We narrowed to 15,801 results for: nans

1. pET-28b-RfxCas13d-His

   Plasmid

   #141322

   Purpose

   Plasmid for bacterial expression and purification of RfxCas13d protein

   Depositor

   Miguel Angel Moreno-Mateos, Ariel Bazzini

   Article

   Kushawah et al Dev Cell. 2020 Sep 28;54(6):805-817.e7.

   Insert

   Rfx-Cas13d
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. sgAPC(A)

   Plasmid

   #85557

   Purpose

   Expresses sgRNA targeting human APC around codon I1417

   Depositor

   Luca Grumolato

   Article

   Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

   Insert

   APC, WNT signaling pathway regulator (APC Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

3. sgAPC(B)

   Plasmid

   #85558

   Purpose

   Expresses sgRNA targeting human APC around codon I1417

   Depositor

   Luca Grumolato

   Article

   Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

   Insert

   APC, WNT signaling pathway regulator (APC Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

4. pT3TS-RfxCas13d-NLS-HA

   Plasmid

   #141321

   Purpose

   Plasmid to carry out IVT of RfxCas13d-NLS (human codon-optimized)

   Depositor

   Miguel Angel Moreno-Mateos, Ariel Bazzini

   Article

   Kushawah et al Dev Cell. 2020 Sep 28;54(6):805-817.e7.

   Insert

   Rfx-Cas13d (NLS-RfxCas13d-NLS)
   

   Use

   CRISPR

   Tags

   HA and NLS

   Expression

   Mutation

   Promoter

   Available since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. sgTP53(R273)

   Plasmid

   #85561

   Purpose

   Expresses sgRNA targeting human TP53 around codon R273

   Depositor

   Luca Grumolato

   Article

   Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

   Insert

   tumor protein p53 (TP53 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

6. pmEos2-N1 PH-PLCd (wild-type) (PH-PLCd-mEos2)

   Plasmid

   #162877

   Purpose

   Expression in mammalian cells of Plekstrin-homology domain of Phospholipase C delta tagged with mEos2 to perform sptPALM

   Depositor

   Frederic Meunier

   Article

   Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

   Insert

   pleckstrin homology domain of PLCδ1 (phospholipase C-δ1) (PLCD1 Human)
   

   Use

   Tags

   mEos2

   Expression

   Mammalian

   Mutation

   Insert consists of AA1-170

   Promoter

   Available since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP-C1 PA-PLA1 (EGFP-PA-PLA1)

   Plasmid

   #162880

   Purpose

   Expression in mammalian cells of Phosphatidic Acid preferring Phospholipase A1 tagged with EGFP

   Depositor

   Frederic Meunier

   Article

   Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

   Insert

   Phosphatidic acid-preferring phospholipase A1 (PA-PLA1) (DDHD1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. sgTP53(A)

   Plasmid

   #85559

   Purpose

   Expresses sgRNA targeting human TP53 around codon F109

   Depositor

   Luca Grumolato

   Article

   Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

   Insert

   tumor protein p53 (TP53 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

9. pCMV-T7-NLS(SV40)-(Spyo_Cas9)-6xHis-NLS(SV40)

   Plasmid

   #185706

   Purpose

   CMV and T7 promoter expression plasmid for human codon usage anCas(Spyo_Cas9) with an N-terminal NLS and C-terminal NLS

   Depositor

   Raul Perez-Jimenez

   Article

   Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

   Insert

   Cas9
   

   Use

   Tags

   6xHis-NLS and NLS

   Expression

   Mammalian

   Mutation

   optimized for human codon usage

   Promoter

   CMV and T7

   Available since

   June 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pPICZc-HsACTB*-thymosinB-8His

    Plasmid

    #111146

    Purpose

    Expresses human beta-actin fused with thymosin beta and a His tag.

    Depositor

    Mohan Balasubramanian

    Article

    Hatano et al J Cell Sci. 2018 Mar 13. pii: jcs.213827

    Insert

    ACTB (ACTB Human)
    

    Use

    Pichia pastoris integration

    Tags

    Thymosin beta and a His-tag

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPICZc-HsACTG1*-thymosinB-8His

    Plasmid

    #111147

    Purpose

    Expresses human gamma-actin (codon is optimised for expression in Pichia pastoris) fused with thymosin beta and a His tag.

