We narrowed to 18,324 results for: try
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Plasmid#128650PurposeEntry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymesDepositorTypeEmpty backboneUseUnspecifiedAvailable SinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only
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CALCOCO1 L140A-mycDDK
Plasmid#137767PurposeCALCOCO1 CLIR mutantDepositorInsertCALCOCO1 L140A
TagsmycDDKExpressionMammalianMutationL140APromoterCMVAvailable SinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
CALCOCO1 R12H-mycDDK
Plasmid#137770PurposeCALCOCO1 R12H mutantDepositorInsertCALCOCO1 R12H
TagsmycDDKExpressionMammalianMutationR12HPromoterCMVAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 LC3B2 no-stop
Plasmid#123207PurposeGateway entry clone encoding human MAP1LC3B2 lacking stop codon, suitable for C-terminal taggingDepositorInsertMicrotubule-associated proteins 1A/1B light chain 3 beta 2 (MAP1LC3B2 Human)
UseGateway entry vector / entry cloneMutationStop codon removedAvailable SinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRPa-c6MYCn
Plasmid#69242PurposeTet-inducible RRNA promoter driven plasmid for expression of N or C-terminally c-Myc tagged protein from a specific RRNA spacer locus in T. brucei.DepositorTypeEmpty backboneUseExpression in trypanosoma bruceiTagscMycPromoterRRNA promoterAvailable SinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRP-c6MYCn
Plasmid#69243PurposeTet-inducible RRNA promoter driven plasmid for inducible expression of N- or C-terminally c-Myc tagged protein from a random RRNA spacer locus in T. brucei.DepositorTypeEmpty backboneUseExpression in trypanosoma bruceiTagscMycPromoterRRNA promoterAvailable SinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-tdTomato-WPRE
Plasmid#133786PurposeCre-dependent tdTomato fluorescent reporter for cell labelingDepositorInsertpAAV-EF1a-DIO-tdTomato-WPRE
UseAAV and Cre/LoxExpressionMammalianAvailable SinceJune 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1(7FTrp)RS
Plasmid#177310PurposetRNA synthetase/tRNA pair for the in vivo incorporation of 7-Fluoro-L-Tryptophan (7FTrp) into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
ExpressionBacterialPromoterT7Available SinceJan. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19-U6-LwaCas13acrRNA-BsmBIcassette (LTH151)
Plasmid#171129PurposeLwaCas13a crRNA entry vector for pU6 expression (need to clone in spacer into BsmBI sites)DepositorInsertU6 promoter entry plasmid for LwaCas13a crRNA cloning
UseCRISPRExpressionMammalianPromoterU6Available SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTrex-Neo-tdTomato
Plasmid#47975PurposeTrasgenically expresses tandem tdtomato fluorescent protein in T. cruziDepositorInserttdTomato
UseTrypanasoma cruzi expressionPromoterT. cruzi rRNAAvailable SinceApril 10, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
MC177VSG221RNAi
Plasmid#59730PurposeFor use in trypanosome cell lines already expressing T7 polymerase and Tet-repressor. Tetracycline induction results in the production of dsRNA targeting VSG221.DepositorInsertVSG221 RNAi fragment
UseRNAiPromoteropposing T7 promotersAvailable SinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 LC3C WT
Plasmid#123078PurposeGateway entry clone encoding human MAP1LC3C wild-typeDepositorInsertMicrotubule-associated proteins 1A/1B light chain 3C (MAP1LC3C Human)
UseGateway entry vector / entry cloneAvailable SinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMSP1D1R1
Plasmid#97323PurposeNanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional R residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.DepositorInsertMSP1D1R1
Tags6xHis - TEVExpressionBacterialMutationAddition of R residue after T25PromoterT7Available SinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 GABARAP WT
Plasmid#123080PurposeGateway entry clone encoding human GABARAP wild-typeDepositorInsertGamma-aminobutyric acid receptor-associated protein (GABARAP Human)
UseGateway entry vector / entry cloneAvailable SinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMSP1D1G1
Plasmid#97325PurposeNanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional G residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.DepositorInsertMSP1D1G1
Tags6xHis - TEVExpressionBacterialMutationAddition of G residue after T25PromoterT7Available SinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMSP1D1T1
Plasmid#97327PurposeNanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional T residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.DepositorInsertMSP1D1T1
Tags6xHis - TEVExpressionBacterialMutationAddition of T residue after T25PromoterT7Available SinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
592 p3e-EST etv2 3’UTR
Plasmid#49003PurposeZebrafish EST annotated length etv2 3' UTR in gateway 3' Entry vectorDepositorInsertets variant gene 2 (etsrp Zebrafish)
UseGateway 3' entryMutationonly the 3' UTRPromoter3' gateway entry vectorAvailable SinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pDONR223 SARS-CoV-1 NSP6_stop
Plasmid#168548PurposeGateway-compatible Entry vectorDepositorInsertNSP6 (ORF1ab )
UseOtherAvailable SinceJan. 20, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223 MERS-CoV NSP6_stop
Plasmid#168633PurposeGateway-compatible Entry vectorDepositorInsertNSP6 (orf1ab )
UseOtherAvailable SinceJuly 5, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits