Skip to main content
Addgene
Showing: 121 - 140 of 21750 results

  6. pEM047 [T7-assisted CROPseq sgRNA expression vector]

    Plasmid
    #224899
    Purpose
    Lentiviral CROP-seq vector with lineage barcode cassette and T7 promoter for ID amplification from cDNA
    Depositor
    Thomas Norman
    Article
    Metzner et al bioRxiv [Preprint]. 2024 Jul 27:2024.07.
    Inserts
    eGFP
    LacZ fragment (removed by digestion with BstXI/BlpI for sgRNA cloning)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available since
    Aug. 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    224899-plasmid-map-sequence-id-443624

    Enlarge

  8. XLone-eGFP NLS (no drug resistant gene)

    Plasmid
    #140032
    Purpose
    Tunable and temporal expression control of eGFP (or gene of interest) with constitutive nuclear eGFP
    Depositor
    Xiaoping Bao
    Insert
    Enhanced Green Fluorescent Protein
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G
    Available since
    May 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    140032-plasmid-map-sequence-id-275878

    Enlarge

  13. pCDNA5/FRT/TO–γ-tubulinWT-RNAi-resistant-GFP

    Plasmid
    #205603
    Purpose
    Tet-inducible expression of WT gamma-tubulin in human cells, harbors silent mutations in shRNA target site, FLP-In mediated insertion
    Depositor
    Tarun Kapoor
    Article
    Berman et al J Cell Biol. 2023 Mar 6;222(3):e20220410
    Insert
    γ-tubulinWT-RNAi-resistant-GFP
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    G2228A, C2231T, C2237T, G2240A
    Promoter
    Available since
    Aug. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    205603-plasmid-map-sequence-id-405874

    Enlarge

  14. pCDNA5/FRT/TO–γ-tubulinΔGTP-RNAi-resistant-GFP

    Plasmid
    #205604
    Purpose
    Tet-inducible expression of N229A gamma-tubulin in human cells, harbors silent mutations in shRNA target site, FLP-In mediated insertion
    Depositor
    Tarun Kapoor
    Article
    Berman et al J Cell Biol. 2023 Mar 6;222(3):e20220410
    Insert
    γ-tubulinΔGTP-RNAi-resistant-GFP
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    G2228A, C2231T, C2237T, G2240A, N229A
    Promoter
    Available since
    Aug. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    205604-plasmid-map-sequence-id-405873

    Enlarge

  17. pEN_TT 3XFLAG-S37A-GATA6-3XAU1_RNAi resistant

    Plasmid
    #72924
    Purpose
    Gateway entry vector for an inducible N-terminally 3XFLAG-tagged and C-terminally AU1-tagged, RNAi resistant human S37A-GATA6
    Depositor
    Kevin Janes
    Article
    Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:
    Insert
    GATA Binding Protein 6 (GATA6 Human)
    Use
    Entry vector for gateway cloning
    Tags
    3XAU1 and 3XFLAG
    Expression
    Bacterial
    Mutation
    Serine#37 changed to Alanine;Silent mutations mad…
    Promoter
    TRE tight
    Available since
    July 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    72924-plasmid-map-sequence-id-138470

    Enlarge

  18. pSLIKneo 3XFLAG-S37A-GATA6-3XAU1_RNAi resistant

    Plasmid
    #72617
    Purpose
    Expresses an inducible and RNAi resistant, N-terminally 3X-FLAG tagged, C-terminally 3X-AU1 tagged S37AGATA6
    Depositor
    Kevin Janes
    Article
    Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:
    Insert
    GATA Binding Protein 6 (GATA6 Human)
    Use
    Lentiviral
    Tags
    3XAU1 and 3XFLAG
    Expression
    Mammalian
    Mutation
    Serine#37 changed to Alanine;Silent mutations mad…
    Promoter
    TRE tight
    Available since
    July 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    72617-plasmid-map-sequence-id-138346

    Enlarge

Showing: 121 - 140 of 21750 results