Skip to main content
Addgene
Showing: 121 - 140 of 537 results
  1. pcDNA-GFP-EBA175-Flag(P1424D/Y1426S)

    Plasmid
    #155005
    Purpose
    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424D/Y1426S variant (residues 1284-1462) from pcDNA3.1
    Depositor
    Insert
    PfEBA175
    Use
    Tags
    Flag, GFP, and signal peptide (residues 1 to 32) …
    Expression
    Mammalian
    Mutation
    P1424D/Y1426S; insert codes for EBA175 residues 1…
    Promoter
    CMV
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
  2. pRS426-TUB1-internal6xHis - human TUBA1a Cterminal tail

    Plasmid
    #60392
    Purpose
    Expression of chimeric yeast alpha tubulin (TUB1) - internal 6xHis with human alpha tubulin Cterminal tail (TUBA1a) using a GAL promoter
    Depositor
    Insert
    TUB1 (TUB1 Budding Yeast, Synthetic)
    Use
    Tags
    Expression
    Yeast
    Mutation
    internal 6xHis (see supplement figure 4), amino a…
    Promoter
    GAL
    Available since
    Jan. 26, 2015
    Availability
    Academic Institutions and Nonprofits only
  3. pcDNA-GFP-EBA175-Flag(E1418K/P1424Q/Y1426S)

    Plasmid
    #155006
    Purpose
    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 E1418K/P1424Q/Y1426S variant (residues 1284-1462) from pcDNA3.1
    Depositor
    Insert
    PfEBA175
    Use
    Tags
    Flag, GFP, and signal peptide (residues 1 to 32) …
    Expression
    Mammalian
    Mutation
    E1418K/P1424Q/Y1426S; insert codes for EBA175 res…
    Promoter
    CMV
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
  4. pcDNA-GFP-EBA175-Flag(M1423R/P1424V/Y1426H)

    Plasmid
    #155312
    Purpose
    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 M1423R/P1424V/Y1426H variant (residues 1284-1462) from pcDNA3.1
    Depositor
    Insert
    PfEBA175
    Use
    Tags
    Flag, GFP, and signal peptide (residues 1 to 32) …
    Expression
    Mammalian
    Mutation
    M1423R/P1424V/Y1426H; insert codes for EBA175 res…
    Promoter
    CMV
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
  5. pcDNA-GFP-EBA175-Flag(A1419R/P1424Q/Y1426S)

    Plasmid
    #155007
    Purpose
    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 A1419R/P1424Q/Y1426S variant (residues 1284-1462) from pcDNA3.1
    Depositor
    Insert
    PfEBA175
    Use
    Tags
    Flag, GFP, and signal peptide (residues 1 to 32) …
    Expression
    Mammalian
    Mutation
    A1419R/P1424Q/Y1426S; insert codes for EBA175 res…
    Promoter
    CMV
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
  6. pcDNA-GFP-EBA175-Flag(S1421R/P1424Q/Y1426S)

    Plasmid
    #155008
    Purpose
    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 S1421R/P1424Q/Y1426S variant (residues 1284-1462) from pcDNA3.1
    Depositor
    Insert
    PfEBA175
    Use
    Tags
    Flag, GFP, and signal peptide (residues 1 to 32) …
    Expression
    Mammalian
    Mutation
    S1421R/P1424Q/Y1426S; insert codes for EBA175 res…
    Promoter
    CMV
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
  7. pcDNA-GFP-EBA175-Flag(P1424I/Y1425I/Y1426I)

    Plasmid
    #154977
    Purpose
    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424A/Y1425I/Y1426I variant (residues 1284-1462) from pcDNA3.1
    Depositor
    Insert
    PfEBA175
    Use
    Tags
    Flag, GFP, and signal peptide (residues 1 to 32) …
    Expression
    Mammalian
    Mutation
    P1424/Y1425I/Y1426I; insert codes for EBA175 resi…
    Promoter
    CMV
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
  8. pRS426-TUB1-internal6xHis - human TUBA1a Cterminal tail - delta Y

    Plasmid
    #60393
    Purpose
    Expression of chimeric yeast alpha tubulin (TUB1) - internal 6xHis with human alpha tubulin Cterminal tail (TUBA1a) without Cterminal tyrosine (delta Y)
    Depositor
    Insert
    TUB1 (TUB1 Budding Yeast, Synthetic)
    Use
    Tags
    Expression
    Yeast
    Mutation
    internal 6xHis (see supplement figure 4)
    Promoter
    GAL
    Available since
    Jan. 26, 2015
    Availability
    Academic Institutions and Nonprofits only
  9. pCMV6-AN-DDK_WT RAD23A_delta aa 161-201 (delta UBA1 domain)

    Plasmid
    #201463
    Purpose
    Expresses a mutant form of human RAD23A that lacks the UBA1 domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23A.
    Depositor
    Insert
    UV excision repair protein RAD23 homolog A (RAD23A Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Lacks residues 161-201
    Promoter
    Available since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
  10. pCMV6-AN-DDK_WT RAD23B_delta aa 188-228 (delta UBA1 domain)

    Plasmid
    #201546
    Purpose
    Expresses a mutant form of human RAD23B that lacks the UBA1 domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23B
    Depositor
    Insert
    UV excision repair protein RAD23 homolog B (RAD23B Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Lacks residues 188-228
    Promoter
    Available since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
  11. pcDNA-GFP-EBA175-Flag(M1423R/P1424V/Y1425W/Y1426H)

    Plasmid
    #155313
    Purpose
    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 M1423R/P1424V/Y1425W/Y1426H variant (residues 1284-1462) from pcDNA3.1
    Depositor
    Insert
    PfEBA175
    Use
    Tags
    Flag, GFP, and signal peptide (residues 1 to 32) …
    Expression
    Mammalian
    Mutation
    M1423R/P1424V/Y1425W/Y1426H; insert codes for EBA…
    Promoter
    CMV
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
  12. pcDNA-GFP-EBA175-Flag(E1418K/A1419R/P1424Q/Y1426S)

    Plasmid
    #155009
    Purpose
    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 E1418K/A1419R/P1424Q/Y1426S variant (residues 1284-1462) from pcDNA3.1
    Depositor
    Insert
    PfEBA175
    Use
    Tags
    Flag, GFP, and signal peptide (residues 1 to 32) …
    Expression
    Mammalian
    Mutation
    E1418K/A1419R/P1424Q/Y1426S; insert codes for EBA…
    Promoter
    CMV
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
  13. pcDNA-GFP-EBA175-Flag(E1418K/A1419R/S1421R/P1424Q/Y1426S)

    Plasmid
    #155010
    Purpose
    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 E1418K/A1419R/S1421R/P1424Q/Y1426S variant (residues 1284-1462) from pcDNA3.1
    Depositor
    Insert
    PfEBA175
    Use
    Tags
    Flag, GFP, and signal peptide (residues 1 to 32) …
    Expression
    Mammalian
    Mutation
    E1418K/A1419R/S1421/P1424Q/Y1426S; insert codes f…
    Promoter
    CMV
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
  14. pRS426-TUB1-internal6xHis - human TUBA1a Cterminal tail - C130S, E446C

    Plasmid
    #60394
    Purpose
    Expression of chimeric yeast alpha tubulin (TUB1) - internal 6xHis with human alpha tubulin Cterminal tail (TUBA1a) C130S, E446C for crosslinking polyglutamate peptide
    Depositor
    Insert
    TUB1 (TUB1 Budding Yeast, Synthetic)
    Use
    Tags
    Expression
    Yeast
    Mutation
    internal 6xHis (see supplement figure 4), amino a…
    Promoter
    GAL
    Available since
    Jan. 26, 2015
    Availability
    Academic Institutions and Nonprofits only
  15. pGEX4T-1-6AlanineHE1

    Plasmid
    #165098
    Purpose
    bacterial expression of a GST fusion of Human Uba1 with a 6alanine linker between the GST encoded by the plasmid and the inserted Uba1
    Depositor
    Insert
    Uba1 (UBA1 Human)
    Use
    Tags
    GST-6-alanine
    Expression
    Bacterial
    Mutation
    None
    Promoter
    Available since
    March 10, 2021
    Availability
    Academic Institutions and Nonprofits only
Showing: 121 - 140 of 537 results