We narrowed to 3,143 results for: N-EGFP

1. pEGFP-Flag-stm2585_S130A

   Plasmid

   #174399

   Purpose

   Native S. Typhimurium gene stm2585 (sarA/steE) with N-terminal GFP and Flag tags and its GSK3-binding SxxxSP motif mutated to SxxxAP

   Depositor

   Dennis Ko

   Article

   Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

   Insert

   sarA
   

   Use

   Tags

   EGFP and Flag

   Expression

   Mammalian

   Mutation

   S130A

   Promoter

   Available Since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFP-Flag-stm2585_Y167F

   Plasmid

   #174398

   Purpose

   Native S. Typhimurium gene stm2585 (sarA/steE) with N-terminal GFP and Flag tags and its STAT3-binding YxxQ motif mutated to FxxQ

   Depositor

   Dennis Ko

   Article

   Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

   Insert

   sarA
   

   Use

   Tags

   EGFP and Flag

   Expression

   Mammalian

   Mutation

   Y167F

   Promoter

   Available Since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5-eGFP-FRT-T0-RNF168 delta MIU1/MIU2

   Plasmid

   #133980

   Purpose

   inducible mammalian expression vector of eGFP tagged RNF168 where both motifs interacting with ubiquitin have been deleted

   Depositor

   Daniel Durocher

   Article

   Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

   Insert

   RNF168 (RNF168 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   deletion of MIU1 and MIU2

   Promoter

   CMV

   Available Since

   Nov. 4, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. phage - ubc - nls - 2xmcp - egfp - cMycBirA*

   Plasmid

   #131133

   Purpose

   BirA* expressed as a protein fusion with MS2 coat protein (MCP) dimer and EGFP; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.

   Depositor

   Ralf Jansen

   Insert

   NLS-HA-2xMCP-EGFP-cMycBirA*
   

   Use

   Lentiviral

   Tags

   NLS, HA, cMyc (N-terminal of BirA*)

   Expression

   Mammalian

   Mutation

   BirA* = BIrA >R118G

   Promoter

   human ubiquitin C (UBC)

   Available Since

   Aug. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-T7-TadCBEd-SpCas9-P2A-EGFP (BKS327)

   Plasmid

   #223123

   Purpose

   pCMV and pT7 Human expression plasmid for TadCBEd-SpCas9 with C-term BPNLS-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized TadCBEd-SpCas9-2xUGI-P2A-EGFP
   

   Use

   CRISPR

   Tags

   2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

   Expression

   Mammalian

   Mutation

   TadA-CDd mutations; nSpCas9=D10A

   Promoter

   CMV and T7

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTol2-UAS-zArchon1-KGC-EGFP-ER2

   Plasmid

   #108427

   Purpose

   Codon-optimized Archon1 fluorescent voltage reporter in zebrafish expression plasmid

   Depositor

   Edward Boyden

   Article

   Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

   Insert

   zArchon1-KGC-EGFP-ER2
   

   Use

   Zebrafish

   Tags

   Expression

   Mutation

   Promoter

   UAS

   Available Since

   May 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTol2-UAS-zArchon2-KGC-EGFP-ER2

   Plasmid

   #108428

   Purpose

   Codon-optimized Archon2 fluorescent voltage reporter in zebrafish expression plasmid

   Depositor

   Edward Boyden

   Article

   Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

   Insert

   zArchon2-KGC-EGFP-ER2
   

   Use

   Zebrafish

   Tags

   Expression

   Mutation

   Promoter

   UAS

   Available Since

   May 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-T7-SpCas9(KRHWMR)-P2A-EGFP_(RAS3808)

   Plasmid

   #228251

   Purpose

   pCMV human expression plasmid for SpCas9 enzyme with KRHWMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized nuclease SpCas9(KRHWMR)-P2A-EGFP
   

   Use

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   SpCas9(ARGIMR)=D1135K, S1136R, G1218H, E1219W, R1…

   Promoter

   CMV and T7

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. EK0493 SFFV-mCherry-SGc(GAPDH.s1)cSG-EGFP-(3xMS2) (FLP-IN)

