We narrowed to 203 results for: dysf

1. pLPC-N-Myc-Flag-BirA-hPOT1-DeltaOB

   Plasmid

   #166410

   Purpose

   express BirA-hPOT1 ΔOB in mammalian cells

   Depositor

   Agnel Sfeir

   Article

   Pinzaru et al Genes Dev. 2020 Dec 1;34(23-24):1619-163

   Insert

   POT1 ΔOB (POT1 Human)
   

   Use

   Tags

   MYC-flag-BirA

   Expression

   Mammalian

   Mutation

   ΔOB

   Promoter

   cmv

   Available since

   April 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLVX-TetOne-Puro-UCP1-Flag

   Plasmid

   #234983

   Purpose

   Expresses UCP1 with C-terminal Flag tag in human cell lines

   Depositor

   Denis Titov

   Article

   Choe et al Cell Chem Biol. 2025 Apr 17;32(4):620-63

   Insert

   UCP1 (Ucp1 Mouse)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-Ef1a-DIO hChR2(C128S/D156A)-EYFP

   Plasmid

   #35503

   Purpose

   AAV expression of Ef1a-driven, cre-dependent, stabilized step function opsin (SSFO) for optogenetic activation.

   Depositor

   Karl Deisseroth

   Article

   Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

   Insert

   hChR2(C128S/D156A)-EYFP
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   C128S and D156A

   Promoter

   Ef1a

   Available since

   April 23, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-Ef1a-DIO C1V1 (t/t)-TS-mCherry

   Plasmid

   #35498

   Purpose

   AAV expression of Ef1a-driven, cre-dependent, chimeric opsin variant C1V1 (E122T/E162T) for fast and potent optical excitation at red-shifted wavelengths.

   Depositor

   Karl Deisseroth

   Article

   Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

   Insert

   ChR1-VChR1 Chimera
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   E122T and E162T

   Promoter

   Ef1a

   Available since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHAGE-EF1aL-WT_ABCA3-GFP

   Plasmid

   #188548

   Purpose

   Lentiviral vector allowing for EF1aL-driven constitutive expression of wildtype human ABCA3:GFP fusion protein

   Depositor

   Darrell Kotton

   Article

   Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

   Insert

   ABCA3:GFP (ABCA3 Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mutation

   Promoter

   EF1aL

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CaMKIIa-C1V1 (t/t)-TS-mCherry

   Plasmid

   #35500

   Purpose

   AAV expression of CaMKII-driven chimeric opsin variant C1V1 (E122T/E162T) for fast and potent optical excitation at red-shifted wavelengths.

   Depositor

   Karl Deisseroth

   Article

   Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

   Insert

   ChR1-VChR1 Chimera
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   E122T and E162T

   Promoter

   CaMKIIa

   Available since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CaMKIIa-C1V1 (t/t)-TS-EYFP

   Plasmid

   #35499

   Purpose

   AAV expression of CaMKII-driven chimeric opsin variant C1V1 (E122T/E162T) for fast and potent optical excitation at red-shifted wavelengths.

   Depositor

   Karl Deisseroth

   Article

   Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

   Has Service

   AAV9

   Insert

   ChR1-VChR1 Chimera
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   E122T and E162T

   Promoter

   CaMKIIa

   Available since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV-Ef1a-DIO C1V1 (t/t)-TS-EYFP

   Plasmid

   #35497

   Purpose

   AAV expression of Ef1a-driven, cre-dependent, chimeric opsin variant C1V1 (E122T/E162T) for fast and potent optical excitation at red-shifted wavelengths.

   Depositor

   Karl Deisseroth

   Article

   Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

   Has Service

   AAV9

   Insert

   ChR1-VChR1 Chimera
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   E122T and E162T

   Promoter

   Ef1a

   Available since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-Ef1a-DIO hChR2(C128S/D156A)-mCherry

   Plasmid

   #35504

   Purpose

   AAV expression of Ef1a-driven, cre-dependent, stabilized step function opsin (SSFO) for optogenetic activation.

   Depositor

   Karl Deisseroth

   Article

   Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

   Insert

   hChR2(C128S/D156A)-mCherry
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   C128S and D156A

   Promoter

   Ef1a

   Available since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-CaMKIIa-hChR2(C128S/D156A)-mCherry

    Plasmid

    #35502

    Purpose

    AAV expression of CaMKII-driven stabilized step function opsin (SSFO) for optogenetic activation.

