We narrowed to 13,284 results for: SHI

1. pMT1461

   Plasmid

   #228319

   Purpose

   Doxycycline-regulatable ALX-0081 expression

   Depositor

   Jun Ishii

   Article

   Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

   Insert

   MFalpha(2xAdv)-ALX-0081-3A-FLAG-His
   

   Use

   Affinity Reagent/ Antibody

   Tags

   AAA linker followed by FLAG-His6 and MF_ secretio…

   Expression

   Yeast

   Mutation

   Promoter

   Doxycycline-regulatable synthetic promoter (KpTc7…

   Available since

   March 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPAP_scFv_V50A

   Plasmid

   #183734

   Purpose

   Expresses anti-lysozyme scFv antibody with V50A mutation on MFalpha signal

   Depositor

   Jun Ishii

   Article

   Ito et al Commun Biol. 2022 Jun 8;5(1):561. doi: 1

   Insert

   Anti-lysozyme scFv
   

   Use

   Tags

   His6-tag

   Expression

   Yeast

   Mutation

   V50A mutation in MFalpha signal

   Promoter

   AOX1 promoter

   Available since

   Feb. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pT2AL200R150G-EGFP-LC3B-RFP-LC3BΔG

   Plasmid

   #168998

   Purpose

   Expresses GFP-LC3-RFP-LC3ΔG in zebrafish to measure autophagic flux

   Depositor

   Noboru Mizushima

   Article

   Kaizuka et al Mol Cell. 2016 Nov 17;64(4):835-849. doi

   Insert

   microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
   

   Use

   Unspecified

   Tags

   EGFP and mRFP1

   Expression

   Mutation

   Promoter

   Available since

   April 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCold-I-Dsup

   Plasmid

   #90021

   Purpose

   To express His-tagged Dsup in bacteria

   Depositor

   Takekazu Kunieda

   Article

   Hashimoto et al Nat Commun. 2016 Sep 20;7:12808. doi: 10

   Insert

   Dsup
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   cspA

   Available since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHes1(467)-luc

   Plasmid

   #41723

   Depositor

   Raphael Kopan, Ryoichiro Kageyama

   Article

   Nishimura et al Genomics. 1998 Apr 1;49(1):69-75.

   Insert

   Hes1 Promoter (-467 to +46) (Hes1 Mouse)
   

   Use

   Luciferase

   Tags

   Firefly luciferase

   Expression

   Mammalian

   Mutation

   Contains the murine Hes1 promoter (-467 to +46)

   Promoter

   Hes1 promoter fragment (-467 to +46)

   Available since

   March 20, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLKO.1-shDsup

   Plasmid

   #90024

   Purpose

   To establish Dsup knock down cells

   Depositor

   Takekazu Kunieda

   Article

   Hashimoto et al Nat Commun. 2016 Sep 20;7:12808. doi: 10

   Insert

   Dsup
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYI2165

   Plasmid

   #228347

   Purpose

   2,4-Diacetylphloroglucinol (DAPG)-regulatable ALX-0171 expression

   Depositor

   Jun Ishii

   Article

   Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

   Insert

   MFalpha(2xAdv)-LE-ALX-0171-3A-FLAG-His
   

   Use

   Affinity Reagent/ Antibody

   Tags

   AAA linker followed by FLAG-His6 and MF_ secretio…

   Expression

   Yeast

   Mutation

   Promoter

   2,4-Diacetylphloroglucinol-regulatable synthetic …

   Available since

   March 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYI2216

   Plasmid

   #228349

   Purpose

   2,4-Diacetylphloroglucinol (DAPG)-regulatable ALX-0081 expression

   Depositor

   Jun Ishii

   Article

   Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

   Insert

   MFalpha(2xAdv)-LE-ALX-0081-3A-FLAG-His
   

   Use

   Affinity Reagent/ Antibody

   Tags

   AAA linker followed by FLAG-His6 and MF_ secretio…

   Expression

   Yeast

   Mutation

   Promoter

   2,4-Diacetylphloroglucinol-regulatable synthetic …

   Available since

   March 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3-GFP-LC3-RFP-LC3ΔG

   Plasmid

   #168997

   Purpose

   Expresses GFP-LC3-RFP-LC3ΔG in mammalian cells and zebrafish to measure autophagic flux

   Depositor

   Noboru Mizushima

   Article

   Kaizuka et al Mol Cell. 2016 Nov 17;64(4):835-849. doi

   Insert

   microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
   

   Use

   Tags

   EGFP and mRFP1

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMRX-IP-GFP-LC3-RFP

    Plasmid

    #84573

    Purpose

    Expresses GFP-LC3-RFP in mammalian cells to measure autophagic flux

    Depositor

    Noboru Mizushima

    Article

    Kaizuka et al Mol Cell. 2016 Nov 17;64(4):835-849. doi

    Insert

    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    

    Use

    Retroviral

    Tags

    EGFP and mRFP1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHes1(467 RBPj(-))-luc

    Plasmid

    #43805

    Depositor

    Ryoichiro Kageyama

    Article

    Nishimura et al Genomics. 1998 Apr 1;49(1):69-75.

