We narrowed to 6,513 results for: GCT

1. pcDNA5/FRT/TO/intron/FlagCter

   Plasmid

   #113548

   Purpose

   Mammalian expression plasmid that can be used with the Flp-In T-REx system.

   Depositor

   Blerta Xhemalce

   Article

   Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

   Insert

   Flag
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV-U6-sgCTG-CMV-GFP

   Plasmid

   #216734

   Purpose

   AAV plasmid containing sgCTG (target sequence: (CUG)6) driven by a U6 promoter and eGFP under the control of the CMV promoter. Used for in vivo experiments.

   Depositor

   Vincent Dion

   Article

   Alvaro et al bioRxiv

   Insert

   sgCTG
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and CMV

   Available since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pPiggyBacATCC LacZ GCTT loxAttP

   Plasmid

   #173496

   Purpose

   piggyBac insect transgenesis destination vector for Golden Gate Cloning assemblies

   Depositor

   Eric Marois

   Article

   Lutrat et al Commun Biol. 2022 Mar 7;5(1):210. doi: 1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pENTR R4 ATCC LacZ GCTT

   Plasmid

   #173668

   Purpose

   Destination vector for Golden Gate Cloning assemblies, blue-white screening

   Depositor

   Eric Marois

   Article

   Green et al Elife. 2023 Dec 5;12:e93142. doi: 10.755

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Insect

   Mutation

   Promoter

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA5/FRT/TO/intron/FlagCter

   Plasmid

   #113548

   Purpose

   Mammalian expression plasmid that can be used with the Flp-In T-REx system.

   Depositor

   Blerta Xhemalce

   Article

   Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

   Insert

   Flag
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-U6-sgCTG-CMV-GFP

   Plasmid

   #216734

   Purpose

   AAV plasmid containing sgCTG (target sequence: (CUG)6) driven by a U6 promoter and eGFP under the control of the CMV promoter. Used for in vivo experiments.

   Depositor

   Vincent Dion

   Article

   Alvaro et al bioRxiv

   Insert

   sgCTG
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and CMV

   Available since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLV(gRNA)-CMV-eGFP-U6(sgCTG)

   Plasmid

   #216732

   Purpose

   Contains a eGFP gene along with a U6 promoter driving the sgCTG (target sequence: (CUG)6). Used with the HD iPSC-derived astrocytes

   Depositor

   Vincent Dion

   Article

   Alvaro et al bioRxiv

   Insert

   sgCTG lentiviral guide RNA with eGFP tag
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pKSB-631 U6-sgRNA3/4 AACA GCTT

   Plasmid

   #173675

   Purpose

   To clone and express a gRNA in Anopheles

   Depositor

   Eric Marois

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pU6-sgCtrl_EF1a-Puro-T2A-BFP

   Plasmid

   #195500

   Purpose

   Lentiviral BFP expression vector bearing a sgRNA targeting a randomized human TSS sequence

   Depositor

   Alexander Meissner

   Article

   Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

   Insert

   sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   BFP, Puro, and T2A

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLV(gRNA)-CMV-eGFP-U6(sgCTG)

    Plasmid

    #216732

    Purpose

    Contains a eGFP gene along with a U6 promoter driving the sgCTG (target sequence: (CUG)6). Used with the HD iPSC-derived astrocytes

    Depositor

    Vincent Dion

    Article

    Alvaro et al bioRxiv

    Insert

    sgCTG lentiviral guide RNA with eGFP tag
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pKSB-631 U6-sgRNA3/4 AACA GCTT

    Plasmid

    #173675

    Purpose

    To clone and express a gRNA in Anopheles

    Depositor

    Eric Marois

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pU6-sgCtrl_EF1a-Puro-T2A-BFP

    Plasmid

    #195500

    Purpose

    Lentiviral BFP expression vector bearing a sgRNA targeting a randomized human TSS sequence

    Depositor

    Alexander Meissner

    Article

    Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

    Insert

    sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    BFP, Puro, and T2A

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLenti-U6-sgCTG-CAG-Cas9D10A nickase-Blast

    Plasmid

    #216733

    Purpose

    Expresses SpCas9-D10A nickase under a CAG promoter together with a blasticidin resistance gene, along with a U6 promoter driving the sgCTG (target sequence: (CUG)6). Suitable for lentivirus packaging.

    Depositor

    Vincent Dion

    Article

    Alvaro et al bioRxiv

    Insert

    Cas9-D10A nickase and sgCTG
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    U6 and CAG

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLenti-U6-sgCTG-CAG-Cas9D10A nickase-Blast

    Plasmid

    #216733

    Purpose

    Expresses SpCas9-D10A nickase under a CAG promoter together with a blasticidin resistance gene, along with a U6 promoter driving the sgCTG (target sequence: (CUG)6). Suitable for lentivirus packaging.

    Depositor

    Vincent Dion

    Article

    Alvaro et al bioRxiv

    Insert

    Cas9-D10A nickase and sgCTG
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    U6 and CAG

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTwist-SARS-CoV-2 Spike-deltaC18 T376A (GCT)

    Plasmid

    #212492

    Purpose

    Encodes SARS-CoV-2 spike T376A for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike T376A (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T376A

    Promoter

    CAG

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTwist-SARS-CoV-2 Spike-deltaC18 D80A (GCT)

    Plasmid

    #212344

    Purpose

    Encodes SARS-CoV-2 spike D80A for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike D80A (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D80A

    Promoter

    CAG

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTwist-SARS-CoV-2 Spike-deltaC18 T376A (GCT)

    Plasmid

    #212492

    Purpose

    Encodes SARS-CoV-2 spike T376A for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike T376A (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T376A

    Promoter

    CAG

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTwist-SARS-CoV-2 Spike-deltaC18 D80A (GCT)

    Plasmid

    #212344

    Purpose

    Encodes SARS-CoV-2 spike D80A for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike D80A (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D80A

    Promoter

    CAG

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pUC57-ITR2-H1-sgCTG-SlugD10A-3XFLAG-SV40 NLS-ITR2

    Plasmid

    #222857

    Purpose

    This plasmid codes for the Slug-KH nickase with a sg(CTG)6

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Insert

    Slug-KHD10A+sgCTG
    

    Use

    CRISPR

    Tags

    3X FLAG, SV40 NLS, ITR2 and ITR2

    Expression

    Mammalian

    Mutation

    KH variant and D10A mutation

    Promoter

    H1 and eCMV

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUC57-ITR2-H1-sgCTG-SlugD10A-3XFLAG-SV40 NLS-ITR2

    Plasmid

    #222857

    Purpose

    This plasmid codes for the Slug-KH nickase with a sg(CTG)6

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Insert

    Slug-KHD10A+sgCTG
    

    Use

    CRISPR

    Tags

    3X FLAG, SV40 NLS, ITR2 and ITR2

    Expression

    Mammalian

    Mutation

    KH variant and D10A mutation

    Promoter

    H1 and eCMV

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge