We narrowed to 4,242 results for: Pet2;

1. pET24a-VHH-1xTS

   Plasmid

   #109418

   Purpose

   Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to one tyrosine sulfation (TS) motif. VHH-1xTS also contains a T7, HA, BAP and His6 epitope

   Depositor

   Martin Spiess

   Article

   Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

   Insert

   anti-GFP nanobody fused to a T7, TS, HA, BAP and His6 epitope
   

   Use

   Tags

   BAP, HA, His6, T7, and Tyrosine sulfation (TS) se…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET22b-ecDHFR-dCys

   Plasmid

   #110541

   Purpose

   Bacterial protein of E.coli DHFR C85A/C152S mutant

   Depositor

   Stephen Benkovic

   Article

   Liu et al J Am Chem Soc. 2014 Jul 23;136(29):10349

   Insert

   ecDHFR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   C85 mutated to A85, C152 mutated to S152

   Promoter

   T7

   Available since

   June 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET27b-ecDHFR-N23PP

   Plasmid

   #110828

   Purpose

   Bacterial expression of E.coli DHFR N23PP mutant

   Depositor

   Stephen Benkovic

   Article

   Bhabha et al Science. 2011 Apr 8;332(6026):234-8. doi

   Insert

   ecDHFR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N23 mutated to P23; a proline residue inserted be…

   Promoter

   T7

   Available since

   June 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28b+R3(R112C/T180C)C

   Plasmid

   #108194

   Purpose

   Expresses PdR-PCNA3(R112C/T180C)-P450cam fusion protein in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

   Insert

   PdR-PCNA3(R112C/T180C)-P450cam fusion protein
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   R112C/T180C(PCNA3)

   Promoter

   T7 promoter

   Available since

   April 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28b+3(R112C/T180C)C

   Plasmid

   #108193

   Purpose

   Expresses PCNA3(R112C/T180C)-P450cam fusion protein in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

   Insert

   PCNA3(R112C/T180C)-P450cam fusion protein
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   R112C/T180C(PCNA3)

   Promoter

   T7 promoter

   Available since

   April 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28-Metavinculin tail

   Plasmid

   #107152

   Purpose

   bacterial expression

   Depositor

   Tina Izard

   Article

   Chinthalapudi et al Proc Natl Acad Sci U S A. 2016 Aug 23;11

   Insert

   metavinculin (VCL Human)
   

   Use

   Tags

   (His)8

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET29b-EPN01(∆M)-His

   Plasmid

   #84510

   Purpose

   E.coli version of EPN01(∆M)

   Depositor

   Neil King

   Article

   Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

   Insert

   EPN01(∆M)-His
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. csd2_121-308/pET21a(+)

   Plasmid

   #83293

   Purpose

   csd2, construct: 121-308, pET21a(+) no-tag

   Depositor

   SeWon Suh

   Article

   An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

   Insert

   hp1544
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   deleted amino acid 1-120

   Promoter

   Available since

   Nov. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. csd5_41-192/pET28b(+)

   Plasmid

   #83296

   Purpose

   csd5, construct: 41-192, pET28b(+) N-tag

   Depositor

   SeWon Suh

   Article

   Kim et al Acta Crystallogr D Biol Crystallogr. 201

   Insert

   hp1250
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   deleted amino acid 1-40

   Promoter

   Available since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. csd4_22-438/pET28b(+)

    Plasmid

    #83295

    Purpose

    csd4, construct: 22-438, pET28b(+) N-tag

    Depositor

    SeWon Suh

    Article

    Kim et al Acta Crystallogr D Biol Crystallogr. 201

    Insert

    hp1075
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    deleted amino acid 1-21

    Promoter

    Available since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

11. csd2_140-251/pET21a(+)

    Plasmid

    #83294

    Purpose

    csd2, construct:140-251, pET21a(+) C-tag

    Depositor

    SeWon Suh

    Article

    An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

    Insert

    hp1544
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-139 and 252-308

    Promoter

    Available since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. csd1_54-312/pET21a(+)

    Plasmid

    #83291

    Purpose

    csd1, construct: 54-312, pET21a(+) C-tag

    Depositor

    SeWon Suh

    Article

    An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

    Insert

    hp1543
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    deleted amino acid 1-53

    Promoter

    Available since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. csd2_121-308/pET28b(+)

    Plasmid

    #83292

    Purpose

    csd2, construct: 121-308, pET28b(+) NC-tag

    Depositor

    SeWon Suh

    Article

    An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

    Insert

    hp1544
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    deleted amino acid 1-120

    Promoter

    Available since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. csd1_125-312/pET28b(+)

    Plasmid

    #83290

    Purpose

    csd1, construct: 125-312, pET28b(+) NC-tag

    Depositor

    SeWon Suh

    Article

    An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

    Insert

    hp1543
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    deleted amino acid 1-124

    Promoter

    Available since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET23a-MmUBB(SnaBI)

    Plasmid

    #69558

    Purpose

    In vitro transcription/translation (T7)

    Depositor

    Jorge Eduardo Azevedo

    Article

    Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

    Insert

    UBB (Ubb Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    The two last codons (TATTAA; encoding the last ty…

    Promoter

    T7

    Available since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pET23a-MmUBB(Y305P)

    Plasmid

    #69557

    Purpose

    Bacterial expression; in vitro transcription/translation (T7)

    Depositor

    Jorge Eduardo Azevedo

    Article

    Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

    Insert

    UBB (Ubb Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon TAT encoding tyrosine 305 was changed to CC…

    Promoter

    T7

    Available since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pET28-p53 (Cow 1-82)

    Plasmid

    #62062

    Purpose

    Expression of Cow p53 (residues 1-82) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Daughdrill et al PLoS One. 2011;6(12):e29207. doi: 10.137

    Insert

    p53
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    contains TAD residues 1-82

    Promoter

    T7

    Available since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pET28-p53 (1-73) (K24N)

    Plasmid

    #62079

    Purpose

    Expression of human p53 (1-73) (K24N) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Zhan et al Proteins. 2013 Oct;81(10):1738-47. doi:

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    contains TAD residues 1-73; K24N

    Promoter

    T7

    Available since

    Jan. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pET28-p53 (Rabbit 1-87)

    Plasmid

    #62058

    Purpose

    Expression of Rabbit p53 (residues 1-87) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Daughdrill et al PLoS One. 2011;6(12):e29207. doi: 10.137

    Insert

    p53
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    contains TAD residues 1-87

    Promoter

    T7

    Available since

    Jan. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pET28A-p53 (1-73) (PPP)

    Plasmid

    #62067

    Purpose

    Expression of human p53 (1-73) (P12A, P13A, P27A) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains TAD residues 1-73; P12A, P13A, P27A

    Promoter

    T7

    Available since

    Jan. 29, 2015

    Availability

    Academic Institutions and Nonprofits only