We narrowed to 1,124 results for: gibson assembly

Showing: 121 - 140 of 1124 results

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pQE80L SpyTag-ELP-SpyTag

Plasmid

#112634

Purpose

Two SpyTags linked by an elastin-like polypeptide for hydrogel formation

Depositor

Mark Howarth

Article

Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:

Insert

SpyTag-ELP-SpyTag

Tags

His6

Expression

Bacterial

Mutation

central cysteine

Promoter

T5

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-IMX-DogTag

Plasmid

#113765

Purpose

Expresses IMX-DogTag in bacterial cells for modular antigen multimerization using SnoopLigase

Depositor

Mark Howarth

Article

Andersson et al Sci Rep. 2019 Mar 15;9(1):4625. doi: 10.

Insert

IMX-DogTag

Expression

Bacterial

Promoter

T7

Available Since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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pDD379

Plasmid

#91834

Purpose

SapTrap destination vector for building combined sgRNA expression + repair template vectors, using the F+E sgRNA scaffold

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Type

Empty backbone

Use

CRISPR

Expression

Worm

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pDD121

Plasmid

#91833

Purpose

Cas9 expression in C. elegans

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

Cas9

Use

CRISPR

Expression

Worm

Mutation

Codon optimized and with synthetic introns for C.…

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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EGFP-VAV3

Plasmid

#242834

Purpose

Encodes mouse VAV3 and N-terminal EGFP tag

Depositor

Cheng-han Yu

Article

Qi et al Cell Mol Life Sci. 2025 Mar 24;82(1):127

Insert

VAV3 (Vav3 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pMulti_K_ccdB

Plasmid

#223852

Purpose

GEC-acceptor for 4G-cloning, designed for expression in E. coli (Kanamyicin resistance)

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Type

Empty backbone

Use

Golden-gate acceptor

Expression

Bacterial

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pMulti_A_ccdB

Plasmid

#223851

Purpose

GEC-acceptor for 4G-cloning, designed for expression in E. coli (Ampicillin resistance)

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Type

Empty backbone

Use

Golden-gate acceptor

Expression

Bacterial

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Ct)_MYC9

Plasmid

#223897

Purpose

4G-cloning donor for C-terminal MYC9 tag

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

Myc9

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Ct)_PK6

Plasmid

#223898

Purpose

4G-cloning donor for C-terminal PK6 tag

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

PK6

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Ct)_FLAG

Plasmid

#223895

Purpose

4G-cloning donor for C-terminal FLAG tag

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

FLAG

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Ct)_HALO

Plasmid

#223899

Purpose

4G-cloning donor for C-terminal HALO tag

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

HALO

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Nt)_HALO

Plasmid

#223885

Purpose

4G-cloning donor for N-terminal HALO tag

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

HALO

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Nt)_GFP

Plasmid

#223880

Purpose

4G-cloning donor for N-terminal GFP tag

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

GFP

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Ct)_His8

Plasmid

#223886

Purpose

4G-cloning donor for C-terminal His8 tag

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

His8

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Ct)_TS

Plasmid

#223891

Purpose

4G-cloning donor for C-terminal TwinStrep tag

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

TwinStrep

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pFD-NIa-Protease-TVMV

Plasmid

#137109

Purpose

iFLinkC Linker cloning

Depositor

Viktor Stein

Article

Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

Insert

NIa Protease TVMV

Use

Synthetic Biology

Available Since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pBTK519

Plasmid

#110603

Purpose

BTK assembled plasmid- broad-host-range GFP expression from PA1 promoter (kanamycin resistance)

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

GFP

Use

Synthetic Biology

Available Since

Feb. 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pBTK501

Plasmid

#110602

Purpose

BTK assembled plasmid- broad-host-range GFP expression from PA1 promoter (ampicillin resistance)

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

GFP

Use

Synthetic Biology

Available Since

Feb. 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pBTK402

Plasmid

#110598

Purpose

BTK Type 8 Bacterial origin (broad-host-range) for golden gate assembly

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

mRFP dropout (RSF1010 origin)

Use

Synthetic Biology

Available Since

Feb. 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pBTK520

Plasmid

#110604

Purpose

BTK assembled plasmid- broad-host-range GFP expression from PA1 promoter (spectinomycin resistance)

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

GFP

Use

Synthetic Biology

Available Since

Feb. 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge