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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 18,824 results for: nls

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Showing: 1421 - 1440 of 18824 results

  7. BII-STAR-tdT

    Plasmid
    #133396
    Purpose
    Piggybac vector for ASCL2 reporter
    Depositor
    Blair Madison
    Insert
    tdTomato-NLS
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    Stem Cell Specific ASCL2 response elements with L…
    Available since
    Feb. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    133396-plasmid-map-sequence-id-268580

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  14. pCVL.SFFV.Kozak.HA.NLS.SceOpt.IRES.BFP

    Plasmid
    #45574
    Purpose
    Expresses I-Sce I nuclease in mammalian cells with BFP tracker in lentiviral backbone
    Depositor
    Andrew Scharenberg
    Publication
    Kuhar et al Nucleic Acids Res. 2013 Oct 10.
    Insert
    co I-Sce I
    Use
    Lentiviral
    Tags
    HA, IRES BFP, and NLS
    Expression
    Mammalian
    Mutation
    co indicates codon optimization for mammalian exp…
    Promoter
    SFFV
    Available since
    Nov. 4, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    45574-plasmid-map-sequence-id-67191

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  15. pCMVd1-NLS-GAL4-iLight2-VP16-T2A-mTagBFP2

    Plasmid
    #219548
    Purpose
    NLS-Gal4(1-65)-iLight2-VP16-T2A-mTagBFP2 construct expression in mammalian cells.
    Depositor
    Vladislav Verkhusha
    Publication
    Baloban et al Protein Sci. 2024 May;33(5):e4993. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NLS-Gal4(1-65)-iLight2-VP16-T2A-mTagBFP2
    Expression
    Mammalian
    Mutation
    The (SAGG)x4 linker between GAL4(1-65) and the PC…
    Promoter
    CMVd1
    Available since
    May 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    219548-plasmid-map-sequence-id-434071

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  16. pRD406

    Plasmid
    #163111
    Purpose
    Expression of mNeonGreen_H-NSdbd in eukaryotes for visualisation of nuclear DNA
    Depositor
    Remus Dame
    Publication
    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NLS_mNeonGreen_H-NSdbd_NLS
    Use
    Eukaryotic expression
    Expression
    Mammalian
    Promoter
    CMV promoter; T7 promoter
    Available since
    Oct. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    163111-plasmid-map-sequence-id-321832

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  17. pRD398

    Plasmid
    #163105
    Purpose
    Expression of mCherry_H-NSdbd in eukaryotes for visualisation of nuclear DNA
    Depositor
    Remus Dame
    Publication
    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NLS_mCherry_H-NSdbd_NLS
    Use
    Eukaryotic expression
    Expression
    Mammalian
    Promoter
    CMV promoter; T7 promoter
    Available since
    Oct. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    163105-plasmid-map-sequence-id-321846

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  18. pRD399

    Plasmid
    #163106
    Purpose
    Expression of mEGFP_H-NSdbd in eukaryotes for visualisation of nuclear DNA
    Depositor
    Remus Dame
    Publication
    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NLS_mEGFP_H-NSdbd_NLS
    Use
    Eukaryotic expression
    Expression
    Mammalian
    Promoter
    CMV promoter; T7 promoter
    Available since
    Oct. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    163106-plasmid-map-sequence-id-321847

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  19. pX330-Flag-wtSpCas9 (without sgRNA)

    Plasmid
    #92353
    Purpose
    Expression plasmid for human codon-optimized wild-type SpCas9 (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    3xFlag-NLS-wild-type SpCas9-NLS
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Promoter
    Cbh
    Available since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    92353-plasmid-map-sequence-id-178243

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  20. phage - ubc - nls - mcp - egfp - BirA*

    Plasmid
    #131134
    Purpose
    BirA* expressed as a protein fusion with MS2 coat protein (MCP) monomer and EGFP; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.
    Depositor
    Ralf Jansen
    Insert
    NLS-HA-MCP-EGFP-BirA*
    Use
    Lentiviral
    Tags
    NLS, HA
    Expression
    Mammalian
    Mutation
    BirA* = BIrA >R118G
    Promoter
    human ubiquitin C (UBC)
    Available since
    Aug. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    131134-plasmid-map-sequence-id-255499

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Showing: 1421 - 1440 of 18824 results