We narrowed to 3,209 results for: eed

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ipGL2Cas

Plasmid

#210555

Purpose

Binary vector expressing the estradiol-inducible Lineage Tracing system. XVE expression driven by GL2 promoter.

Depositor

Ortrun Mittelsten Scheid

Article

Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

Insert

GL2p::XVE::UBQ10t::LexA::SpCas9::PEA3AT::VenusSSM

Expression

Plant

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pDGE1176

Plasmid

#202774

Purpose

CRISPR-KO in Arabidopsis thaliana

Depositor

Johannes Stuttmann

Article

Ordon et al Plant Methods. 2023 Mar 28;19(1):30. doi

Inserts

FAST marker
pRPS5a:zCas9_tNbEU
ccdB cassette (BsaI-excisable)

Use

CRISPR

Expression

Plant

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pDGE1177

Plasmid

#202775

Purpose

CRISPR-KO with TRE2 exonuclease co-expression in Arabidopsis thaliana

Depositor

Johannes Stuttmann

Article

Ordon et al Plant Methods. 2023 Mar 28;19(1):30. doi

Inserts

FAST marker
pRPS5a:TREX2-zCas9_tNbEU
ccdB cassette (BsaI-excisable)

Use

CRISPR

Expression

Plant

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pYPQ265K

Plasmid

#173997

Purpose

Gateway entry clone (attL1 & attR5) UNG-rAPOBEC1(R33A)-zCas9(D10A) for C-G base editing

Depositor

Yiping Qi

Article

Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

Insert

UNG-rAPOBEC1(R33A)-zCas9(D10A)

Use

CRISPR

Tags

3X FLAG, NLS

Expression

Plant

Mutation

R33A in rAPOBEC1 and D10A in zCas9

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pKDR-CBh.22

Plasmid

#183566

Purpose

One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated CBh promoter, Fig 3)

Depositor

Erik Dent

Article

Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

Insert

Attenuator Sequence 22

Expression

Mammalian

Promoter

CBh

Available Since

Aug. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pKDR-EF1a.13

Plasmid

#183576

Purpose

One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3)

Depositor

Erik Dent

Article

Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

Insert

Attenuator Sequence 13

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pKDR-TetOn.7

Plasmid

#183581

Purpose

One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated Tet-On promoter, Fig 3)

Depositor

Erik Dent

Article

Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

Insert

Attenuator Sequence 7

Expression

Mammalian

Promoter

Tet-On

Available Since

June 27, 2022

Availability

Academic Institutions and Nonprofits only

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pKDR-TetOn.5

Plasmid

#183580

Purpose

One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated Tet-On promoter, Fig 3)

Depositor

Erik Dent

Article

Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

Insert

Attenuator Sequence 5

Expression

Mammalian

Promoter

Tet-On

Available Since

June 27, 2022

Availability

Academic Institutions and Nonprofits only

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pKDR-TetOn.9

Plasmid

#183582

Purpose

One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated Tet-On promoter, Fig 3)

Depositor

Erik Dent

Article

Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

Insert

Attenuator Sequence 9

Expression

Mammalian

Promoter

Tet-On

Available Since

June 27, 2022

Availability

Academic Institutions and Nonprofits only

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pKDR-TetOn.11

Plasmid

#183583

Purpose

One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated Tet-On promoter, Fig 3)

Depositor

Erik Dent

Article

Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

Insert

Attenuator Sequence 11

Expression

Mammalian

Promoter

Tet-On

Available Since

June 27, 2022

Availability

Academic Institutions and Nonprofits only

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pKDR-CBh.6

Plasmid

#183559

Purpose

One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated CBh promoter, Fig 3)

Depositor

Erik Dent

Article

Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

Insert

Attenuator Sequence 6

Expression

Mammalian

Promoter

CBh

Available Since

June 27, 2022

Availability

Academic Institutions and Nonprofits only

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pKDR-CBh.8

Plasmid

#183560

Purpose

One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated CBh promoter, Fig 3)

Depositor

Erik Dent

Article

Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

Insert

Attenuator Sequence 8

Expression

Mammalian

Promoter

CBh

Available Since

June 27, 2022

Availability

Academic Institutions and Nonprofits only

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pKDR-CBh.13

Plasmid

#183562

Purpose

One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated CBh promoter, Fig 3)

Depositor

Erik Dent

Article

Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

Insert

Attenuator Sequence 13

Expression

Mammalian

Promoter

CBh

Available Since

June 27, 2022

Availability

Academic Institutions and Nonprofits only

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pKDR-CBh.4

Plasmid

#183558

Purpose

One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated CBh promoter, Fig 3)

Depositor

Erik Dent

Article

Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

Insert

Attenuator Sequence 4

Expression

Mammalian

Promoter

CBh

Available Since

June 27, 2022

Availability

Academic Institutions and Nonprofits only

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pKDR-CBh.14

Plasmid

#183563

Purpose

One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated CBh promoter, Fig 3)

Depositor

Erik Dent

Article

Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

Insert

KDR-CBh.14

Expression

Mammalian

Promoter

CBh

Available Since

June 27, 2022

Availability

Academic Institutions and Nonprofits only

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pKDR-CBh.18

Plasmid

#183564

Purpose

One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated CBh promoter, Fig 3)

Depositor

Erik Dent

Article

Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

Insert

Attenuator Sequence 18

Expression

Mammalian

Promoter

CBh

Available Since

June 27, 2022

Availability

Academic Institutions and Nonprofits only

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pKDR-CBh.10

Plasmid

#183561

Purpose

One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated CBh promoter, Fig 3)

Depositor

Erik Dent

Article

Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

Insert

Attenuator Sequence 10

Expression

Mammalian

Promoter

CBh

Available Since

June 27, 2022

Availability

Academic Institutions and Nonprofits only

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shRNA-Ratio-PosCon

Plasmid

#183553

Purpose

Designed to test the efficacy of shRNA by assessing the ratio of GFP-fusion protein to RFP (shRNA ratio positive control (Pbrm1), Figure S2)

Depositor

Erik Dent

Article

Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

Insert

Pbrm1 shRNA

Expression

Mammalian

Promoter

CBh

Available Since

June 27, 2022

Availability

Academic Institutions and Nonprofits only

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shRNA-Ratio-NegCon

Plasmid

#183554

Purpose

Designed to test the efficacy of shRNA by assessing the ratio of GFP-fusion protein to RFP (shRNA ratio negative control (Luciferase), Fig S2)

Depositor

Erik Dent

Article

Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

Insert

Luciferase shRNA

Expression

Mammalian

Promoter

CBh

Available Since

June 27, 2022

Availability

Academic Institutions and Nonprofits only

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pKDR-TetOn.13

Plasmid

#183584

Purpose

One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated Tet-On promoter, Fig 3)

Depositor

Erik Dent

Article

Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

Insert

Attenuator Sequence 13

Expression

Mammalian

Promoter

Tet-On

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pKDR-EF1a.10

Plasmid

#183573

Purpose

One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3)

Depositor

Erik Dent

Article

Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

Insert

Attenuator Sequence 10

Expression

Mammalian

Promoter

EF1a

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pKDR-EF1a.11

Plasmid

#183574

Purpose

One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3)

Depositor

Erik Dent

Article

Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

Insert

Attenuator Sequence 11

Expression

Mammalian

Promoter

EF1a

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pKDR-EF1a.12

Plasmid

#183575

Purpose

One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3)

Depositor

Erik Dent

Article

Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

Insert

Attenuator Sequence 12

Expression

Mammalian

Promoter

EF1a

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pKDR-EF1a.5

Plasmid

#183572

Purpose

One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3)

Depositor

Erik Dent

Article

Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

Insert

Attenuator Sequence 5

Expression

Mammalian

Promoter

EF1a

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pKDR-EF1a.3

Plasmid

#183570

Purpose

One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3)

Depositor

Erik Dent

Article

Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

Insert

Attenuator Sequence 3

Expression

Mammalian

Promoter

EF1a

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pKDR-EF1a.1

Plasmid

#183568

Purpose

One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3)

