We narrowed to 3,198 results for: ER

1. pcDNA-HisFlag-rUSP19ER-CAmut

   Plasmid

   #155237

   Purpose

   for mammalian overexpression of the mutated ER isoform of rat USP19

   Depositor

   Simon Wing

   Article

   Lu et al Mol Cell Biol. 2009 Jan;29(2):547-58. do

   Insert

   USP19 (ER isoform) (Usp19 Rat)
   

   Use

   Tags

   6XHis-3XFlag

   Expression

   Mammalian

   Mutation

   Cys(545) to Ala

   Promoter

   CMV

   Available since

   Oct. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLz26_CsoS2_N1-N2_GFP

   Plasmid

   #140851

   Purpose

   His-tagged first two N-peptides of NTD fused to Superfolder GFP ([N1-N2]-GFP) used for MST, pET-14b backbone

   Depositor

   David Savage

   Article

   Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

   Insert

   CsoS2 NTD repeats 1-2 fused to GFP
   

   Use

   Tags

   Hexahistidine tag, Strep-Tag II, and Superfolder …

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX-4T-3-cytXlATL2-R232Q

   Plasmid

   #124064

   Purpose

   Control protein. The R232Q mutation abolishes dimerization activity, so this construct is inactive compared to wild type cytATL

   Depositor

   Tom Rapoport

   Article

   Wang et al J Cell Biol. 2013 Dec 9;203(5):801-14. d

   Insert

   cytXlATL2-R232Q
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. CoxVIIIx2-CeNL(Ca2+)_19μ-pcDNA3

   Plasmid

   #111923

   Purpose

   Cyan color luminescent indicator for calcium signaling.Mitochondrial targeting signals of subunit VIII of human cytochrome c oxidase (CoxVIII) were fused at the N-terminus of CeNL(Ca2+)_19μ

   Depositor

   Takeharu Nagai

   Article

   Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

   Insert

   CeNL(Ca2+)_19μ
   

   Use

   Tags

   CoxVIIIx2

   Expression

   Mammalian

   Mutation

   E67D, F92W, E104D, D133E at CaM

   Promoter

   CMV

   Available since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. CoxVIIIx2-CeNL(Ca2+)_1.2μ-pcDNA3

   Plasmid

   #111922

   Purpose

   Cyan color luminescent indicator for calcium signaling.Mitochondrial targeting signals of subunit VIII of human cytochrome c oxidase (CoxVIII) were fused at the N-terminus of CeNL(Ca2+)_1.2μ

   Depositor

   Takeharu Nagai

   Article

   Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

   Insert

   CeNL(Ca2+)_1.2μ
   

   Use

   Tags

   CoxVIIIx2

   Expression

   Mammalian

   Mutation

   E67D, E104D, D133E at CaM

   Promoter

   CMV

   Available since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-Ubl4A K48R

   Plasmid

   #61809

   Purpose

   expresses mutated Ubl4A with K48R, with a C-terminal FLAG tag

   Depositor

   Yihong Ye

   Article

   Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

   Insert

   UBL4A (UBL4A Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   K48R

   Promoter

   CMV

   Available since

   Feb. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pBS cSugarCrisp (CT#708)

   Plasmid

   #13956

   Depositor

   Cliff Tabin

   Article

   Smith et al Mech Dev. 2001 Apr . 102(1-2):223-6.

   Insert

   SugarCrisp (PI15 Chicken)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

8. pBS hSugarCrisp (CT#592)

   Plasmid

   #13966

   Depositor

   Cliff Tabin

   Article

   Smith et al Mech Dev. 2001 Apr . 102(1-2):223-6.

   Insert

   SugarCrisp (PI15 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

9. pFSW Doc2alpha D291N mutant

   Plasmid

   #128813

   Purpose

   To make virus expressing Doc2alpha D291 mutant

   Depositor

   Edwin Chapman

   Article

   Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

   Insert

   Doc2alpha (Doc2a Rat)
   

   Use

   Lentiviral

   Tags

   IRES-EGFP

   Expression

   Mutation

   D291N

   Promoter

   hSyn (human synapsin I promoter)

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFSW Doc2beta D303N mutant

    Plasmid

    #128815

    Purpose

    To make virus expressing Doc2beta D303N mutant

    Depositor

    Edwin Chapman

    Article

    Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

    Insert

    Doc2beta (Doc2b Rat)
    

    Use

    Lentiviral

    Tags

    IRES-EGFP

    Expression

    Mutation

    D303N

    Promoter

    hSyn (human synapsin I promoter)

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFSW Doc2alpha Halotag

    Plasmid

    #128817

    Purpose

    To make virus expressing Doc2alpha Halotag

    Depositor

    Edwin Chapman

    Article

    Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

    Insert

    Doc2alpha (Doc2a Rat)
    

    Use

    Lentiviral

    Tags

    HaloTag-IRES-EGFP

    Expression

    Mutation

    Promoter

    hSyn (human synapsin I promoter)

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFSW Doc2beta Halotag

    Plasmid

    #128818

    Purpose

    To make virus expressing Doc2beta Halotag

    Depositor

    Edwin Chapman

    Article

    Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

    Insert

    Doc2beta (Doc2b Rat)
    

    Use

    Lentiviral

    Tags

    HaloTag-IRES-EGFP

    Expression

    Mutation

    Promoter

    hSyn (human synapsin I promoter)

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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13. CytERM-mKOkappa

    Plasmid

    #98832

    Purpose

    In vivo visualization of the ER (can be used for colocalization studies and the OSER assay)

    Depositor

    Dorus Gadella

    Insert

    CytERM
    

    Use

    Tags

    mKOkappa

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. CytERM_3xmTurquoise2

    Plasmid

    #112958

    Purpose

    In vivo visualization of the ER (can be used for colocalization studies and the OSER assay)

    Depositor

    Dorus Gadella

    Insert

    CytERM
    

    Use

    Tags

    3x mTurquoise2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEX-KG-D4H*-mCherry

    Plasmid

    #134604

    Purpose

    fluorescent cholesterol-binding probe

    Depositor

    Roberto Zoncu

    Article

    Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218

    Insert

    D4H*
    

    Use

    Tags

    GST and mCherry

    Expression

    Bacterial

    Mutation

    Y415A and A463W

    Promoter

    Available since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLentiCRISPR-E

    Plasmid

    #78852

    Purpose

    Introduce sgRNA into a lentiviral vector (LentiCRISPR V2) which contains eSpCas9 and puromycin cassette

    Depositor

    Phillip Abbosh

    Article

    Sikder et al Mol Cancer Res. 2020 Dec 3. pii: 1541-77

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Cas9-P2A-Puro

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. ins:MCS2; cryaa:RFP

    Plasmid

    #110286

    Purpose

    A zebrafish insulin promoter containing vector with a second multiple cloning sites. Contains red eye marker, and is flanked with I-SceI sites.

    Depositor

    Nikolay Ninov

    Article

    Singh et al Nat Commun. 2017 Sep 22;8(1):664. doi: 1

    Type

    Empty backbone

    Use

    Zebrafish

    Tags

    Expression

    Mutation

    Promoter

    insulin

    Available since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. mCh-Drp1

    Plasmid

    #49152

    Purpose

    mammalian expression of Drp1 fused to mCherry

    Depositor

    Gia Voeltz

    Article

    Friedman et al Science. 2011 Oct 21;334(6054):358-62. d

    Insert

    Drp1 (DNM1L Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAGGS_cAMPFIRE-L

    Plasmid

    #182283

    Purpose

    Mammalian expression of the cAMP sensor cAMPFIRE-L under a CAG promotor w/ WPRE translation enhancer.

    Depositor

    Haining Zhong

    Article

    Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

    Insert

    cAMPFIRE-L (cAMP sensor)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAGGS_cAMPFIRE-M

    Plasmid

    #182284

    Purpose

    Mammalian expression of the cAMP sensor cAMPFIRE-M under a CAG promotor w/ WPRE translation enhancer.

    Depositor

    Haining Zhong

    Article

    Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

    Insert

    cAMPFIRE-M (cAMP sensor)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge