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We narrowed to 6,025 results for: plasmid dna

Showing: 1381 - 1400 of 6025 results

  1. CMV+ plasmid

    Plasmid
    #169740
    Purpose
    Unregulated "open-loop" CMV promoter plasmid. This plasmid encodes eGFP to report circuit output levels. This plasmid has additional sequences in the UTRs to increase gene expression levels.
    Depositor
    Francois St-Pierre
    Article
    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:
    Insert
    eGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    pCMV-tetO2
    Available since
    June 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    169740-plasmid-map-sequence-id-334212

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  2. BPK3258 - human expression plasmid for eSpCas9(1.1)

    Plasmid
    #101176
    Purpose
    Human expression plasmid for SpCas9 eSpCas9(1.1) variant: CMV-T7-hSpCas9-eSpCas9(1.1)(K848A, K1003A, R1060A)-NLS(SV40)-3xFLAG
    Depositor
    Keith Joung, Jennifer Doudna
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    hSpCas9-eSpCas9(1.1)(K848A/K1003A/R1060A)
    Use
    Tags
    3x FLAG and NLS (SV40)
    Expression
    Mammalian
    Mutation
    K848A/K1003A/R1060A
    Promoter
    CMV
    Available since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    101176-plasmid-map-sequence-id-193999

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  3. BPK4387 - human expression plasmid for SpCas9 Cluster 5

    Plasmid
    #101191
    Purpose
    Human expression plasmid for SpCas9 Cluster 5 variant: CMV-T7-hSpCas9-Cluster5(K918A, V922A, R925A)-NLS(SV40)-3xFLAG
    Depositor
    Keith Joung, Jennifer Doudna
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    hSpCas9-Cluster5(K918A/V922A/R925A)
    Use
    Tags
    3x FLAG and NLS (SV40)
    Expression
    Mammalian
    Mutation
    K918A/V922A/R925A
    Promoter
    CMV
    Available since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    101191-plasmid-map-sequence-id-194254

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  4. MMW3645 - human expression plasmid for SpCas9 Cluster 3

    Plasmid
    #101187
    Purpose
    Human expression plasmid for SpCas9 Cluster 3 variant: CMV-T7-hSpCas9-Cluster3(T657A, G658A, W659A, R661A)-NLS(SV40)-3xFLAG
    Depositor
    Keith Joung, Jennifer Doudna
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    hSpCas9-Cluster3(T657A/G658A/W659A/R661A)
    Use
    Tags
    3x FLAG and NLS (SV40)
    Expression
    Mammalian
    Mutation
    T657A/G658A/W659A/R661A
    Promoter
    CMV
    Available since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    101187-plasmid-map-sequence-id-194250

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  5. MMW3770 - human expression plasmid for SpCas9 Cluster 4

    Plasmid
    #101189
    Purpose
    Human expression plasmid for SpCas9 Cluster 4 variant: CMV-T7-hSpCas9-Cluster4(F491A, M495A, T496A, N497A)-NLS(SV40)-3xFLAG
    Depositor
    Keith Joung, Jennifer Doudna
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    hSpCas9-Cluster4(F491A/M495A/T496A/N497A)
    Use
    Tags
    3x FLAG and NLS (SV40)
    Expression
    Mammalian
    Mutation
    F491A/M495A/T496A/N497A
    Promoter
    CMV
    Available since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    101189-plasmid-map-sequence-id-194252

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  6. MMW2993 - human expression plasmid for SpCas9 Cluster 2

    Plasmid
    #101185
    Purpose
    Human expression plasmid for SpCas9 Cluster 2 variant: CMV-T7-hSpCas9-Cluster2(G582A, V583A, E584A, D585A, N588A)-NLS(SV40)-3xFLAG
    Depositor
    Keith Joung, Jennifer Doudna
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    hSpCas9-Cluster2(G582A/V583A/E584A/D585A/N588A)
    Use
    Tags
    3x FLAG and NLS (SV40)
    Expression
    Mammalian
    Mutation
    G582A/V583A/E584A/D585A/N588A
    Promoter
    CMV
    Available since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    101185-plasmid-map-sequence-id-194249

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  8. pJSC173 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9) variant

    Plasmid
    #101218
    Purpose
    Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9) variant
    Depositor
    Keith Joung, Jennifer Doudna
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant N692A/M694A/Q695A/H698A
    Use
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    N692A, M694A, Q695A and H698A
    Promoter
    T7
    Available since
    Nov. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    101218-plasmid-map-sequence-id-198035

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  9. pJSC272 - Bacterial expression plasmid for SpCas9 Cluster 5 + Q926A variant

    Plasmid
    #101236
    Purpose
    Bacterial expression plasmid for SpCas9 Cluster 5 + Q926A variant
    Depositor
    Keith Joung, Jennifer Doudna
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant K918A/V922A/R925A/Q926A
    Use
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    K918A, V922A, R925A and Q926A
    Promoter
    T7
    Available since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    101236-plasmid-map-sequence-id-197520

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  10. pJSC236 - Bacterial expression plasmid for SpCas9 Cluster 4 + Q926A variant

    Plasmid
    #101235
    Purpose
    Bacterial expression plasmid for SpCas9 Cluster 4 + Q926A variant
    Depositor
    Keith Joung, Jennifer Doudna
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant F491A/M495A/T496A/N497A/Q926A
    Use
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    F491A, M495A, T496A, N497A and Q926A
    Promoter
    T7
    Available since
    Nov. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    101235-plasmid-map-sequence-id-198037

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  11. pJSC239 - Bacterial expression plasmid for SpCas9 Cluster 3 + Q926A variant

    Plasmid
    #101234
    Purpose
    Bacterial expression plasmid for SpCas9 Cluster 3 + Q926A variant
    Depositor
    Keith Joung, Jennifer Doudna
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant T657A/G658A/W659A/R661A/Q926A
    Use
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    T657A, G658A, W659A, R661A and Q926A
    Promoter
    T7
    Available since
    Nov. 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    101234-plasmid-map-sequence-id-198016

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  12. pJSC269 - Bacterial expression plasmid for SpCas9 Cluster 1 + Q926A variant

    Plasmid
    #101220
    Purpose
    Bacterial expression plasmid for SpCas9 Cluster 1 + Q926A variant
    Depositor
    Keith Joung, Jennifer Doudna
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant N692A/M694A/Q695A/H698A/Q926A
    Use
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    N692A, M694A, Q695A, H698A and Q926A
    Promoter
    T7
    Available since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    101220-plasmid-map-sequence-id-197535

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  13. pJSC197 - Bacterial expression plasmid for SpCas9 Cluster 2 + Q926A variant

    Plasmid
    #101232
    Purpose
    Bacterial expression plasmid for SpCas9 Cluster 2 + Q926A variant
    Depositor
    Keith Joung, Jennifer Doudna
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant G528A/V583A/E584A/D585A/N588A/Q926A
    Use
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    G528A, V583A, E584A, D585A, N588A and Q926A
    Promoter
    T7
    Available since
    Oct. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    101232-plasmid-map-sequence-id-197212

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  16. BPK4410 - human expression plasmid for SpCas9 Cluster 1 (HypaCas9)

    Plasmid
    #101178
    Purpose
    Human expression plasmid for SpCas9 Cluster 1 variant (HypaCas9): CMV-T7-hSpCas9-Cluster1(N692A, M694A, Q695A, H698A)-NLS(SV40)-3xFLAG
    Depositor
    Keith Joung, Jennifer Doudna
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    hSpCas9-Cluster1(N692A/M694A/Q695A/H698A)
    Use
    Tags
    3x FLAG and NLS (SV40)
    Expression
    Mammalian
    Mutation
    N692A/M694A/Q695A/H698A
    Promoter
    CMV
    Available since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    101178-plasmid-map-sequence-id-219907

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  17. BPK4393 - human expression plasmid for SpCas9 Cluster 5 + Q926A

    Plasmid
    #101192
    Purpose
    Human expression plasmid for SpCas9 Cluster 5 + Q926A variant: CMV-T7-hSpCas9-Cluster5+Q926A(K918A, V922A, R925A, Q926A)-NLS(SV40)-3xFLAG
    Depositor
    Keith Joung, Jennifer Doudna
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    hSpCas9-Cluster5+Q926A(K918A/V922A/R925A/Q926A)
    Use
    Tags
    3x FLAG and NLS (SV40)
    Expression
    Mammalian
    Mutation
    K918A/V922A/R925A/Q926A
    Promoter
    CMV
    Available since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    101192-plasmid-map-sequence-id-194255

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  18. MMW3629 - human expression plasmid for SpCas9 Cluster 3 + Q926A

    Plasmid
    #101188
    Purpose
    Human expression plasmid for SpCas9 Cluster 3 + Q926A variant: CMV-T7-hSpCas9-Cluster3+Q926A(T657A, G658A, W659A, R661A, Q926A)-NLS(SV40)-3xFLAG
    Depositor
    Keith Joung, Jennifer Doudna
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    hSpCas9-Cluster3+Q926A(T657A/G658A/W659A/R661A/Q926A)
    Use
    Tags
    3x FLAG and NLS (SV40)
    Expression
    Mammalian
    Mutation
    T657A/G658A/W659A/R661A/Q926A
    Promoter
    CMV
    Available since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    101188-plasmid-map-sequence-id-194251

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  19. MMW3759 - human expression plasmid for SpCas9 Cluster 4 + Q926A

    Plasmid
    #101190
    Purpose
    Human expression plasmid for SpCas9 Cluster 4 + Q926A variant: CMV-T7-hSpCas9-Cluster4+Q926A(F491A, M495A, T496A, N497A, Q926A)-NLS(SV40)-3xFLAG
    Depositor
    Keith Joung, Jennifer Doudna
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    hSpCas9-Cluster4+Q926A(F491A/M495A/T496A/N497A/Q926A)
    Use
    Tags
    3x FLAG and NLS (SV40)
    Expression
    Mammalian
    Mutation
    F491A/M495A/T496A/N497A/Q926A
    Promoter
    CMV
    Available since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    101190-plasmid-map-sequence-id-194253

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  20. MMW3001 - human expression plasmid for SpCas9 Cluster 2 + Q926A

    Plasmid
    #101184
    Purpose
    Human expression plasmid for SpCas9 Cluster 2 + Q926A variant: CMV-T7-hSpCas9-Cluster2+Q926A(G582A, V583A, E584A, D585A, N588A, Q926A)-NLS(SV40)-3xFLAG
    Depositor
    Keith Joung, Jennifer Doudna
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    hSpCas9-Cluster2+Q926A(G582A/V583A/E584A/D585A/N588A/Q926A)
    Use
    Tags
    3x FLAG and NLS (SV40)
    Expression
    Mutation
    G582A/V583A/E584A/D585A/N588A/Q926A
    Promoter
    CMV
    Available since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    101184-plasmid-map-sequence-id-194248

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Showing: 1381 - 1400 of 6025 results