We narrowed to 180 results for: PTN

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pTNT-pT7-tetR-sfGFP

Plasmid

#140868

Purpose

T7 promoter driven synthesis of C-terminal fusion of sfGFP to TetR repressor (TetR-sfGFP)

Depositor

Neal Devaraj

Article

Niederholtmeyer et al Nat Commun. 2018 Nov 28;9(1):5027. doi:

Insert

TetR-sfGFP expressed with T7 promoter

Use

Synthetic Biology

Tags

sfGFP

Expression

Bacterial

Promoter

T7

Available Since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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pMS10: pTns(AvCAST)_ΔTnsD

Plasmid

#168143

Purpose

Inducible expression of AvCAST TnsA, TnsB, TnsC and TniQ proteins.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Insert

AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE-eGFP-OPTN S513A

Plasmid

#175764

Purpose

Lentiviral vector expressing N-terminally tagged eGFP-OPTN S513A

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

OPTN (OPTN Human)

Use

Lentiviral

Mutation

S513A

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pMS26: pTns(PmcCAST)_ΔTniQ

Plasmid

#168159

Purpose

Inducible expression of PmcCAST TnsAB, TnsC and TnsD proteins.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Insert

PmcCAST Tns proteins (TnsAB, TnsC and TnsD)

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pMS27: pTns(PmcCAST)_ΔTnsD

Plasmid

#168160

Purpose

Inducible expression of PmcCAST TnsAB, TnsC and TniQ proteins.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

PmcCAST Tns proteins (TnsAB and TnsC)
PmcTniQ

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE-FKBP-GFP-OPTN

Plasmid

#208866

Purpose

Stably express GFP-tagged OPTN for CID induced mitophagy

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

OPTN (OPTN Human)

Tags

FKBP-GFP

Expression

Mammalian

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET-Duet_OPTN-thrombin-GST

Plasmid

#216843

Purpose

For expression in E. coli of OPTN (Internal ref. SMC1789)

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

Optineurin (OPTN Human)

Tags

GST

Expression

Bacterial

Promoter

T7 promoter

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-eGFP-OPTN D474N

Plasmid

#175762

Purpose

Lentiviral vector expressing N-terminally tagged eGFP-OPTN D474N

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

OPTN (OPTN Human)

Use

Lentiviral

Mutation

D474N

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pMS9: pTns(AvCAST)_ΔTniQ

Plasmid

#168142

Purpose

Inducible expression of AvCAST TnsA, TnsB, TnsC and TnsD proteins.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

AvCAST Tns proteins (TnsA, TnsB and TnsC)
AvTnsD

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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lentiSAMv2 OPTN TSS-guide1

Plasmid

#118196

Purpose

CRISPR-mediated activation of OPTN. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

dCas9-VP64-2A-BlastR

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a core and U6

Available Since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

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lentiSAMv2 OPTN TSS-guide3

Plasmid

#118198

Purpose

CRISPR-mediated activation of OPTN. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

dCas9-VP64-2A-BlastR

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a core and U6

Available Since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

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lentiSAMv2 OPTN TSS-guide2

Plasmid

#118197

Purpose

CRISPR-mediated activation of OPTN. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

dCas9-VP64-2A-BlastR

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a core and U6

Available Since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Tornado-F30-Broccoli-aptNFkB#5

Plasmid

#124362

Purpose

Overexpression of bifunctional circular RNA (Broccoli fluorescence and NFkB binding)

Depositor

Samie Jaffrey

Article

Litke et al Nat Biotechnol. 2019 Apr 8. pii: 10.1038

Insert

Tornado-F30-Broccoli-aptNFkB#5

Expression

Mammalian

Promoter

U6+27

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pBMN mCherry-OPTN(F178A/D474N)

Plasmid

#119684

Purpose

For mammalian expression of LIR/UBD double mutant OPTN

Depositor

Michael Lazarou

Article

Padman et al Nat Commun. 2019 Jan 24;10(1):408. doi:

Insert

OPTN (OPTN Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Mutation

F178A/D474N

Available Since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-FKBP-GFP-OPTN (2-119)

Plasmid

#208867

Purpose

Stably express GFP-tagged OPTN (2-119aa) for CID induced mitophagy

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

OPTN (OPTN Human)

Tags

FKBP-GFP

Expression

Mammalian

Mutation

aa 2-119 only

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pCRII-Topo Ptn in situ probe

Plasmid

#45632

Depositor

Jeffrey Macklis

Article

Molyneaux et al J Neurosci. 2009 Sep 30;29(39):12343-54.

Insert

Ptn in situ probe (Ptn Mouse)

Use

In situ

Mutation

fragment contains bp# 1241-1830 of NM_008973

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
pMS28: pTns(PmcCAST)_ΔTniQ,ΔTnsD

Plasmid

#168161

Purpose

Inducible expression of PmcCAST TnsAB and TnsC proteins.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Insert

PmcCAST Tns proteins (TnsAB and TnsC)

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pET-Duet1-6xHis-TEV-OPTN

Plasmid

#190192

Purpose

Plasmid for the expression of 6xHis-TEV-OPTN. Internal reference: SMC397

Depositor

Sascha Martens

Article

Zaffagnini et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

Insert

optineurin (OPTN Human)

Tags

6xHis and TEV

Expression

Bacterial

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-eGFP-OPTN S473A 513A

Plasmid

#175763

Purpose

Lentiviral vector expressing N-terminally tagged eGFP-OPTN S473A 513A

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

OPTN (OPTN Human)

Use

Lentiviral

Mutation

S473A S513A

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pMS11: pTns(AvCAST)_ΔTniQ,ΔTnsD

Plasmid

#168144

Purpose

Inducible expression of AvCAST TnsA, TnsB and TnsC proteins.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Insert

AvCAST Tns proteins (TnsA, TnsB and TnsC)

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge