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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 11,159 results for: ena

Showing: 121 - 140 of 11159 results

  1. pRDA_936

    Plasmid
    #216094
    Purpose
    Cas12a [EnAs] knockout targeting CD46, CD47, CD55, CD81, positive control
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Insert
    CD46, CD47, CD55, CD81 guides
    Use
    CRISPR and Lentiviral; Positive controls
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    216094-plasmid-map-sequence-id-431378

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  4. AA054

    Plasmid
    #215932
    Purpose
    Fragmid fragment: (guide cassette) guide expression; reverse orientation
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    rev{U6_v1; DR_v0 [EnAs]; BsmBI_v2; BsmBI_v3}
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    215932-plasmid-map-sequence-id-431474

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  5. AA016

    Plasmid
    #215927
    Purpose
    Fragmid fragment: (guide cassette) guide expression
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    U6_v1; DR_v0 [EnAs]; BsmBI_v2; BsmBI_v3
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    215927-plasmid-map-sequence-id-431438

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  6. AA102

    Plasmid
    #215935
    Purpose
    Fragmid fragment: (guide cassette) guide expression; reverse orientation; enables iBar UMIs
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    rev{U6_v1; DR_v0 [EnAs]; BsmBI_v2; BsmBI_v5}
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    215935-plasmid-map-sequence-id-431435

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  7. AA107

    Plasmid
    #215936
    Purpose
    Fragmid fragment: (guide cassette) guide expression; enables iBar UMIs
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    U6_v1; DR_v0 [EnAs]; BsmBI_v2; BsmBI_v5
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    215936-plasmid-map-sequence-id-431416

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  9. pRDB_131

    Plasmid
    #216095
    Purpose
    Cas12a [EnAs] CRISPRa targeting CD97, CD4, CD26, CD274, positive control
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Insert
    CD97, CD4, CD26, CD274 guides
    Use
    CRISPR and Lentiviral; Positive controls
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    216095-plasmid-map-sequence-id-431381

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  11. pRL_GI_PCB28

    Plasmid
    #98745
    Purpose
    For phycocyanobilin production in Saccharomyces cerevisiae. Contains integrative cassette for the yeast his1-Δ200 locus. Encodes constitutively expressed HY1 and PcyA.
    Depositor
    Bernd Müller-Röber
    Article
    Hochrein et al Nucleic Acids Res. 2017 Sep 6;45(15):919
    Inserts
    heme oxygenase 1 (TED4 Mustard Weed)
    Phycocyanobilin:ferredoxin oxidoreductase
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Codon optimized for Saccharomyces cerevisiae
    Promoter
    ADH1m and PGK1
    Available since
    Aug. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98745-plasmid-map-sequence-id-189854

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  15. pET24a MDHM1_ARATH

    Plasmid
    #204236
    Purpose
    Bacterial expression of Arabidopsis mitochondrial malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    MDHM1_ARATH (mMDH1 Mustard Weed)
    Use
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    Does done include the first 52 residues that are …
    Promoter
    Lac
    Available since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204236-plasmid-map-sequence-id-413347

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  16. pET24a MDHNP_ARATH

    Plasmid
    #204238
    Purpose
    Bacterial expression of Arabidopsis chloroplastic malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    MDHNP_ARATH (AT5G58330 Mustard Weed)
    Use
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    Does not include the chloroplastic transit sequen…
    Promoter
    Lac
    Available since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204238-plasmid-map-sequence-id-413341

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  17. pET28a hMDH2

    Plasmid
    #204230
    Purpose
    Bacterial expression of mitochondrial human malate dehydrogenase 2 (hMDH2) transcript variant 1; Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    hMDH2 (MDH2 Human)
    Use
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    Does not include transit peptide and includes a M…
    Promoter
    Lac
    Available since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204230-plasmid-map-sequence-id-413333

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  18. pVP91A-pcaHG

    Plasmid
    #113766
    Purpose
    Expresses the alpha (pcaG) and the beta (pcaH) subunits of Protocatechuate 3,4-Dioxygenase from Pseudomonas putida.
    Depositor
    John Lipscomb
    Article
    Knoot et al Proc Natl Acad Sci U S A. 2015 Jan 13;11
    Inserts
    β subunit of Protocatechuate 3,4- Dioxygenase
    α subunit of Protocatechuate 3,4- Dioxygenase
    Use
    Tags
    Met-6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    T5 promoter
    Available since
    Aug. 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    113766-plasmid-map-sequence-id-218346

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Showing: 121 - 140 of 11159 results