We narrowed to 2,424 results for: puc19

Showing: 1361 - 1380 of 2424 results

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pSEED-mGreenLantern

Plasmid

#219643

Purpose

Permits GoldenGate assembly of donor plasmids (BsmBI) to knock-in mGreenLantern using the SEED/Harvest method

Depositor

Markus Affolter

Article

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

mGreenLantern SEED Cassette

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Blast-miniTurbo

Plasmid

#207681

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-miniTurbo cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pAT15489_dABE8e

Plasmid

#174127

Purpose

plasmid expressing ABE8e with dCas9

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

dABE8e

Use

CRISPR

Tags

Nucleoplasmin NLS and SV40 NLS

Expression

Mammalian

Promoter

EF1-a

Available Since

Nov. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pLoxP-DHFR-mCherry

Plasmid

#70147

Purpose

Expresses a DHFR-mCherry fusion protein and uses for a CRISPR gene deletion

Depositor

David Sibley

Article

Long et al Infect Immun. 2016 Jan 11. doi: 10.1128/

Insert

a selectable dihydrofolate reductase-thymidylate synthase marker

Use

Expression in toxoplasma gondii

Tags

mCherry

Promoter

DHFR 5' UTR

Available Since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only

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qTAG-C-sTagRFP-Puro

Plasmid

#207723

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a sTagRFP-2A-Puro cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pDM053

Plasmid

#216815

Purpose

Aspergillus nidulans codon-adjusted mNeonGreen fluorescent protein, includes linker for N-terminal tagging.

Depositor

Ken Sawin

Article

Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

Insert

mNeonGreen

Tags

RIPGLIN

Expression

Bacterial

Mutation

Aspergillus nidulans codon-adjusted

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pDM071

Plasmid

#216817

Purpose

Aspergillus nidulans codon-adjusted mCardinal fluorescent protein, includes linker for N-terminal tagging.

Depositor

Ken Sawin

Article

Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

Insert

mCardinal

Tags

RIPGLIN

Expression

Bacterial

Mutation

Aspergillus nidulans codon-adjusted

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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qTAG-KO-PGK-Blast

Plasmid

#227265

Purpose

Empty selectable Knock-Out donor cassette. Integrate homology arms to target the N-terminus insertion of a PGK-Blast cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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qTAG-C-miRFP670nano3-Zeo

Plasmid

#207735

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a miRFP670nano3-2A-Zeo cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-N-Zeo-miRFP670nano3

Plasmid

#207702

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-miRFP670nano3 cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pSW068

Plasmid

#140036

Purpose

KO of PCC 11901 fadD gene/expresses codon-optimized 'tesA gene from E. coli from inducible cLac143 promoter

Depositor

Peter Nixon

Article

Wlodarczyk et al Commun Biol. 2020 May 7;3(1):215. doi: 1

Insert

Truncated thioesterase gene

Expression

Bacterial

Promoter

cLac143

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Blast-moxGFP

Plasmid

#207677

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-moxGFP cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pSEED-mCherry

Plasmid

#219657

Purpose

Permits GoldenGate (BsmBI) assembly of donor plasmids to knock-in mCherry using the SEED/Harvest method

Depositor

Markus Affolter

Article

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

sfGFP SEED Cassette

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pDonor14-ShoHELIX-KanR (BO4)

Plasmid

#181795

Purpose

pDonor for ShoHELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence and encoding a kanamycin resistance gene as cargo

Depositor

Benjamin Kleinstiver

Article

Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

KanR, R6K origin of replication

Expression

Bacterial

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Zeo-3xFLAG

Plasmid

#207706

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-3xFLAG cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pVC_CRISPR_Cat

Plasmid

#120368

Purpose

Reporter plasmid for full-site selection assay

Depositor

Jennifer Doudna

Article

Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

Insert

CRISPR_Cat

Use

CRISPR

Available Since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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pAT15482_ABE8e

Plasmid

#174120

Purpose

Expresses ABE8e with an EF1a promoter

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

eABE

Use

CRISPR

Tags

Nucleoplasmin NLS and SV40 NLS

Expression

Mammalian

Promoter

EF1-a

Available Since

Nov. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pAT15068-evoCBE

Plasmid

#163013

Purpose

plasmid expressing evoCBE

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

evoCBE

Use

CRISPR

Tags

3xFLAG and SV40 NLS

Expression

Mammalian

Available Since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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Aurli_150841_GZG

Plasmid

#162563

Purpose

Inactivation of Aurli_150841 (crtIBY) in Aurantiochytrium limacinum MYA-1381 with zeocin resistance driven by native GAPDH promoter/termiator system.

Depositor

Jackie Collier

Article

Rius et al Genome Biol Evol. 2023 Mar 3;15(3):evad0

Insert

shble

Use

Unspecified

Promoter

GAPDH

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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qTAG-C-moxGFP-Blast

Plasmid

#207710

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a moxGFP-2A-Blast cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge