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Plasmid#70101Purposean RNA spike-in with similar GC content to S. cerevisiae mRNAs, for in vivo measurement of RNA kineticsDepositorInsert1200bp insert from B. subtillis
UseIn vitro transcriptionTagsExpressionMutationPromoterSP6Available sinceNov. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAG426-GAL-3xFLAG-PolyS
Plasmid#181728PurposeGalactose inducible expression of 3xFLAG-PolySDepositorInsert3xFLAG-PolyS
UseTags3xFLAGExpressionYeastMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMAL-c5X-poly(alanylglycine)
Plasmid#67003PurposeExpresses malE-poly(alanylglycine) in E. coliDepositorInsertmalE-poly(alanylglycine)
UseTagsHis6 and malE-Factor XaExpressionBacterialMutationPromotertacAvailable sinceJan. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMAL-c5X-poly-EAK9
Plasmid#67010PurposeExpresses malE-poly-EAK9 in E. coliDepositorInsertmalE-poly-EAK9
UseTagsHis6 and malE-Factor XaExpressionBacterialMutationPromotertacAvailable sinceJan. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
HO-pGAL-poly-KanMX4-HO
Plasmid#51664Purposeyeast plasmid for integration of target sequence at HO locus, Kan selection, pGAL inducible promoterDepositorTypeEmpty backboneUseYeast integrationTagsExpressionYeastMutationPromoterAvailable sinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
HO-pMET-poly-KanMX4-HO
Plasmid#51665Purposeyeast plasmid for integration of target sequence at HO locus, Kan selection, pMET inducible promoterDepositorTypeEmpty backboneUseYeast integrationTagsExpressionYeastMutationPromoterAvailable sinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
attB-mCh-Dnmt3b1-Poly(A)-PuroR
Plasmid#65556PurposePlasmid for Bxb1-mediated recombination of the mCh-Dnmt3b1 cDNA into a MIN-tagged locus using Puromycin selectionDepositorInsertDnmt3b (Dnmt3b Mouse)
UseMouse Targeting; Bxb1TagsmCherryExpressionMammalianMutationisoform 1PromoterAvailable sinceAug. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
attB-mCh-Dnmt3b6-Poly(A)-NeoR
Plasmid#65554PurposePlasmid for Bxb1-mediated recombination of the mCh-Dnmt3b6 cDNA into a MIN-tagged locus using Neomycin selectionDepositorInsertDnmt3b (Dnmt3b Mouse)
UseMouse Targeting; Bxb1TagsmCherryExpressionMammalianMutationisoform 6PromoterAvailable sinceAug. 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAWH-Rluc-let-7-Poly(A)
Plasmid#50556PurposeExpression of luciferase reporter mRNA in S2 cellDepositorInsertLuciferase-let7-Poly(A)
UseLuciferaseTagsExpressionInsectMutationPromoterActin5CAvailable sinceJan. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
attB-mCh-Dnmt3b1-Poly(A)-NeoR
Plasmid#65553PurposePlasmid for Bxb1-mediated recombination of the mCh-Dnmt3b1 cDNA into a MIN-tagged locus using Neomycin selectionDepositorInsertDnmt3b (Dnmt3b Mouse)
UseMouse Targeting; Bxb1TagsmCherryExpressionMammalianMutationisoform 1PromoterAvailable sinceAug. 31, 2015AvailabilityAcademic Institutions and Nonprofits only