    Depositor

    Mohan Balasubramanian

    Article

    Hatano et al J Cell Sci. 2018 Mar 13. pii: jcs.213827

    Insert

    actin gamma 1 (ACTG1 Human)
    

    Use

    Pichia pastoris integration vector

    Tags

    Thymosin beta and a His-tag

    Expression

    Yeast

    Mutation

    codon-optimised for expression in Pichia pastoris

    Promoter

    AOX1

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-CMV-Blast-DNFGFR1-HA

    Plasmid

    #130887

    Purpose

    Expresses dominant negative mutant mouse FGFR1

    Depositor

    Gian-Paolo Dotto

    Article

    Bordignon et al Cell Rep. 2019 Aug 27;28(9):2358-2372.e6

    Insert

    Fgfr1 (Fgfr1 Mouse)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    Dominant Negative receptor lacking cytosolic cata…

    Promoter

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. sgTP53(B)

    Plasmid

    #85560

    Purpose

    Expresses sgRNA targeting human TP53 around codon F109

    Depositor

    Luca Grumolato

    Article

    Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

    Insert

    tumor protein p53 (TP53 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

14. sgEGFR(A)

    Plasmid

    #85564

    Purpose

    Expresses sgRNA targeting human EGFR around codon T790

    Depositor

    Luca Grumolato

    Article

    Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

    Insert

    epidermal growth factor receptor (EGFR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

15. pPICZc-Ub-R-HsACTB*-thymosinB-8His

    Plasmid

    #111149

    Purpose

    Expresses arginylated human beta-actin fused with thymosin beta and a His tag.

    Depositor

    Mohan Balasubramanian

    Article

    Hatano et al J Cell Sci. 2018 Mar 13. pii: jcs.213827

    Insert

    ACTB (ACTB Human)
    

    Use

    Pichia pastoris integration plasmid

    Tags

    Thymosin beta and a His-tag

    Expression

    Yeast

    Mutation

    N-terminal arginylation

    Promoter

    AOX1

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMT-HGP-v3-Nb127D01-hIgG

    Plasmid

    #171564

    Purpose

    Expresses hIgG-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-human IgG-TEV-Avi-His) in fly cells

    Depositor

    Hidde Ploegh, Norbert Perrimon

    Article

    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

    Insert

    Nb127D01-human IgG-TEV-Avi-His (CXCR2 Human)
    

    Use

    Tags

    BiP signal peptide and hIgG1-hinge-N297A-TEV site…

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. sgALK(Chr. Inv.)

    Plasmid

    #85569

    Purpose

    Expresses sgRNA targeting human ALK between exon 20 and 21

    Depositor

    Luca Grumolato

    Article

    Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

    Insert

    anaplastic lymphoma receptor tyrosine kinase (ALK Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

18. sgEGFR(B)

    Plasmid

    #85565

    Purpose

    Expresses sgRNA targeting human EGFR around codon T790

    Depositor

    Luca Grumolato

    Article

    Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

    Insert

    epidermal growth factor receptor (EGFR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

19. pCMV-T7-NLS(SV40)-anCas(FCA)-6xHis-NLS(SV40)

    Plasmid

    #185701

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage anCas(FCA) with an N-terminal NLS and C-terminal NLS

    Depositor

    Raul Perez-Jimenez

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    anCas(FCA)
    

    Use

    Tags

    6xHis-NLS and NLS

    Expression

    Mammalian

    Mutation

    optimized for human codon usage

    Promoter

    CMV and T7

    Available since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-T7-NLS(SV40)-anCas(PDCA)-6xHis-NLS(SV40)

    Plasmid

    #185705

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage anCas(PDCA) with an N-terminal NLS and C-terminal NLS

    Depositor

    Raul Perez-Jimenez

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    anCas(PDCA)
    

    Use

    Tags

    6xHis-NLS and NLS

    Expression

    Mammalian

    Mutation

    optimized for human codon usage

    Promoter

    CMV and T7

    Available since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge