We narrowed to 9,942 results for: plasmids 101

1. pVH65-Tier1-PTK-Citrine_p2a_SEAP

   Plasmid

   #169559

   Purpose

   Tier-1 vector encoding PTK-driven Citrine-p2A-SEAP expression (PTK-Citrine-p2A-SEAP-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PTK-driven citrine-P2A-SEAP fusion construct
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PTK

   Available since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTS2388-Tier1-PhCMV-p2a_Ypet

   Plasmid

   #169525

   Purpose

   Tier-1 vector encoding PhCMV-driven yellow fluorescent protein YPet fused to a "self-cleaving" peptide p2a (PhCMV-p2a_YPet-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven P2A-fused yellow fluorescent protein Ypet
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTS2387-Tier1-PhCMV-p2a_mTagBFP2

   Plasmid

   #169524

   Purpose

   Tier-1 vector encoding PhCMV-driven blue fluorescent protein mTagBFP2 fused to a "self-cleaving" peptide p2a (PhCMV-p2a_mTagBFP2-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven P2A-fused monomeric Tag blue fluorescent protein 2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTS2389-Tier1-PhCMV-p2a_mRuby

   Plasmid

   #169526

   Purpose

   Tier-1 vector encoding PhCMV-driven red fluorescent protein mRuby2 fused to a "self-cleaving" peptide p2a (PhCMV-p2a_mRuby2-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven P2A-fused enhanced yellow fluorescent protein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTS1024-Tier1-PRPBSA-YPet-p2A-PuroR

   Plasmid

   #169499

   Purpose

   Tier-1 vector encoding PRPBSA-driven YPet in combination with a marker for puromycine selection (PRPBSA-YPet-p2A-PuroR-pA)

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PRPBSA-driven YPet-P2aA-PuroR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PRBSA

   Available since

   June 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTS1047-Tier1-PhCMV-YPet-p2A-PuroR

   Plasmid

   #169498

   Purpose

   Tier-1 vector encoding PhCMV-driven YPet in combination with a marker for puromycin selection (PhCMV-YPet-p2A-PuroR-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven YPet-P2aA-PuroR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available since

   June 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. sg2

   Plasmid

   #113967

   Purpose

   Single short guide RNA targeting TACCACATTTGTAGAGGTT

   Depositor

   Jordan Green

   Article

   Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

   Insert

   sg2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. sg3

   Plasmid

   #113968

   Purpose

   Single short guide RNA targeting CAATGTATCTTATCATGTC

   Depositor

   Jordan Green

   Article

   Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

   Insert

   sg3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. sg2+sg3

   Plasmid

   #113970

   Purpose

   Double short guide RNA targeting TACCACATTTGTAGAGGTT & CAATGTATCTTATCATGTC

   Depositor

   Jordan Green

   Article

   Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

   Insert

   sg2+sg3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pT3EF1aH-myr-Akt

    Plasmid

    #179909

    Purpose

    This plasmid is in the pT3-EF1a vector without loxP sites flanking the inverted repeats of SB (sleeping beauty) sequence. Therefore this plasmid can be used with Cre for in vivo studies.

    Depositor

    Xin Chen

    Article

    Ho et al Hepatology. 2012 Mar;55(3):833-45. doi:

    Insert

    Akt1 (Akt1 Mouse)
    

    Use

    Tags

    HA and Myr

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. DRH001_scAAV-hSyn-iCre-HA

    Plasmid

    #225086

    Purpose

    Expression of iCre with an HA tag in neurons driven by human synapsin promoter.

    Depositor

    Bernardo Sabatini

    Article

    Hochbaum et al Cell. 2024 Oct 3;187(20):5679-5697.e23.

    Insert

    iCre
    

    Use

    AAV and Cre/Lox

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. DRH002_scAAV-hSyn-delta_iCre-HA

    Plasmid

    #225087

    Purpose

    Expression of inactive delta-iCre (control) with an HA tag in neurons driven by human synapsin promoter.

    Depositor

    Bernardo Sabatini

    Article

    Hochbaum et al Cell. 2024 Oct 3;187(20):5679-5697.e23.

    Insert

    delta_iCre
    

    Use

    AAV and Cre/Lox

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p2attB-FST344-HA-CPL

    Plasmid

    #131460

    Purpose

    Encodes human Follistatin protein

    Depositor

    Michele Calos

    Article

    Guha et al Mol Ther Methods Clin Dev. 2019 Oct 14;1

    Insert

    Follistatin (FST Human)
    

    Use

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAS_4xG1 PylT sfGFP 150TAG

    Plasmid

    #154767

    Purpose

    plasmid with 4xG1 PylT cassette and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    150TAG

    Promoter

    EF1

    Available since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-puro/RAF1-S259A

    Plasmid

    #131727

    Purpose

    Mammalian Expression of RAF1

    Depositor

    Jacques De Grève

    Article

    Noeparast et al Oncogene. 2019 Aug;38(31):5933-5941. doi

    Insert

    RAF1 (RAF1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    S259A

    Promoter

    CMV

    Available since

    May 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLenti-puro/RAF1-P261A

    Plasmid

    #131728

    Purpose

    Mammalian Expression of RAF1

    Depositor

    Jacques De Grève

    Article

    Noeparast et al Oncogene. 2019 Aug;38(31):5933-5941. doi

    Insert

    RAF1 (RAF1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    P261A

    Promoter

    CMV

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLenti-puro/RAF1-P207S

    Plasmid

    #131726

    Purpose

    Mammalian Expression of RAF1

    Depositor

    Jacques De Grève

    Article

    Noeparast et al Oncogene. 2019 Aug;38(31):5933-5941. doi

    Insert

    RAF1 (RAF1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    P207S

    Promoter

    CMV

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV:ITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA-ITR

    Plasmid

    #60229

    Purpose

    Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.

    Depositor

    Feng Zhang

    Article

    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

    Inserts

    sgRNA
    Cre recombinase
    

    Use

    AAV, CRISPR, Cre/Lox, and Mouse Targeting

    Tags

    Cre-HA

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and U6

    Available since

    Oct. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. HL 3325 (=HL 770 + rhyB knockout_KanR)

    Bacterial Strain

    #30065

    Depositor

    Han Lim

    Article

    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

    Bacterial Resistance

    Kanamycin

    Species

    Escherichia coli

    Available since

    Aug. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

20. SALM2/LRFN1 scFv [N52B/27]

    Plasmid

    #206747

    Purpose

    Mammalian Expression of SALM2/LRFN1 scFV. Derived from hybridoma N52B/27 scFv.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    SALM2/LRFN1 (Rattus norvegicus) recombinant scFV (Lrfn1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge