We narrowed to 5,540 results for: KRAS

1. pLX304-BCL-XL-F97W

   Plasmid

   #203572

   Purpose

   Expresses V5-tagged BCL-XL with partial drug resistance to A-1331852 in mammalian cells.

   Depositor

   Abhishek Chakraborty

   Article

   Grubb et al Clin Cancer Res. 2022 Nov 1;28(21):4689-

   Insert

   BCL2L1 (BCL2L1 Human)
   

   Use

   Lentiviral

   Tags

   V5-tagged

   Expression

   Mammalian

   Mutation

   Changed Phenylalanine 97 to Tryptophan for partia…

   Promoter

   CMV

   Available Since

   Dec. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. PX458-3xHA-dFnCas9

   Plasmid

   #201954

   Purpose

   Mammalian expression plasmid of dead FnCas9 with T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Debojyoti Chakraborty

   Article

   Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

   Insert

   3xHA-NLS-dFnCas9-T2A-EGFP
   

   Use

   CRISPR

   Tags

   3xHA, NLS, and T2A-EGFP

   Expression

   Mammalian

   Mutation

   D11A and H969A on FnCas9

   Promoter

   Cbh

   Available Since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-His6-en1dFnCas9GFP

   Plasmid

   #201950

   Purpose

   Expression of en1dFnCas9-GFP in bacterial cells

   Depositor

   Debojyoti Chakraborty

   Article

   Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

   Insert

   Expression of en1dFnCas9-GFP in bacterial cells
   

   Use

   CRISPR

   Tags

   6x His, HA, NLS, and mEGFP

   Expression

   Bacterial

   Mutation

   E1369R on FnCas9

   Promoter

   T7

   Available Since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-His6-en15dFnCas9GFP

   Plasmid

   #201951

   Purpose

   Expression of en15dFnCas9-GFP in bacterial cells

   Depositor

   Debojyoti Chakraborty

   Article

   Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

   Insert

   Expression of en15dFnCas9-GFP in bacterial cells
   

   Use

   CRISPR

   Tags

   6x His, HA, NLS, and mEGFP

   Expression

   Bacterial

   Mutation

   E1603H on FnCas9

   Promoter

   T7

   Available Since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. PX458-3xHA-dSpCas9

   Plasmid

   #201953

   Purpose

   Mammalian expression plasmid of dead SpCas9 with T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Debojyoti Chakraborty

   Article

   Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

   Insert

   3xHA-NLS-dSpCas9-T2A-EGFP
   

   Use

   CRISPR

   Tags

   3xHA, NLS, and T2A-EGFP

   Expression

   Mammalian

   Mutation

   D10A and H840A on SpCas9

   Promoter

   Cbh

   Available Since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLX304-BCL-XL-499R

   Plasmid

   #203574

   Purpose

   Expresses V5-tagged BCL-XL with resistance to shRNA (TRCN0000033499) in mammalian cells.

   Depositor

   Abhishek Chakraborty

   Article

   Grubb et al Clin Cancer Res. 2022 Nov 1;28(21):4689-

   Insert

   BCL2L1 (BCL2L1 Human)
   

   Use

   Lentiviral

   Tags

   V5- tagged

   Expression

   Mammalian

   Mutation

   silent point mutations introduced to make it resi…

   Promoter

   CMV

   Available Since

   Feb. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP-N1-PARP1

   Plasmid

   #211578

   Purpose

   CMV driven PARP1-eGFP expressing construct with IRES-Neomycin selection

   Depositor

   Chris Lord

   Article

   Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

   Insert

   PARP1 (PARP1 Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-N1-PARP1-E988K

   Plasmid

   #211580

   Purpose

   CMV driven PARP1-E988K -eGFP expressing construct with IRES-Neomycin selection

   Depositor

   Chris Lord

   Article

   Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

   Insert

   PARP1 (PARP1 Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Contains E988K mutation

   Available Since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. PB-PARP1-del.p119K120S–eGFP

   Plasmid

   #211554

   Purpose

   A piggyBac vector containing CMV-PARP1-del.p119K120S-eGFP-IRES-Neo cassette.

   Depositor

   Chris Lord

   Article

   Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

   Insert

   PARP1 (PARP1 Human)
   

   Use

   Piggybac

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   deletion of amino acids 119K 120S

   Promoter

   CMV

   Available Since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFP-N1-PARP1-delM43;F44I

    Plasmid

    #211579

    Purpose

    CMV driven PARP1-delM43;F44I -eGFP expressing construct with IRES-Neomycin selection

    Depositor

    Chris Lord

    Article

    Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

    Insert

    PARP1 (PARP1 Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Contains deletion of M43 and F44I

    Available Since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. PB-PARP1–eGFP

    Plasmid

    #211552

    Purpose

    A piggyBac vector containing CMV-PARP1-eGFP-IRES-Neo cassette.

    Depositor

    Chris Lord

    Article

    Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

    Insert

    PARP1 (PARP1 Human)
    

    Use

    Piggybac

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. PX458-3xHA-en31FnCas9

    Plasmid

    #201949

    Purpose

    Mammalian expression plasmid of enhanced activity FnCas9 variant en31FnCas9 with T2A-EGFP and cloning backbone for sgRNA

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

    Insert

    3xHA-NLS-en31FnCas9-T2A-EGFP
    

    Use

    CRISPR

    Tags

    3xHA, NLS, and T2A-EGFP

    Expression

    Mammalian

    Mutation

    G1243T, E1369R, E1603H on FnCas9

    Promoter

    Cbh

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. PX458-3xHA-SpCas9-HF1

    Plasmid

    #201952

    Purpose

    Mammalian expression plasmid of high-fidelity SpCas9 variant SpCas9-HF1 with T2A-EGFP and cloning backbone for sgRNA

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

    Insert

    Mammalian expression plasmid of high-fidelity SpCas9 variant SpCas9-HF1 with T2A-EGFP and cloning backbone for sgRNA
    

    Use

    CRISPR

    Tags

    3xHA, NLS, and T2A-EGFP

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PB-PARP1-Apex2–eGFP

    Plasmid

    #211555

    Purpose

    A piggyBac vector containing CMV-PARP1-Apex2-eGFP-IRES-Neo cassette.

    Depositor

    Chris Lord

    Article

    Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

    Insert

    PARP1 (PARP1 Human)
    

    Use

    Piggybac

    Tags

    Apex2-eGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. PX458-3xHA-en1FnCas9

    Plasmid

    #201947

    Purpose

    Mammalian expression plasmid of enhanced activity FnCas9 variant en1FnCas9 with T2A-EGFP and cloning backbone for sgRNA

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

    Insert

    3xHA-NLS-en1FnCas9-T2A-EGFP
    

    Use

    CRISPR

    Tags

    3xHA, NLS, and T2A-EGFP

    Expression

    Mammalian

    Mutation

    E1369R on FnCas9

    Promoter

    Cbh

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. R713-M91-303: CMV51p> Halotag-Hs.NRAS

    Plasmid

    #159682

    Purpose

    Mammalian protein expression of NRAS-Halotag fusion

    Depositor

    Dominic Esposito

    Article

    Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

    Insert

    Halotag-Hs.NRAS (NRAS Human)
    

    Tags

    Halotag

    Expression

    Mammalian

    Available Since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET-His6-FnCas9GFP

    Plasmid

    #130966

    Purpose

    expression of FnCas9-GFP in bacterial cells

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Proc Natl Acad Sci U S A. 2019 Oct 15;11

    Insert

    HA-NLS-FnCas9
    

    Use

    CRISPR

    Tags

    6xHis-mEGFP-TEV-HA-NLS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PX458-3xHA-en15FnCas9

    Plasmid

    #201948

    Purpose

    Mammalian expression plasmid of enhanced activity FnCas9 variant en15FnCas9 with T2A-EGFP and cloning backbone for sgRNA

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

    Insert

    3xHA-NLS-en15FnCas9-T2A-EGFP
    

    Use

    CRISPR

    Tags

    3xHA, NLS, and T2A-EGFP

    Expression

    Mammalian

    Mutation

    E1603H on FnCas9

    Promoter

    Cbh

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGEX4T1-TEV-HsFbxo7-129-398/Skp1

    Plasmid

    #202592

    Purpose

    Bacterial expression of HsFbxo7-129-398 and HsSkp1

    Depositor

    Brenda Schulman

    Article

    Kraus et al EMBO Rep. 2023 Aug 3;24(8):e56399. doi:

    Inserts

    Fbxo7 (FBXO7 Human)
    Skp1 (SKP1 Human)
    

    Tags

    GST, TEV

    Expression

    Bacterial

    Mutation

    aa 129-398 only

    Available Since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. R713-M52-303: CMV51p> Halotag-NRasHVR

    Plasmid

    #159686

    Purpose

    Mammalian protein expression of NRAS HVR (Hypervariable region)-Halotag fusion

    Depositor

    Dominic Esposito

    Article

    Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

    Insert

    Halotag-NRasHVR (NRAS Human)
    

    Tags

    Halotag

    Expression

    Mammalian

    Available Since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge