We narrowed to 11,303 results for: wus;

1. pcDNA3.1 LIC 6A FLAG-TEV-DPP9S KK93EE

   Plasmid

   #166796

   Purpose

   Mammalian cell expression vector for FLAG-tagged DPP9S. KK93EE mutant is partial loss of CARD8 binding.

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   DPP9 (DPP9 Human)
   

   Use

   Tags

   FLAG-TEV

   Expression

   Mammalian

   Mutation

   KK93EE

   Promoter

   CMV

   Available Since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3.1 LIC 6A FLAG-TEV-DPP9S E597R

   Plasmid

   #166800

   Purpose

   Mammalian cell expression vector for FLAG-tagged DPP9S. E597R mutant is partial loss of CARD8 binding.

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   DPP9 (DPP9 Human)
   

   Use

   Tags

   FLAG-TEV

   Expression

   Mammalian

   Mutation

   E597R

   Promoter

   CMV

   Available Since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDB.His.MBP.3C NLRP1 UPA WT

   Plasmid

   #164010

   Purpose

   Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged NLRP1 UPA

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Tags

   His6-MBP-3C

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. TRBV16

   Plasmid

   #123451

   Purpose

   construction of TCR

   Depositor

   Catherine Wu

   Article

   Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

   Insert

   TRBV16 (TRBV16 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   Available Since

   May 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGL3Basic-Mdm3-T5

   Plasmid

   #32373

   Depositor

   Hong Wu

   Article

   Chang et al J Biol Chem. 2004 Jul 9;279(28):29841-8.

   Insert

   Mdm2 promoter (Mdm2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

6. pFastBac HTB MBP-SARM1 A600G

   Plasmid

   #195764

   Purpose

   Purification of SARM1 from insect cells

   Depositor

   Hao Wu

   Insert

   SARM1 (SARM1 Human)
   

   Use

   Tags

   HIS-MBP-TEV

   Expression

   Insect

   Mutation

   A600G

   Promoter

   polyhedrin

   Available Since

   Feb. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFastBac HTB MBP-SARM1

   Plasmid

   #195763

   Purpose

   Purification of SARM1 from insect cells

   Depositor

   Hao Wu

   Insert

   SARM1 (SARM1 Human)
   

   Use

   Tags

   HIS-MBP-TEV

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin

   Available Since

   Feb. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLV-SARS-CoV-2 5’UTR ΔSL1-Luciferase

   Plasmid

   #191481

   Purpose

   Luciferase reporter for SARS-CoV-2 5' UTR ΔSL1

   Depositor

   Hao Wu

   Article

   Shi et al bioRxiv. 2020 Sep 18. doi: 10.1101/2020.

   Insert

   SARS-CoV-2 5' UTR ΔSL1
   

   Use

   Lentiviral and Luciferase

   Tags

   Expression

   Mutation

   Missing the stem-loop 1 region of SARS-CoV-2 5&#x…

   Promoter

   Available Since

   Nov. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV-Dest-MBP-Nsp1

   Plasmid

   #191483

   Purpose

   Mammalian expression of MBP-tagged SARS-CoV-2 Nsp1

   Depositor

   Hao Wu

   Article

   Vora et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

   Insert

   MBP-Nsp1 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1 LIC 6A CARD8-FLAG F405G

    Plasmid

    #169956

    Purpose

    Mammalian expression of CARD8-FLAG with a site III mutation (F405G)

    Depositor

    Hao Wu

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    F405G

    Promoter

    CMV

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1 LIC 6A CARD8 Ub-S297-FLAG F370G

    Plasmid

    #169960

    Purpose

    Mammalian expression of the CARD8 C-terminus with an interface III mutation fused to ubiquitin (co-translationally processed)

    Depositor

    Hao Wu

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    FLAG and Ub

    Expression

    Mammalian

    Mutation

    F370G

    Promoter

    CMV

    Available Since

    July 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1 LIC 6A CARD8-FLAG R394E

    Plasmid

    #169954

    Purpose

    Mammalian expression of CARD8-FLAG with a site III mutation (R394E)

    Depositor

    Hao Wu

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    R394E

    Promoter

    CMV

    Available Since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1 LIC 6A CARD8-FLAG R394E + S297A

    Plasmid

    #169955

    Purpose

    Mammalian expression of CARD8-FLAG with a site III mutation (R394E) with an additional autoprocessing mutation (S297A)

    Depositor

    Hao Wu

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S297A, R394E

    Promoter

    CMV

    Available Since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1 LIC 6A CARD8-FLAG F405G + S297A

    Plasmid

    #169957

    Purpose

    Mammalian expression of CARD8-FLAG with a site III mutation (F405G) with an additional autoprocessing mutation (S297A)

    Depositor

    Hao Wu

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S297A, F405G

    Promoter

    CMV

    Available Since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1 LIC 6A CARD8 Ub-S297-FLAG L368G

    Plasmid

    #169959

    Purpose

    Mammalian expression of the CARD8 C-terminus with an interface III mutation fused to ubiquitin (co-translationally processed)

    Depositor

    Hao Wu

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    FLAG and Ub

    Expression

    Mammalian

    Mutation

    L368G

    Promoter

    CMV

    Available Since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1 LIC 6A CARD8 Ub-S297-FLAG R394E

    Plasmid

    #169961

    Purpose

    Mammalian expression of the CARD8 C-terminus with an interface III mutation fused to ubiquitin (co-translationally processed)

    Depositor

    Hao Wu

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    FLAG and Ub

    Expression

    Mammalian

    Mutation

    R394E

    Promoter

    CMV

    Available Since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1 LIC 6A CARD8 Ub-S297-FLAG F405G

    Plasmid

    #170119

    Purpose

    Mammalian expression of the CARD8 C-terminus with an interface III mutation fused to ubiquitin (co-translationally processed).

    Depositor

    Hao Wu

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    FLAG and Ub

    Expression

    Mammalian

    Mutation

    F405G

    Promoter

    CMV

    Available Since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1 LIC 6A CARD8 Ub-S297-FLAG

    Plasmid

    #169958

    Purpose

    Mammalian expression of the CARD8 C-terminus fused to ubiquitin (co-translationally processed)

    Depositor

    Hao Wu

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    FLAG and Ub

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1 LIC 6A CARD8-FLAG L368G

    Plasmid

    #169952

    Purpose

    Mammalian expression of CARD8-FLAG with a site III mutation (L368G)

    Depositor

    Hao Wu

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    L368G

    Promoter

    CMV

    Available Since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1 LIC 6A CARD8-FLAG E274R

    Plasmid

    #169950

    Purpose

    Mammalian expression of CARD8-FLAG that cannot bind DPP9 due to a mutation at site I (E274R)

    Depositor

    Hao Wu

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    E274R

    Promoter

    CMV

    Available Since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge