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pX260-U6-DR-BB-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro

Plasmid

#42229

Purpose

This plasmid separately encodes a human codon-optimized SpCas9, a tracrRNA and customizable crRNA.

Depositor

Feng Zhang

Article

Cong et al Science. 2013 Jan 3.

Insert

humanized S. pyogenes Cas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Available Since

March 7, 2013

Availability

Academic Institutions and Nonprofits only

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pX261-U6-DR-hEmx1-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro

Plasmid

#42337

Purpose

Dual expression plasmid of human codon-optimized SpCas9 and a gRNA to the human Emx1 locus, can be used to test SpCas9 cleavage in cell lines of choice.

Depositor

Feng Zhang

Article

Cong et al Science. 2013 Jan 3.

Insert

humanized S. pyogenes Cas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Available Since

Feb. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pDF0338 pAAV U6-HvsCas7-11 DR Rev-BpiI golden gate backbone dual vector

Plasmid

#172514

Purpose

Encodes HvsCas7-11 DR and golden gate site for spacer clonings in an AAV backbone with U6 promoter

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

pAAV U6-HvsCas7-11 DR Rev-BpiI golden gate backbone dual vector

Use

AAV

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pRS314_U6_A34G_A35G

Plasmid

#81232

Purpose

U6 telestem mutant (A34G/A35G) for in vivo studies

Depositor

Aaron Hoskins

Article

Rodgers et al Nucleic Acids Res. 2016 Dec 15;44(22):10

Insert

U6 RNA with A34G/A35G mutation (snR6 Budding Yeast)

Expression

Yeast

Mutation

U6 telestem mutant (A34G/A35G)

Available Since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pRS314_U6_G96C_A97C

Plasmid

#81235

Purpose

U6 telestem mutant (G96C/A97C) for in vivo studies

Depositor

Aaron Hoskins

Article

Rodgers et al Nucleic Acids Res. 2016 Dec 15;44(22):10

Insert

U6 RNA with G96C/A97C mutation (snR6 Budding Yeast)

Expression

Yeast

Mutation

U6 telestem mutant (G96C/A97C)

Available Since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pRS314_U6_A34G_A35G

Plasmid

#81232

Purpose

U6 telestem mutant (A34G/A35G) for in vivo studies

Depositor

Aaron Hoskins

Article

Rodgers et al Nucleic Acids Res. 2016 Dec 15;44(22):10

Insert

U6 RNA with A34G/A35G mutation (snR6 Budding Yeast)

Expression

Yeast

Mutation

U6 telestem mutant (A34G/A35G)

Available Since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pRS314_U6_G96C_A97C

Plasmid

#81235

Purpose

U6 telestem mutant (G96C/A97C) for in vivo studies

Depositor

Aaron Hoskins

Article

Rodgers et al Nucleic Acids Res. 2016 Dec 15;44(22):10

Insert

U6 RNA with G96C/A97C mutation (snR6 Budding Yeast)

Expression

Yeast

Mutation

U6 telestem mutant (G96C/A97C)

Available Since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pX334-U6-DR-BB-DR-Cbh-NLS-hSpCas9n(D10A)-NLS-H1-shorttracr-PGK-puro

Plasmid

#42333

Purpose

This plasmid separately encodes a human codon-optimized SpCas9 nickase, a tracrRNA and customizable crRNA.

Depositor

Feng Zhang

Article

Cong et al Science. 2013 Jan 3.

Insert

humanized S. pyogenes Cas9 (D10A) nickase

Use

CRISPR

Tags

HA

Expression

Mammalian

Mutation

D10A nickase-converting mutation

Available Since

March 7, 2013

Availability

Academic Institutions and Nonprofits only

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pRC49-U6-DR-crRNA-BsmbI(x2)-6T; EFS-Puro-2A-Fluc-2A-EGFP_NLS-WPRE

Plasmid

#123363

Purpose

Lentiviral vector with empty U6 cassette containing LbCpf1 direct repeat and U6 terminator, with constitutive expression of puromycin resistance, Firefly luciferase, and nuclear EGFP.