   Plasmid

   #191156

   Purpose

   Expression of a sensor for GAPDH 3' UTR in mammalian cells; mCherry marker, EGFP output, three MS2 sequences in 3' UTR

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   mCherry:GAPDH.s1:EGFP-3xMS2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   SFFV

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. EK0445 SFFV-mCherry-SGc(DNAJB1.s1)cSG-EGFP-(3xMS2) (FLP-IN)

    Plasmid

    #191155

    Purpose

    Expression of a sensor for human DNAJB1 (hsp40) 3' UTR in mammalian cells; mCherry marker, EGFP output, three MS2 sequences in 3' UTR

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:DNAJB1.s1:EGFP-3xMS2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. NSK025 CMV-TO-EGFP:c.637A>G(N213D; CCA>CCG)-stop-t70587 (PB)

    Plasmid

    #191166

    Purpose

    Mammalian expression of a EGFP mutant N213D derived from EK0379.

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    EGFP(N213D)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    c.637A>G(N213D; CCA>CCG)

    Promoter

    CMV-TO

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP-C1-GFP-SF3B6-FLAG

    Plasmid

    #86870

    Purpose

    To express SF3B6 with a GFP and FLAG tags at N- and C-terminus, respectively.

    Depositor

    Yihong Ye

    Article

    Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

    Insert

    SF3B6 (SF3B6 Human)
    

    Use

    Tags

    EGFP and FLAG

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP-C1-FLAG-GFP-Ubl4A

    Plasmid

    #86921

    Purpose

    To express Ubl4A tagged with FLAG-EGFP at N- terminus.

    Depositor

    Yihong Ye

    Article

    Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

    Insert

    UBL4A (UBL4A Human)
    

    Use

    Tags

    FLAG and GFP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHAGE-eGFP-OPTN E50K

    Plasmid

    #175765

    Purpose

    Lentiviral vector expressing N-terminally tagged eGFP-OPTN E50K

    Depositor

    Wade Harper

    Article

    Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

    Insert

    OPTN (OPTN Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    E50K

    Promoter

    Available Since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHAGE-eGFP-OPTN S513A

    Plasmid

    #175764

    Purpose

    Lentiviral vector expressing N-terminally tagged eGFP-OPTN S513A

    Depositor

    Wade Harper

    Article

    Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

    Insert

    OPTN (OPTN Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    S513A

    Promoter

    Available Since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHAGE-eGFP-OPTN D474N

    Plasmid

    #175762

    Purpose

    Lentiviral vector expressing N-terminally tagged eGFP-OPTN D474N

    Depositor

    Wade Harper

    Article

    Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

    Insert

    OPTN (OPTN Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    D474N

    Promoter

    Available Since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. TMEM16F-I521K-peGFP-N1

    Plasmid

    #228588

    Purpose

    Expresses TMEM16F-I521K-peGFP in mammalian cells

    Depositor

    Huanghe Yang

    Article

    Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

    Insert

    TMEM16F/anoctamin 6 (Ano6), transcript variant 2 (Ano6 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    I521K plus a 3 amino acid N-terminal truncation (…

    Promoter

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. TMEM16F-I611K-peGFP-N1

    Plasmid

    #228581

    Purpose

    Expresses mouse TMEM16F-I611K-eGFP in mammalian cells

    Depositor

    Huanghe Yang

    Article

    Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

    Insert

    TMEM16F/anoctamin 6 (Ano6), transcript variant 2 (Ano6 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    I611K plus a 3 amino acid N-terminal truncation (…

    Promoter

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAG-EGFP-C-IFT81

    Plasmid

    #218732

    Purpose

    Expresses N-terminally EGFP-tagged IFT81 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Ishida et al Mol Biol Cell. 2022 Aug 1;33(9):ar83. do

    Insert

    IFT81 (IFT81 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAG-EGFP-C-IFT74

    Plasmid

    #218730

    Purpose

    Expresses N-terminally EGFP-tagged IFT74 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Nishijima et al Mol Biol Cell. 2017 Jun 15;28(12):1652-1

    Insert

    IFT74 (IFT74 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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