We narrowed to 12,546 results for: NUC
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Plasmid#71134PurposeExpression of Acetobacter aceti 1023 PurE (H89N mutant) in E. coliDepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationchanges histidine-89 to asparagine; additional N-…PromoterT7Available sinceFeb. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK320
Plasmid#71144PurposeExpression of Acetobacter aceti 1023 PurE (H59S mutant) in E. coliDepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationchanges histidine-59 to serine; additional N-term…PromoterT7Available sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK319
Plasmid#71143PurposeExpression of Acetobacter aceti 1023 PurE (H59A mutant) in E. coliDepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationchanges histidine-59 to alanine; additional N-ter…PromoterT7Available sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK318
Plasmid#71142PurposeExpression of Acetobacter aceti 1023 PurE (H59Q mutant) in E. coliDepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationchanges histidine-59 to glutamine; additional N-t…PromoterT7Available sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK315
Plasmid#71141PurposeExpression of Acetobacter aceti 1023 PurE (H89G mutant) in E. coliDepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationchanges histidine-89 to glycine; additional N-ter…PromoterT7Available sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK314
Plasmid#71140PurposeExpression of Acetobacter aceti 1023 PurE (H89V mutant) in E. coliDepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationchanges histidine-89 to valine; additional N-term…PromoterT7Available sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK279
Plasmid#71135PurposeExpression of Acetobacter aceti 1023 PurE (H89D mutant) in E. coliDepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationchanges histidine-89 to aspartate; additional N-t…PromoterT7Available sinceJan. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK283
Plasmid#71139PurposeExpression of Acetobacter aceti 1023 PurE (H59N mutant) in E. coliDepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationchanges histidine-59 to asparagine; additional N-…PromoterT7Available sinceJan. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK282
Plasmid#71138PurposeExpression of Acetobacter aceti 1023 PurE (H59D mutant) in E. coliDepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationchanges histidine-59 to aspartate; additional N-t…PromoterT7Available sinceJan. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK281
Plasmid#71137PurposeExpression of Acetobacter aceti 1023 PurE (H59F mutant) in E. coliDepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationchanges histidine-59 to phenylalanine; additional…PromoterT7Available sinceJan. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK280
Plasmid#71136PurposeExpression of Acetobacter aceti 1023 PurE (H89F mutant) in E. coliDepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationchanges histidine-89 to phenylalanine; additional…PromoterT7Available sinceJan. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSJ1321 (pRS315-NOP1pr-GFP11-mCherry-PUS1)
Plasmid#86413Purposenuclear split GFP11 reporter with mCherryDepositorInsertNop1pr-GFP11-mCherry-PUS1
UseTagsGFP11 and mCherryExpressionYeastMutationPromoterNOP1Available sinceFeb. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSJ1679 (pRS315-NOP1pr-GFP11-PUS1)
Plasmid#86414Purposenuclear split GFP11 reporter without mCherryDepositorInsertNOP1pr-GFP11-PUS1
UseTagsGFP11 and mCherryExpressionYeastMutationPromoterNOP1prAvailable sinceFeb. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDN41
Plasmid#61344PurposemCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS9/PKIt NES)DepositorInsertmCherry
UseTagsGS linker-AsLov2-biNLS9 and PKIt NESExpressionMammalianMutationPromoterCMVAvailable sinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-TORCAR-NES
Plasmid#175015PurposeCytosol-targeted TORCAR.DepositorInsertTORCAR-NES
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDN77
Plasmid#61347PurposemCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS2/IkBα NES)DepositorInsertmCherry
UseTagsGS linker-AsLov2-biNLS2 and NES IkBaExpressionMammalianMutationPromoterCMVAvailable sinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
NET31 mRFP-N2 (490)
Plasmid#61984Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertNET31 (TMEM209 Human)
UseTagsmRFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCLASP_mScarlet_CLASP_RGS2membrane
Plasmid#133086PurposePlasmid contains mScarlet with CLASP. This consists of the plasma membrane anchor (pm-LOVTRAP) and the cargo (Zdk-mScarlet-yeLANS). CLASP modulates nuclear localization of mScarlet in response to blDepositorInsertmScarlet-CLASP
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pK7WGF2::hCas9
Plasmid#46965PurposeExpesses the human codon usage Cas9 nuclease (Mali et al. Science 339, 823-826, 2013) with an N-terminal GFP tag from the 35S promoter in the plant tissueDepositorInserthCas9
UseCRISPR; Plant expressionTagseGFPExpressionMutationPromoter35SAvailable sinceAug. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCAG-AsCas12a-Plus-mCherry
Plasmid#191231PurposeTo express the AsCas12a nuclease variant "AsCas12a-Plus" that enhanced the editing activity and specificityDepositorInsertAsCas12a-Plus-P2A-mCherry
UseCRISPRTagsExpressionMutationE174R, R951K, R955APromoterAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only