Depositor

Sidi Chen

Article

Chow et al Nat Methods. 2019 May;16(5):405-408. doi

Insert

EGFP-NLS

Use

CRISPR, Lentiviral, and Luciferase

Expression

Mammalian

Promoter

EFS

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-neo-TET

Plasmid

#51286

Purpose

expresses U6 RNA, using its own pol III enhancer sequence and tagged with the self splicing intron neo cassette

Depositor

Astrid Roy-Engel

Article

Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:

Insert

U6 (RNU6-1 Human)

Tags

neoTET cassette with a tetrahymena self-splicing …

Expression

Mammalian

Promoter

U6 upstream enhancer sequence

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

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pU6-neo-TET

Plasmid

#51286

Purpose

expresses U6 RNA, using its own pol III enhancer sequence and tagged with the self splicing intron neo cassette

Depositor

Astrid Roy-Engel

Article

Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:

Insert

U6 (RNU6-1 Human)

Tags

neoTET cassette with a tetrahymena self-splicing …

Expression

Mammalian

Promoter

U6 upstream enhancer sequence

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

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EPB104

Plasmid

#68369

Purpose

contains human U6 promoter

Depositor

Eric-Paul Bennett

Article

Lonowski et al Nat Protoc. 2017 Mar;12(3):581-603. doi:

Insert

U6 promoter (RNU6-1 Human)

Expression

Bacterial

Promoter

U6

Available Since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

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EPB104

Plasmid

#68369

Purpose

contains human U6 promoter

Depositor

Eric-Paul Bennett

Article

Lonowski et al Nat Protoc. 2017 Mar;12(3):581-603. doi:

Insert

U6 promoter (RNU6-1 Human)

Expression

Bacterial

Promoter

U6

Available Since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-LbCpf1-U6_FlipArray

Plasmid

#109349

Purpose

Lentiviral vector to express Cpf1 from EFS promoter, as well as a Cpf1 FlipArray from U6 promoter.

Depositor

Sidi Chen

Article

Chow et al Nat Commun. 2018 May 15;9(1):1903. doi:

Insert

U6-FlipArray

Use

CRISPR, Cre/Lox, Lentiviral, and Synthetic Biolog…

Expression

Mammalian

Promoter

U6

Available Since

July 14, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-LbCpf1-U6_FlipArray

Plasmid

#109349

Purpose

Lentiviral vector to express Cpf1 from EFS promoter, as well as a Cpf1 FlipArray from U6 promoter.

Depositor

Sidi Chen

Article

Chow et al Nat Commun. 2018 May 15;9(1):1903. doi:

Insert

U6-FlipArray

Use

CRISPR, Cre/Lox, Lentiviral, and Synthetic Biolog…

Expression

Mammalian

Promoter

U6

Available Since

July 14, 2018

Availability

Academic Institutions and Nonprofits only

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pX334-U6-DR-BB-DR-Cbh-NLS-hSpCas9n(D10A)-NLS-H1-shorttracr-PGK-puro

Plasmid

#42333

Purpose

This plasmid separately encodes a human codon-optimized SpCas9 nickase, a tracrRNA and customizable crRNA.

Depositor

Feng Zhang

Article

Cong et al Science. 2013 Jan 3.

Insert

humanized S. pyogenes Cas9 (D10A) nickase

Use

CRISPR

Tags

HA

Expression

Mammalian

Mutation

D10A nickase-converting mutation

Available Since

March 7, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pRC49-U6-DR-crRNA-BsmbI(x2)-6T; EFS-Puro-2A-Fluc-2A-EGFP_NLS-WPRE

Plasmid

#123363

Purpose

Lentiviral vector with empty U6 cassette containing LbCpf1 direct repeat and U6 terminator, with constitutive expression of puromycin resistance, Firefly luciferase, and nuclear EGFP.

Depositor

Sidi Chen

Article

Chow et al Nat Methods. 2019 May;16(5):405-408. doi

Insert

EGFP-NLS

Use

CRISPR, Lentiviral, and Luciferase

Expression

Mammalian

Promoter

EFS

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pSX6

Plasmid

#90468

Purpose

U6 wt in pSE358 (TRP1 CEN)

Depositor

Christine Guthrie

Article

Madhani et al Genes Dev. 1990 Dec;4(12B):2264-77.

Insert

snR6 (snR6 Budding Yeast)

Expression

Yeast

Promoter

native

Available Since

June 29, 2024

Availability

Academic Institutions and Nonprofits only

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pSX6

Plasmid

#90468

Purpose

U6 wt in pSE358 (TRP1 CEN)

Depositor

Christine Guthrie

Article

Madhani et al Genes Dev. 1990 Dec;4(12B):2264-77.

Insert

snR6 (snR6 Budding Yeast)

Expression

Yeast

Promoter

native

Available Since

June 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCBh-NLS_hfCas13d(RfxCas13d_N2V8)_HA_NLS-pA-U6-DR-BpiI-BpiI-pSV40-EGFP-pA-pSV40-mCherry-pA

Plasmid

#190034

Purpose

vector for encoding a human codon-optimized High-fidelity RfxCas13d (hfCas13d) driven by CBh promoter, guide RNAs compatible with RfxCas13d driven by hU6, EGFP and mCherry driven by SV40 promoters

Depositor

Huawei Tong

Article

Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103

Insert

humanized hfCas13d

Expression

Mammalian

Mutation

A134V,A140V,A141V,A143V

Promoter

CBh, SV40, hU6

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

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