    Depositor

    Karl Deisseroth

    Article

    Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

    Insert

    hChR2(C128S/D156A)-mCherry
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    C128S and D156A

    Promoter

    CaMKIIa

    Available since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMXs-WT-ATPIF1

    Plasmid

    #85403

    Purpose

    Retroviral vector expressing wild type human ATPIF1

    Depositor

    David Sabatini

    Article

    Chen et al Cell Rep. 2014 Apr 10;7(1):27-34. doi: 1

    Insert

    ATPIF1 (ATP5IF1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Wildtype

    Promoter

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

12. hTln1-R12DD-SCLS

    Plasmid

    #191443

    Purpose

    expresses a variant of human talin1 domains R12-DD, with a deletion between K2375 and A2397

    Depositor

    Ben Goult

    Article

    Elefant et al JCI Insight. 2024 Dec 20;9(24):e173664.

    Insert

    Human Talin1 R12DD (TLN1 Human)
    

    Use

    Tags

    His-tag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    deletion between residues K2375 and A2397

    Promoter

    Available since

    Dec. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRK5-EGFP-Tau P301L

    Plasmid

    #46908

    Purpose

    expresses EGFP tagged Tau P301L in mammalian cells

    Depositor

    Karen Ashe

    Article

    Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

    Insert

    Tau P301L (MAPT Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    P301L

    Promoter

    CMV

    Available since

    July 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCK005_U6-Sp-sgRNA_UP-TdTomato_WPRE

    Plasmid

    #85453

    Purpose

    Lentiviral CRISPR system backbone bearing BsmBI site for S. pyogenes new guide RNAs and TdTomato.

    Depositor

    Aviv Regev

    Article

    Singer et al Cell. 2016 Sep 8;166(6):1500-1511.e9. do

    Insert

    U6-sgRNA-Backbone-UP-TdTomato
    

    Use

    CRISPR and Lentiviral

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    deletion of AA123-365 in tdTomato (please see dep…

    Promoter

    Available since

    April 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCW57.kc1-HSD17B10

    Plasmid

    #164076

    Purpose

    Human codon optimized HSD17B10. Expression is controlled by doxycycline.

    Depositor

    David Sabatini

    Article

    Condon et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Insert

    HSD17B10 (HSD17B10 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHAGE-EF1aL-E690Kmut_ABCA3-GFP

    Plasmid

    #188549

    Purpose

    Lentiviral vector allowing for EF1aL-driven constitutive expression of E690K mutant human ABCA3:GFP fusion protein

    Depositor

    Darrell Kotton

    Article

    Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

    Insert

    ABCA3:GFP (ABCA3 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mutation

    amino acid E690->K690 (ABCA3 gene)

    Promoter

    EF1aL

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHAGE-EF1aL-W308Rmut_ABCA3-GFP

    Plasmid

    #188550

    Purpose

    Lentiviral vector allowing for EF1aL-driven constitutive expression of W308R mutant human ABCA3:GFP fusion protein

    Depositor

    Darrell Kotton

    Article

    Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

    Insert

    ABCA3 (ABCA3 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mutation

    amino acid W308->R308 (ABCA3 gene)

    Promoter

    EF1aL

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRK5-EGFP-Tau

    Plasmid

    #46904

    Purpose

    expresses EGFP tagged Tau in mammalian cells

    Depositor

    Karen Ashe

    Article

    Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

    Insert

    MAPT (MAPT Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    This WT htau construct contains human four-repeat…

    Promoter

    CMV

    Available since

    July 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCK002_U6-Sa-sgRNA(mod)_EFS-SaCas9-2A-Puro_WPRE

    Plasmid

    #85452

    Purpose

    Lentiviral vector encoding modified SaCas9 system backbone bearing BsmBI site for new guide RNAs and puromycin selection marker.

    Depositor

    Aviv Regev

    Article

    Singer et al Cell. 2016 Sep 8;166(6):1500-1511.e9. do

    Insert

    U6-modified-sgRNA-Backbone-EFS-hSaCas9-2xNLS-2A-Puro
    

    Use

    CRISPR and Lentiviral

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV hPGRN

    Plasmid

    #213682

    Purpose

    Expresses human progranulin (PGRN) with an N-terminal twin-Strep-V5 tag

    Depositor

    Thomas Kukar

    Article

    Root et al Cell Rep. 2024 Nov 19;43(12):114985. doi

    Insert

    Human Progranulin (hPGRN) (GRN Human)
    

    Use

    AAV

    Tags

    Twin-Strep tag and V5 tag (after signal peptide)

    Expression

    Mutation

    Promoter

    cytomegalovirus enhancer/chicken β-actin promoter

    Available since

    Dec. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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