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Plasmid#113857PurposeFor Doxycycline-inducible expression of Humanized Beta synaptase gene, with red fluorescent protein gene E2-Crimson-P2A-Humanized Beta synaptase geneDepositorInsertHumanized Beta
UseLentiviralTagsP2A/CrimsonExpressionMutation"humanized" Beta genePromoterAvailable sinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-EF1α1.1-FLPX-rc [ChrimsonR-GFP]
Plasmid#128588PurposeAAV-mediated expression of ChrimsonR-GFP under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner.DepositorInsertChrimsonR-GFP
UseAAVTagsGFPExpressionMammalianMutationChrimson K176R mutantPromoterEF1α (1.1 kb short version)Available sinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40 (AAV9)
Viral Prep#124603-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40 (#124603). In addition to the viral particles, you will also receive purified pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40 plasmid DNA. EF1a-driven, Cre-dependent expression of ChrimsonR fused to mRuby2 fluorophore and targeted to neuronal soma for optogenetic activation. These AAV preparations are suitable purity for injection into animals.DepositorPromoterTagsmRuby2 (Cre-dependent)Available sinceSept. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pETSXM2-pLacI-mtrC
Plasmid#174615PurposeBacterial expression of mtrC from Shewanella oneidensis MR-1.DepositorInsertMtrC
UseSynthetic Biology; Shuttle vector in e. coli and …TagsExpressionBacterialMutationPromoterpLacIAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETSXM2-pLacI-mtrCAB
Plasmid#174618PurposeBacterial expression of gene cluster mtrCAB from Shewanella oneidensis MR-1.DepositorInsertmtrCAB
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpLacIAvailable sinceNov. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHdzCas9-KRAB
Plasmid#92341PurposeExpression dzCas9-KRAB in microalgae. CRISPR/Cas based plant genome editing and gene regulation, Hyg resistanceDepositorTypeEmpty backboneUseCRISPR; Plant expressionTagsKRABExpressionPlantMutationPromoterCaMV35SAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pIKU801-CrPEPC1
Plasmid#92342PurposeExpresses sgRNA for targeting CrPEPC1 gene, paromomycin resistanceDepositorInsertsgRNA-CrPEPC1
UseCRISPRTagsExpressionPlantMutationPromoterAtU6-26Available sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pETSXM2-pLacI-GFP
Plasmid#174617PurposeBacterial expression of GFP.DepositorInsertGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpLacIAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSUI-SynP-CAS1sg
Plasmid#154341PurposeExpression of the sgRNA targeting to the E. coli can geneDepositorInsertCAS1 sgRNA
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSUI-SynP-CAS2sg
Plasmid#154342PurposeExpression of the sgRNA targeting to the E. coli can geneDepositorInsertCAS2 sgRNA
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSUI-SynP-CANTsg
Plasmid#154343PurposeExpression of the sgRNA targeting to the E. coli can geneDepositorInsertCANT sgRNA
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSM-dCas9::Ap
Plasmid#154307PurposeShuttle vector for expression dCas9, mobilization cluster, expresses the tracrRNA and a CRISPR array designed for the easy cloning of new spacers.DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSUI-SynP-sfGFP
Plasmid#154306PurposeExpression of sfGFP under Synthetic promoter (SynP) and cloning of the sgRNA with simple screening of positive clone by fluorescenceDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSUI-SynP-CAPsg
Plasmid#154344PurposeExpression of the sgRNA targeting to the E. coli can geneDepositorInsertCAP sgRNA
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-jGCaMP8s-P2A-ChrimsonR-ST
Plasmid#174007PurposeCre dependent co-expression of jGCaMP8s and soma-targeted ChrimsonR under the control of an hSyn promoter. jGCaMP8s and ChrimsonR are separated by cleavable peptide sequence P2A.DepositorHas ServiceAAV9InsertjGCaMP8s-P2A-ChrimsonR-ST
UseAAVTags6xHis and soma targeting motifExpressionMutationPromoterhSynAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
H516 SONIC KrasG12D-IRES-luciferase donor
Plasmid#138178PurposeCRISPR SONIC: Kras G12D luciferase donor plasmid.DepositorInsertKrasG12D (Kras Mouse)
UseNhej donorTagsExpressionMutationPromoterAvailable sinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-pOst1-pro-alphaf(MUT1)-E2-Crimson
Plasmid#117662PurposeTest intracellular localisation of E2-Crimson and study the secretion efficiencyDepositorInsertpOst1-pro-af(MUT1)-E2-Crimson
UseTagsExpressionYeastMutationThis plasmid encodes the fluorescent protein E2-C…PromoterpAOX1Available sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pALS343 (pGEX-2TP-cTevHis-Gibson/MCS)
Plasmid#139788PurposeVector for recombinant protein expression in E. coli; PreScission-cleavable N-terminal GST and TEV-cleavable C-terminal 6xHisDepositorTypeEmpty backboneUseTags6xHis (cleavable by TEV protease) and GST (cleava…ExpressionBacterialMutationPromoterAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
fru-T2A-CsChrimson-tdTomato HDR plasmid
Plasmid#141100PurposePlasmid for CRISPR-generated knock-in line that expresses optogenetic activator CsChrimson-tdTomato by knocking-in fragment into the coding sequence of the endogenous Aedes aegypti fru geneDepositorInsertfru-left-HDR-arm; T2A-CsChrimson-tdTomato-SV40; 3xP3-EYFP-SV40; fru-right-HDR-arm
UseDonor templateTagsExpressionMutationPromoterAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
YIplac204-pOst1-APVNTT-E2-Crimson-FKBP(LV)(C22V)
Plasmid#140155PurposeExpresses a regulatable secretory cargo for yeast at the TRP1 locusDepositorInsertpOst1-APVNTT-E2-Crimson-FKBP(LV)(C22V)
UseTagsExpressionYeastMutationPromoterTPI1Available sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only