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Showing: 1421 - 1440 of 39123 results

  1. pLenti PGK mKate2-OGT 5N5A (Puro)

    Plasmid
    #154293
    Purpose
    Lentiviral vector encoding full length mKate2-2xFLAG-OGT (full length human O-GlcNAc transferase, 5N5A variant, no HCF-1 cleavage)
    Depositor
    Suzanne Walker
    Article
    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11
    Insert
    O-GlcNAc Transferase (OGT Human)
    Use
    Lentiviral
    Tags
    2x FLAG and mKate2
    Expression
    Mammalian
    Mutation
    N322A, N356A, N390A, N424A, N458A mutations; HCF-…
    Promoter
    PGK
    Available since
    Nov. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    154293-plasmid-map-sequence-id-307161

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  3. EFS-SpdCas9-Dnmt3A/3L-WT

    Plasmid
    #222497
    Purpose
    Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to WT Dnmt3A/3L followed by P2A-mCherry
    Depositor
    Isaac Hilton
    Article
    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021
    Insert
    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L engineered fusion
    Use
    Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    EFS
    Available since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    222497-plasmid-map-sequence-id-441134

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  5. pcDNA.3.1.ADRB2-NP

    Plasmid
    #134376
    Purpose
    Nanoluc complementation assay. Expression of adrenoceptor beta 2 fused at C termimus with Natural peptide (NP) of NanoLuc. Addition of signal sequence and Flag epitope at N terminus of ADRB2.
    Depositor
    Julien Hanson
    Article
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Insert
    ADRB2-NP (ADRB2 Human)
    Use
    Tags
    Flag, natural peptide of nanoluciferase, and sign…
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    T7
    Available since
    Aug. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    134376-plasmid-map-sequence-id-306396

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  9. pGL3Basic-ME.2/ApoEpromoter

    Plasmid
    #51436
    Purpose
    This plasmid is a luciferase-based reporter construct to measure the activity of the ApoE promoter fused to ME.2 enhancer
    Depositor
    Sohail Tavazoie
    Article
    Pencheva et al Cell. 2014 Feb 27;156(5):986-1001. doi:
    Insert
    APOE apolipoprotein E (APOE, ME2 Human)
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    The ME.2 enhancer is fused upstream of the ApoE g…
    Promoter
    Available since
    Feb. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    51436-plasmid-map-sequence-id-177530

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  11. EFS-SpdCas9-Dnmt3A/3L-catΔ

    Plasmid
    #222501
    Purpose
    Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant catΔ (C706A and R832E) followed by P2A-mCherry
    Depositor
    Isaac Hilton
    Article
    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021
    Insert
    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant catΔ (C706A and R832E) engineered fusion
    Use
    Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; C706A and R832…
    Promoter
    EFS
    Available since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    222501-plasmid-map-sequence-id-441220

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  12. pGL3Basic_ME.1/ApoEpromoter

    Plasmid
    #51435
    Purpose
    This plasmid is a luciferase-based reporter construct to measure the activity of the ApoE promoter fused to ME.1 enhancer
    Depositor
    Sohail Tavazoie
    Article
    Pencheva et al Cell. 2014 Feb 27;156(5):986-1001. doi:
    Insert
    ME.1+ApoE promoter -980/+(93 bp.) (APOE, ME1 Human)
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    ME.1 enhancer is fused upstream of ApoE promoter …
    Promoter
    Available since
    Feb. 27, 2014
    Availability
    Academic Institutions and Nonprofits only

  13. TRE3GV-SpdCas9-Dnmt3A/3L-WT

    Plasmid
    #222502
    Purpose
    Expresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to WT Dnmt3A/3L and hPGK promoter driven mCherry
    Depositor
    Isaac Hilton
    Article
    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021
    Inserts
    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L engineered fusion
    mCherry
    Use
    Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    TRE3GV and hPGK
    Available since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    222502-plasmid-map-sequence-id-441221

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  14. pLenti NLuc398-hLGALS8 IRES hCALCOCO2-CLuc394

    Plasmid
    #128387
    Purpose
    Expresses a split firefly luciferase reporter based on the full length, human Gal8 and CALCOCO2, which interact following endosomal disruption
    Depositor
    Craig Duvall
    Article
    Kilchrist et al ACS Sens. 2020 Jun 23. doi: 10.1021/acss
    Inserts
    NLuc398-3xGGGGS-LGALS8 (LGALS8 Synthetic, Human)
    CALCOCO2-3xGGGGS-CLuc394 (CALCOCO2 Human)
    Use
    Lentiviral, Luciferase, and Synthetic Biology
    Tags
    CLuc394 and NLuc398
    Expression
    Mammalian
    Mutation
    Promoter
    EFS and IRES
    Available since
    Jan. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    128387-plasmid-map-sequence-id-261539

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  17. pABD1031

    Plasmid
    #68004
    Purpose
    Protein co-localization of PIF1 (PIL5) via fluorescent fusion after transient expression in planta
    Depositor
    Bruce Downie
    Article
    Majee et al Proc Natl Acad Sci U S A. 2018 Apr 24;11
    Insert
    PHY-INTERACTING FACTOR 1 (PIF1) (PIL5 )
    Use
    Tags
    eYFP
    Expression
    Plant
    Mutation
    Promoter
    2X Cauliflower Mosaic Virus 35S promoter
    Available since
    Dec. 17, 2018
    Availability
    Academic Institutions and Nonprofits only

  19. pEHC

    Plasmid
    #112615
    Purpose
    promoterless expression plasmid driving multicistronic cassette H2BEmerald_p2A_HygroR_p2A_CreERt2
    Depositor
    Stefan Heller
    Article
    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18
    Inserts
    H2B-Emerald (H2BC3 Aequorea victoria, Human)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    Use
    Promoterless vector, ready for promoter for mamma…
    Tags
    Emerald
    Expression
    Mammalian
    Mutation
    Promoter
    No Promoter
    Available since
    Oct. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    112615-plasmid-map-sequence-id-226350

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  20. pABD1275

    Plasmid
    #68017
    Purpose
    PIF1 protein overproduction for purification from bacterial cells
    Depositor
    Bruce Downie
    Article
    Majee et al Proc Natl Acad Sci U S A. 2018 Apr 24;11
    Insert
    PHY-INTERACTING FACTOR 1 (PIF1) (PIL5 AT2G20180, Mustard Weed)
    Use
    Tags
    6x-His
    Expression
    Bacterial
    Mutation
    Codon Optimized for bacterial expression (GenScri…
    Promoter
    T7
    Available since
    Dec. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 1421 - 1440 of 39123 results