Addgene

We narrowed to 3,545 results for: apoe

Showing: 121 - 140 of 3545 results

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pET-APOBEC1-YTHmut

Plasmid

#200159

Purpose

For bacterial expression of the APOBEC1-YTHmut fusion protein for protein purification

Depositor

Kate Meyer

Article

Zhu et al Front Cell Dev Biol. 2022 Apr 27;10:8882

Insert

APOBEC1-YTHmut

Use

Tags

6xHis, HA, and MBP

Expression

Bacterial

Mutation

Codon optimized for E coli expression

Promoter

LacZ

Availability

Academic Institutions and Nonprofits only

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AAV-EGFP-APOBEC1

Plasmid

#209321

Purpose

AAV packaging vector containing a EGFP-P2A expression cassette, APOBEC1 expression.

Depositor

Magdalena Koziol

Article

Feng et al bioRxiv 2023.12.06.570314

Insert

APOBEC1-HA

Use

AAV

Tags

HA

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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Ca-LiPPI-CLAPon1.0 (APEX)

Plasmid

#213408

Purpose

Light-gated peroxidase (APEX) reporter for detecting calcium-dependent CaM and MK2 interaction (component 1)

Depositor

Wenjing Wang

Article

Zhou et al J Am Chem Soc. 2022 Dec 21;144(50):22933

Insert

MK2-E5-truncated hlov-TEVcs-APEX(201-250)-K5-HA-HA-APEX(2-200)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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Ca-LiPPI-CLAPon1.0 (TEVp)

Plasmid

#213409

Purpose

Light-gated peroxidase (APEX) reporter for detecting calcium-dependent CaM and MK2 interaction (component 2)

Depositor

Wenjing Wang

Article

Zhou et al J Am Chem Soc. 2022 Dec 21;144(50):22933

Insert

CaM-V5-TEVp

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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hCRISPRi-v2, Cancer and Apoptosis (h2), top 5 sgRNAs/gene

Pooled Library

#83972

Purpose

Human CRISPRi Pooled Library targeting cancer and apoptosis genes

Depositor

Jonathan Weissman

Article

Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

Availability

Academic Institutions and Nonprofits only
hCRISPRa-v2, Cancer and Apoptosis (h2), top 5 sgRNAs/gene

Pooled Library

#83981

Purpose

Human CRISPRa Pooled Library targeting cancer and apoptosis genes

Depositor

Jonathan Weissman

Article

Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

Availability

Academic Institutions and Nonprofits only
mCRISPRi-v2, Cancer and Apoptosis (m2), top 5 sgRNAs/gene

Pooled Library

#83990

Purpose

Mouse CRISPRi Pooled Library targeting cancer and apoptosis genes

Depositor

Jonathan Weissman

Article

Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

Availability

Academic Institutions and Nonprofits only
mCRISPRa-v2, Cancer and Apoptosis (m2), top 5 sgRNAs/gene

Pooled Library

#83999

Purpose

Mouse CRISPRa Pooled Library targeting cancer and apoptosis genes

Depositor

Jonathan Weissman

Article

Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

Availability

Academic Institutions and Nonprofits only
Venus-tagged Apollo-NADP+

Plasmid

#71797

Purpose

Expresses Venus-tagged Apollo-NADP+ in mammalian cells as a reporter for cytoplasmic NADP+

Depositor

Jonathan Rocheleau

Article

Cameron et al Nat Methods. 2016 Feb 15. doi: 10.1038/n

Insert

Apollo-NADP+

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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Cerulean-tagged Apollo-NADP+

Plasmid

#71800

Purpose

Expresses Cerulean-tagged Apollo-NADP+ in mammalian cells as a reporter for cytoplasmic NADP+

Depositor

Jonathan Rocheleau

Article

Cameron et al Nat Methods. 2016 Feb 15. doi: 10.1038/n

Insert

Apollo-NADP+

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pTC-ApoE-Tet-YAP

Plasmid

#85580

Purpose

Sleeping beauty transposon for hydrodynamic tail vein injection for CreER expression and tetracyclin-dependent YAP expression (ApoE.HCR.hAAT promoter) in mouse liver

