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We narrowed to 9,055 results for: sgRNA

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Showing: 1421 - 1440 of 9055 results

  3. lentiCRISPRv2-sgRNAs-del-CDK5RAP2-SVA-BFP

    Plasmid
    #202824
    Purpose
    Lentiviral vector modified to express two sgRNAs that delete the intronic SVA within the CDK5RAP2 gene with EF-1apha for Cas9 and BFP expression
    Depositor
    Matthew Anderson
    Article
    Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:
    Insert
    two sgRNAs (each with a U6 promoter) to delete SVA within CDK5RAP2 gene
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    U6 - two
    Available Since
    July 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    202824-plasmid-map-sequence-id-399641

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  5. lentiCRISPRv2-sgRNAs-del-SCN8A-SVA-BFP

    Plasmid
    #202826
    Purpose
    Lentiviral vector modified to express two sgRNAs that delete the intronic SVA within the SCN8A gene with EF-1apha for Cas9 and BFP expression
    Depositor
    Matthew Anderson
    Article
    Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:
    Insert
    two sgRNAs (each with a U6 promoter) to delete intronic SVA within SCN8A gene
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    U6 - two
    Available Since
    Aug. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    202826-plasmid-map-sequence-id-405885

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  6. lentiCRISPRv2-sgRNAs-del-AK057321-SVA-mcherry

    Plasmid
    #202821
    Purpose
    Lentiviral vector modified to express two sgRNAs that delete the SVA within the SVA-lncRNA AK057321 gene (exon 3) with EF-1apha for Cas9 and mcherry expression
    Depositor
    Matthew Anderson
    Article
    Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:
    Insert
    two sgRNAs (each with a U6 promoter) to delete SVA within SVA-lncRNA AK057321 gene
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    U6 (two)
    Available Since
    July 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    202821-plasmid-map-sequence-id-399746

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  7. lentiCRISPRv2-sgRNAs-del-CHAF1B-SVA-BFP

    Plasmid
    #202825
    Purpose
    Lentiviral vector modified to express two sgRNAs that delete the intronic SVA within the CHAF1B gene with EF-1apha for Cas9 and BFP expression
    Depositor
    Matthew Anderson
    Article
    Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:
    Insert
    two sgRNAs (each with a U6 promoter) to delete intronic SVA within CHAF1B gene
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    U6 - two
    Available Since
    July 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    202825-plasmid-map-sequence-id-399645

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  8. pLKO5.U6.DR130(CasRX)_Non-targeting sgRNA.EFS.tRFP657

    Plasmid
    #212962
    Purpose
    Lentiviral plasmid for the measurement of RfxCas13d (CasRx) nuclease activity. As a marker, the plasmid also encodes the tRFP657 fluorescence protein.
    Depositor
    Roland Rad
    Article
    Montero et al Nat Methods. 2024 Feb 26. doi: 10.1038/s
    Insert
    DR1_CasRx_non-targeting spacer
    Use
    CRISPR and Lentiviral
    Tags
    TagRFP657
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    212962-plasmid-map-sequence-id-422485

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  9. pLKO5.U6.DR130(CasRX)_GFP-targeting sgRNA.EFS.tRFP657

    Plasmid
    #212963
    Purpose
    Lentiviral plasmid for the measurement of RfxCas13d (CasRx) nuclease activity. As a marker, the plasmid also encodes the tRFP657 fluorescence protein.
    Depositor
    Roland Rad
    Article
    Montero et al Nat Methods. 2024 Feb 26. doi: 10.1038/s
    Insert
    DR1_CasRx_GFP targeting spacer
    Use
    CRISPR and Lentiviral
    Tags
    TagRFP657
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    212963-plasmid-map-sequence-id-422499

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  11. pEJS654 All-in-One AAV-sgRNA-hNmeCas9

    Plasmid
    #112139
    Purpose
    Delivery of human-codon-optimized Cas9 from Neisseria meningitidis (NmeCas9) and its single-guide RNA in a single AAV vector for in vivo genome editing.
    Depositor
    Erik Sontheimer
    Article
    Ibraheim et al Genome Biol. 2018 Sep 19;19(1):137. doi:
    Inserts
    sgRNA scaffold
    Human-codon-optimized NmeCas9
    Use
    AAV, CRISPR, and Mouse Targeting
    Expression
    Mammalian
    Promoter
    U1a and U6
    Available Since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    112139-plasmid-map-sequence-id-217823

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  16. U6-sgRNA-BbsI-CMV-EGFP cloning vector

    Plasmid
    #239464
    Purpose
    sgRNA cloning vector with EGFP expression cassette. An sgRNA spacer sequence can be cloned into BbsI sites. A CMV-EGFP expression cassette is included as a reporter of transfection efficiency.
    Depositor
    Patrick Harrison
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6 and CMV
    Available Since
    Dec. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    239464-plasmid-map-sequence-id-476201

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  17. pEJS1084_pTetR-P2A-BFP/BspMI flexible sgRNA

    Plasmid
    #117687
    Purpose
    U6 driven Spy sgRNA cloning vector where guide sequences are inserted between BspMI sites. This plasmid works with Addgene #108570 for subnuclear proteomic profiling via C-BERST method.
    Depositor
    Erik Sontheimer
    Article
    Gao et al Methods Enzymol. 2019;616:365-383. doi:
    Inserts
    Kanamycin cassette
    TetR-P2A-BFP
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6 and hPGK
    Available Since
    Dec. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    117687-plasmid-map-sequence-id-228342

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  19. pX330-Flag-SpCas9-HF1 (without sgRNA)

    Plasmid
    #92102
    Purpose
    Expression plasmid for human codon-optimized high-fidelity SpCas9-HF1, px330-like backbone (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Insert
    3xFlag-NLS-Streptococcus pyogenes Cas9-HF1-NLS
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A
    Promoter
    Cbh
    Available Since
    Oct. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    92102-plasmid-map-sequence-id-197631

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Showing: 1421 - 1440 of 9055 results