiometric biosensor for monitoring Protein Kinase C activity in living cells.

    Depositor

    Jin Zhang

    Article

    Su et al Nat Chem Biol. 2024 Oct 11. doi: 10.1038

    Insert

    ExRai-CKAR2-NES
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. lenti-mito-mTagBFP2

    Plasmid

    #216130

    Purpose

    Lentiviral vector for expressing mTagBFP2 localizing to mitochondria.

    Depositor

    Stefan Kubicek

    Article

    Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

    Insert

    EF1a-COX8AmitochondrialLocalizationSignal-mTagBFP2-WPRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. tdTomato-anti-AlfaTag nanobody

    Plasmid

    #171819

    Purpose

    Bacterial expression vector for production of fluorescent anti-AlfaTag nanobody

    Depositor

    Ikuo Wada

    Article

    Matsumoto et al J Biol Chem. 2021 Jul;297(1):100851. doi

    Insert

    tdTomato-anti-AlfaTag nanobody
    

    Use

    Tags

    6xHis and SEP tag (6xD)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. cfSGFP2-anti-AlfaTag nanobody

    Plasmid

    #171818

    Purpose

    Bacterial expression vector for production of fluorescent anti-AlfaTag nanobody

    Depositor

    Ikuo Wada

    Article

    Matsumoto et al J Biol Chem. 2021 Jul;297(1):100851. doi

    Insert

    cfSGFP2-anti-AlfaTag nanobody
    

    Use

    Tags

    6xHis and SEP tag (6xD)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLVX-EF1a-EGFP-RAB11B-IRES-Puromycin

    Plasmid

    #134860

    Purpose

    Expresses EGFP-tagged recycling endosome marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    RAB11B (RAB11B Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV CEPIA3mt

    Plasmid

    #58219

    Purpose

    Green fluorescent indicator for calcium imaging in the mitochondria (moderately low affinity variant)

    Depositor

    Masamitsu Iino

    Article

    Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

    Insert

    CEPIA3mt
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    GCaMP2 N263Y E282V E334D M339L

    Promoter

    CMV

    Available since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. FUGW-Cytosol-TurboID

    Plasmid

    #200961

    Purpose

    3rd gen lentiviral plasmid for expressing cytosol localized TurboID with EGFP

    Depositor

    Keriann Backus

    Article

    Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

    Insert

    Cytosol-TurboID
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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