Depositor

Ursula Ehmer

Article

Hubner et al J Gene Med. 2016 Dec 23. doi: 10.1002/jg

Inserts

CreER
YAP

Use

Cre/Lox

Tags

Flag (2x)

Expression

Mammalian

Mutation

Promoter

ApoE.HCR.hAAT and TRE

Availability

Academic Institutions and Nonprofits only

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1184_pAAV-U6-ApoB-gRNA2-CB-EmGFP

Plasmid

#89062

Purpose

AAV-gRNA targeting the murine Apob gene

Depositor

William Lagor

Article

Jarrett et al Sci Rep. 2017 Mar 16;7:44624. doi: 10.10

Insert

EmGFP

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Chicken beta actin with partial CMV enhancer

Availability

Academic Institutions and Nonprofits only

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pEGFP mCherry-ApoB-EGFP

Plasmid

#112859

Purpose

Plasmid bearing the editing target of APOBEC1 fused between the mCherry cds and the EGFP one. In presence of APOBEC1, the CAA codon in ApoB is edited to a Stop codon (UAA), leading to loss of EGFP

Depositor

Silvestro Conticello

Article

Severi et al RNA Biol. 2015;12(4):389-97. doi: 10.108

Insert

mCherry-apoB-EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter

Availability

Academic Institutions and Nonprofits only

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pT3TS-TAL-apobb.1-PR1-LA

Plasmid

#128693

Purpose

Left arm of TALEN pair 1 for cleavage of apobb.1 STOP locus

Depositor

Steven Farber

Article

Thierer et al Nat Commun. 2019 Jul 31;10(1):3426. doi:

Insert

TALEN

Use

TALEN

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pT3TS-TAL-apobb.1-PR1-RA

Plasmid

#128694

Purpose

Right arm of TALEN pair 1 for cleavage of apobb.1 STOP locus

Depositor

Steven Farber

Article

Thierer et al Nat Commun. 2019 Jul 31;10(1):3426. doi:

Insert

TALEN

Use

TALEN

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pT3TS-TAL-apobb.1-PR2-LA

Plasmid

#128695

Purpose

Left arm of TALEN pair 2 for cleavage of apobb.1 STOP locus

Depositor

Steven Farber

Article

Thierer et al Nat Commun. 2019 Jul 31;10(1):3426. doi:

Insert

TALEN

Use

TALEN

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pT3TS-TAL-apobb.1-PR2-RA

Plasmid

#128696

Purpose

Right arm of TALEN pair 2 for cleavage of apobb.1 STOP locus

Depositor

Steven Farber

Article

Thierer et al Nat Commun. 2019 Jul 31;10(1):3426. doi:

Insert

TALEN

Use

TALEN

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-mCherry-ApoB-TagBFP

Plasmid

#129383

Purpose

Plasmid bearing the editing target of APOBEC1 fused between the mCherry and the Tag-BFP one. In presence of APOBEC1, the CAA codon in ApoB is edited to a Stop codon (UAA), leading to loss of Tag-BFP

Depositor

Silvestro Conticello

Article

Chieca et al Methods Mol Biol. 2021;2181:69-81. doi:

Insert

mCherry-ApoB-Tag-BFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter

Availability

Academic Institutions and Nonprofits only

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pCMV_UdgX-APOBEC1-UdgX-nCas9

Plasmid

#163555

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

UdgX-APOBEC1-UdgX-nCas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

nCas9 (D10A)

Promoter

Availability

Academic Institutions and Nonprofits only

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pCMV_UdgX-APOBEC1-UdgX-HF-nCas9

Plasmid

#163559

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

UdgX-APOBEC1-UdgX-HF-nCas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

nCas9 (D10A)HF (N497A, R661A, Q695A, Q926A)

Promoter

Availability

Academic Institutions and Nonprofits only

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