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pET21a(+)-Histag-Cerulean (526)

Plasmid

#70716

Purpose

Expression vector for Cerulean purification

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

Cerulean

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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DS-ST1casN-

Plasmid

#48659

Purpose

Bacterial nuclease-null ST1 Cas9 expression

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Inserts

Cas9, nuclease-null
ST1 tracrRNA

Use

CRISPR

Mutation

D to A, DH to AA, N to A

Promoter

proC

Available Since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pED14xS-WT

Plasmid

#134804

Purpose

IPTG inducible expression of WT S tRNA scaffold tRNA

Depositor

Ahmed Badran

Article

DeBenedictis et al Nat Commun. 2021 Sep 29;12(1):5706. doi:

Insert

Wildtype S tRNA (decodes TCG)

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pET21a(+)-Histag-Citrine (525)

Plasmid

#70718

Purpose

Expression vector for Citrine purification

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

Citrine

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pED14xS-11

Plasmid

#134803

Purpose

IPTG inducible expression of S-TAGA-Evo1 qtRNA

Depositor

Ahmed Badran

Article

DeBenedictis et al Nat Commun. 2021 Sep 29;12(1):5706. doi:

Insert

Wildtype S tRNA (decodes TCG)

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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DS-TDcasN-

Plasmid

#48660

Purpose

Bacterial nuclease-null TD Cas9 expression

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Inserts

Cas9, nuclease-null
TD tracrRNA

Use

CRISPR

Mutation

D to A, DH to AA, N to A

Promoter

proC and tracrRNA promoter

Available Since

April 22, 2014

Availability

Academic Institutions and Nonprofits only

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PM-SP!TB

Plasmid

#48650

Purpose

Bacterial SP crRNA expression: targets SP to protospacer B (ACTTTAAAAGTATTCGCCAT), p15A/chloramphenicol

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Bacterial SP crRNA to prototspacer B

Use

CRISPR

Promoter

J23100 promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pED14xY-3

Plasmid

#134808

Purpose

IPTG inducible expression of Y-TAGA-Evo1 qtRNA

Depositor

Ahmed Badran

Article

DeBenedictis et al Nat Commun. 2021 Sep 29;12(1):5706. doi:

Insert

Y-TAGA-Evo1 qtRNA

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pSUPER ERbeta shRNA

Plasmid

#35561

Depositor

Harish Srinivas

Article

Zhang et al Mol Endocrinol. 2009 Feb;23(2):146-56. E

Insert

ERbeta

Use

Retroviral

Available Since

April 3, 2012

Availability

Academic Institutions and Nonprofits only
Pentaprobe PP5

Plasmid

#83330

Purpose

Pentaprobe for assesing the RNA binding potential of a protein

Depositor

Joel Mackay

Article

Bendak et al Nucleic Acids Res. 2012 Aug;40(14):e105.

Insert

PP5

Use

Rna transcription

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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