    Insert

    Hes1 Promoter (-467 to +46) RBPj(-) (Hes1 Mouse)
    

    Use

    Luciferase

    Tags

    Firefly luciferase

    Expression

    Mammalian

    Mutation

    Contains the murine Hes1 promoter (-467 to +46), …

    Promoter

    Hes1 promoter fragment (-467 to +46) (RBPj-)

    Available since

    April 5, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pAcGFP1-N1-Dsup

    Plasmid

    #90020

    Purpose

    To express GFP-tagged Dsup in mammalian cells transiently

    Depositor

    Takekazu Kunieda

    Article

    Hashimoto et al Nat Commun. 2016 Sep 20;7:12808. doi: 10

    Insert

    Dsup
    

    Use

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAcGFP1-N1-Dsup-C

    Plasmid

    #90022

    Purpose

    To express GFP-tagged C-terminal region of Dsup in mammalian cells transiently

    Depositor

    Takekazu Kunieda

    Article

    Hashimoto et al Nat Commun. 2016 Sep 20;7:12808. doi: 10

    Insert

    Dsup
    

    Use

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    C-terminal region alone

    Promoter

    CMV

    Available since

    May 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMRX-IP-GFP-LC3-RFP-LC3ΔG

    Plasmid

    #84572

    Purpose

    Expresses GFP-LC3-RFP-LC3ΔG in mammalian cells to measure autophagic flux

    Depositor

    Noboru Mizushima

    Article

    Kaizuka et al Mol Cell. 2016 Nov 17;64(4):835-849. doi

    Insert

    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    

    Use

    Retroviral

    Tags

    EGFP and mRFP1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCXN2KS-Dsup

    Plasmid

    #90019

    Purpose

    To establish stable line expressing Dsup in constitutive manner

    Depositor

    Takekazu Kunieda

    Article

    Hashimoto et al Nat Commun. 2016 Sep 20;7:12808. doi: 10

    Insert

    Dsup
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAcGFP1-N1-Dsup-deltaC

    Plasmid

    #90023

    Purpose

    To express GFP-tagged Dsup lacking C-terminal region in mammalian cells transiently

    Depositor

    Takekazu Kunieda

    Article

    Hashimoto et al Nat Commun. 2016 Sep 20;7:12808. doi: 10

    Insert

    Dsup
    

    Use

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    Deletion of C-terminal region

    Promoter

    CMV

    Available since

    Aug. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. Fucci(CA) hCdt1-iRFP hGeminin-TagBFP2

    Plasmid

    #190181

    Purpose

    Fluorescent reporter vector to visualize all of cell cycle phases.

    Depositor

    Toshiro Sato

    Article

    Ohta et al Nature. 2022 Jul 7. pii: 10.1038/s41586-

    Inserts

    iRFP
    hCdt1(1/100)Cy(-) (Cdt1 Synthetic)
    TagBFP2
    hGeminin(1/110)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. Fucci(CA) hCdt1-iRFP hGeminin-iRFP

    Plasmid

    #190182

    Purpose

    Fluorescent reporter vector for visualization of G0 and other cell cycle phases.

    Depositor

    Toshiro Sato

    Article

    Ohta et al Nature. 2022 Jul 7. pii: 10.1038/s41586-

    Inserts

    iRFP
    hCdt1(1/100)Cy(-) (Cdt1 Synthetic)
    iRFP
    hGeminin(1/110)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAGGS-FuG-C

    Plasmid

    #67512

    Purpose

    This is an envelope gene encoding plasmid to make retrogradely lentiviral vector. If you use this plasmid, you can make type C envelope coating virus particle with NeuRet.

    Depositor

    Kazuto Kobayashi

    Article

    Kato et al Hum Gene Ther. 2011 Dec;22(12):1511-23.

    Insert

    Fusion Glycoprotein type C
    

    Use

    Lentiviral

    Tags

    Fusion protein of Vesicular stomatitis Indiana vi…

    Expression

    Mutation

    Promoter

    CAGGS

    Available since

    July 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-D12L

    Plasmid

    #89161

    Purpose

    Express vaccinia virus D12L in mammalian cells

    Depositor

    Takeshi Kobayashi

    Article

    Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

    Insert

    Vaccinia virus D12L
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promotor

    Available since

    April 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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