We narrowed to 1,121 results for: gibson assembly
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Plasmid#216312PurposeExpresses nanoHER2-SpyTag003 in bacterial cells. nanoHER2 is an anti-HER2 nanobody.DepositorInsertnanoHER2-SpyTag003
TagsHis6 tag and SpyTag003ExpressionBacterialPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGK:HygroR-CMV:mKate2-B_UTR
Plasmid#68480PurposeLentivirus expressing mKate2 with UTR sensor cassette and hygromycin resistanceDepositorInsertsHygromycin Resistance
mKate2-B_UTR
UseLentiviralExpressionMammalianMutationshRNA sensor cassette "B" added to 3…PromoterCMV and pGKAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
MSCV Puro-pGK:GFP
Plasmid#68486PurposeRetrovirus expressing GFP driven by pGK promoterDepositorInsertsPuromycin Resistance
GFP
UseRetroviralExpressionMammalianPromoterLTR and pGKAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLX-AS
Plasmid#188870PurposeCompact T-DNA binary vector of the pLX series suitable for assembly of plant virus infectious clones and their Agrobacterium-mediated delivery to plantsDepositorInsertnone
UseSynthetic Biology; Binary t-dna vectorExpressionPlantPromotercauliflower mosaic virus (CaMV) 35S promoterAvailable sinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPW3758 : CMVd3-HsIRE1-HaloTag
Plasmid#185680PurposeLow-level transient expression of full-length HsIRE1 with a C-terminal HaloTag.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertERN1 (ERN1 Human, Synthetic)
ExpressionMammalianAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTRE:shRNA A-pGK:rtTA-Cre
Plasmid#68471PurposeInducible lentivirus expressing an arbitrary shRNA and CreDepositorInsertsGFP-shRNA A-miR30
rtTA-2A-Cre
UseLentiviral and RNAiExpressionMammalianPromoterpGK and pTREAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
MSCV Puro-CCSP:GFP
Plasmid#68487PurposeRetrovirus expressing GFP driven by CCSP promoterDepositorInsertsPuromycin Resistance
GFP
UseRetroviralExpressionMammalianPromoterCCSP and LTRAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
MSCV Puro-UBC:GFP
Plasmid#68482PurposeRetrovirus expressing GFP driven by UBC promoterDepositorInsertsPuromycin Resistance
GFP
UseRetroviralExpressionMammalianPromoterLTR and UBCAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBIG2abc
Plasmid#80617PurposepBIG2abc - Step2 vector abc (biGBac system for expression of protein complexes in insect cells)DepositorTypeEmpty backboneExpressionInsectAvailable sinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBTK402
Plasmid#110598PurposeBTK Type 8 Bacterial origin (broad-host-range) for golden gate assemblyDepositorInsertmRFP dropout (RSF1010 origin)
UseSynthetic BiologyAvailable sinceFeb. 14, 2020AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pBIG2abcde
Plasmid#80619PurposepBIG2abcde - Step2 vector abcde (biGBac system for expression of protein complexes in insect cells)DepositorTypeEmpty backboneExpressionInsectAvailable sinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
STAGR_gRNAScaffold_mU6
Plasmid#102844PurposeCan be used as PCR template for a STAgR reactionDepositorInsertSTAgR Insert gRNAScaffold_mU6
UsePcr template for stagr insertsPromotermU6Available sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
TOPO tTR-KRAB-rtTA3-Luc
Plasmid#68449PurposeTOPO construct expressing tTR-KRAB-rtTA3-Luc for generation of GMAP-compatible geneDepositorInserttTR-KRAB-2A-rtTA3-2A-Luciferase
UseLuciferase; GmapExpressionBacterialPromoterPlacAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
B4-4
Plasmid#111478Purposetranslational release factor 1DepositorInserttranslational release factor 1
Tags6xHisExpressionBacterialAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBIG1c
Plasmid#80613PurposepBIG1c - Step1 vector c (biGBac system for expression of protein complexes in insect cells)DepositorTypeEmpty backboneExpressionInsectAvailable sinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBIG2abcd
Plasmid#80618PurposepBIG2abcd - Step2 vector abcd (biGBac system for expression of protein complexes in insect cells)DepositorTypeEmpty backboneExpressionInsectAvailable sinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMAP L1A
Plasmid#153343PurposeMobius Assembly cloning and expression vector for plants with high-copy number origin of replicationDepositorPublicationTypeEmpty backboneExpressionPlantAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNA0304
Plasmid#165612PurposeVector for expression of sgRNAs in yeast: SNR52p-NotI-sgRNA-SUP4t (NotI site in place of the spacer)DepositorInsertSNR52p-NotI-sgRNA-SUP4t (S. cerevisiae SNR52 promoter driving the sgRNA for SpCas9, with a NotI site in place of the spacer)
UseCRISPRExpressionYeastMutationNotI site replaced the CAN1 spacer in parent vect…PromoterSNR52Available sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBIG1d
Plasmid#80614PurposepBIG1d - Step1 vector d (biGBac system for expression of protein complexes in insect cells)DepositorTypeEmpty backboneExpressionInsectAvailable sinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBIG1e
Plasmid#80615PurposepBIG1e - Step1 vector e (biGBac system for expression of protein complexes in insect cells)DepositorTypeEmpty backboneExpressionInsectAvailable sinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by