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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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Showing: 1361 - 1380 of 15937 results

  1. pGEX-6P-1-WT-short-UBASH3A

    Plasmid
    #192104
    Purpose
    Bacterial expression of human wild-type short-form UBASH3A with GST tag
    Depositor
    Patrick Concannon
    Insert
    UBASH3A (UBASH3A Human)
    Use
    Tags
    GST
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Dec. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    192104-plasmid-map-sequence-id-379911

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  2. pLV-SFFV-mCherry-WPRE-UbC-Emerald

    Plasmid
    #211797
    Purpose
    Lentiviral vector plasmid expressing mCherry under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Richard Eva, Bart Nieuwenhuis
    Inserts
    mCherry
    Emerald
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV and UbC
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    211797-plasmid-map-sequence-id-418065

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  3. pDONR221-deltaUBA-short-UBASH3A-Ctag

    Plasmid
    #195397
    Purpose
    Human short-form UBASH3A lacking the UBA domain in Gateway pDONR221 (for C-terminal tag)
    Depositor
    Patrick Concannon
    Insert
    UBASH3A (UBASH3A Human)
    Use
    Gateway donor vector
    Tags
    Expression
    Mutation
    UBASH3A mutant lacking the UBA domain
    Promoter
    Available since
    March 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    195397-plasmid-map-sequence-id-388659

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  4. pDONR221-deltaUBA-short-UBASH3A-Ntag

    Plasmid
    #195398
    Purpose
    Human short-form UBASH3A lacking the UBA domain in Gateway pDONR221 (for N-terminal tag)
    Depositor
    Patrick Concannon
    Insert
    UBASH3A (UBASH3A Human)
    Use
    Gateway donor vector
    Tags
    Expression
    Mutation
    UBASH3A mutant lacking the UBA domain
    Promoter
    Available since
    March 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    195398-plasmid-map-sequence-id-386315

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  5. pGEX-6P-1-PDE-short-UBASH3A

    Plasmid
    #195403
    Purpose
    Bacterial expression of a putative PDE domain of human short-form UBASH3A with GST tag
    Depositor
    Patrick Concannon
    Insert
    UBASH3A (UBASH3A Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    PDE domain of UBASH3A
    Promoter
    Available since
    March 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    195403-plasmid-map-sequence-id-386313

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  6. deltaUBA-short-UBASH3A-V5-6xHis

    Plasmid
    #195404
    Purpose
    Mammalian expression of human short-form UBASH3A lacking the UBA domain with V5-6xHis tag
    Depositor
    Patrick Concannon
    Insert
    UBASH3A (UBASH3A Human)
    Use
    Tags
    V5-6xHis
    Expression
    Mammalian
    Mutation
    UBASH3A mutant lacking the UBA domain
    Promoter
    Available since
    March 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    195404-plasmid-map-sequence-id-386311

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  8. pT3-EF1aH-PIK3CA H1047R

    Plasmid
    #180031
    Purpose
    This plasmid is in the pT3-EF1a vector without loxP sites flanking the inverted repeats of SB (sleeping beauty) sequence. Therefore this plasmid can be used in LoxP mice.
    Depositor
    Xin Chen
    Article
    Wang et al Liver Int. 2015 Dec 30. doi: 10.1111/liv
    Insert
    PIK3CA H1047R (PIK3CA Human)
    Use
    Tags
    HA tag
    Expression
    Mammalian
    Mutation
    H1047R
    Promoter
    EF1a
    Available since
    March 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    180031-plasmid-map-sequence-id-357864

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  10. BPV00500 pET21GG2 His(6)-TF-BECN(1-450)

    Plasmid
    #136684
    Purpose
    Expresses the full-length Beclin-1 protein (a.a. 1-450) with a N-terminal hexa-Histidine and solubility tag (i.e., Trigger Factor)
    Depositor
    Colin Garvie
    Article
    Ranaghan et al Biochemistry. 2017 Dec 26;56(51):6639-66
    Insert
    Beclin-1 (BECN1 Human)
    Use
    Tags
    Trigger Factor
    Expression
    Bacterial
    Mutation
    DNA optimized for E. coli expression
    Promoter
    T7
    Available since
    Feb. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    136684-plasmid-map-sequence-id-272124

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Showing: 1361 - 1380 of 15937 results