Depositor

Erik Dent

Article

Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

Insert

Attenuator Sequence 1

Expression

Mammalian

Promoter

EF1a

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pKDR-CBh.21

Plasmid

#183565

Purpose

One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated CBh promoter, Fig 3)

Depositor

Erik Dent

Article

Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

Insert

Attenuator Sequence 21

Expression

Mammalian

Promoter

CBh

Available Since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMRBad-C-DiB-RM

Plasmid

#168478

Purpose

C-fragment of the DiB-RM‐split protein. This plasmid needs to be co-transformed with Plasmid 168476: pET11a-N-DiB-RM to obtain DiB-RM‐split protein

Depositor

Jens Meiler

Article

Bozhanova et al PLoS Comput Biol. 2021 Nov 8;17(11):e100

Insert

C-fragment of DiB-RM

Expression

Bacterial

Promoter

araBAD

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pET11a-N-DiB-RM

Plasmid

#168476

Purpose

N-fragment of the DiB-RM‐split protein. This plasmid needs to be co-transformed with Plasmid 168478: pMRBad-C-DiB-RM to obtain DiB-RM‐split protein

Depositor

Jens Meiler

Article

Bozhanova et al PLoS Comput Biol. 2021 Nov 8;17(11):e100

Insert

N-fragment of DiB-RM

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pET11a-Z-N-DiB2

Plasmid

#168473

Purpose

N-fragment of the DiB2‐split‐Zip protein. This plasmid needs to be co-transformed with Plasmid 168256: pMRBad-Z-C-wtBlc to obtain DiB2‐split-Zip protein

Depositor

Jens Meiler

Article

Bozhanova et al Sci Rep. 2020 Jul 6;10(1):11049. doi: 10

Insert

N-fragment of DiB2 + Leucine Zipper

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pET11a-Z-N-wtBlc

Plasmid

#168258

Purpose

N-fragment of the wtBlc‐split‐Zip protein. This plasmid needs to be co-transformed with Plasmid 168256: pMRBad-Z-C-wtBlc to obtain wtBlc‐split-Zip protein

Depositor

Jens Meiler

Article

Bozhanova et al FEBS Lett. 2021 Jan;595(2):206-219. doi:

Insert

N-fragment of wtBlc + Leucine Zipper

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
plIVMD163

Plasmid

#160935

Purpose

In vivo mRNA display construct for mCherry

Depositor

Saeed Tavazoie

Article

Oikonomou et al Proc Natl Acad Sci U S A. 2020 Oct 9. pi

Insert

MCP-mCherry-HIS tag, 3'UTR MS2 Stem Loop

Tags

HIS tag, 3'UTR MS2 Stem Loop

Expression

Yeast

Promoter

MET25

Available Since

Nov. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBXMCS-2 Pconstitutive-sgRNA(Spa)_gcrA3

Plasmid

#133343

Purpose

for constitutive expression of a single guide RNA from Streptococcus pasteurianus with a seed region that targets gcrA gene's promoter from Caulobacter crescentus

Depositor

Michael Laub

Article

Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

Insert

sgRNA_gcrA

Use

CRISPR

Promoter

constitutive

Available Since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBXMCS-2 Pconstitutive-sgRNA(Sth3)_blaA

Plasmid

#133345

Purpose

for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets blaA gene's promoter from Caulobacter crescentus

Depositor

Michael Laub

Article

Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

Insert

sgRNA_blaA

Use

CRISPR

Promoter

constitutive

Available Since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
MTK3a_023

Plasmid

#123775

Purpose

Encodes Absisic acid insensitive 1 taggeed with his and tagBFP as a Type 3a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

his::ABI::tagBFP::linker

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTCW015

Plasmid

#83530

Purpose

UBiC and feedback resistant ARO4 expression from pheromone inducible FUS1J2 promoter

Depositor

Claudia Vickers

Article

Williams et al Metab Eng. 2015 May;29:124-134. doi: 10.

Insert

pFUS1J2-UBiC-CYC1t-pFUS1J2-ARO4*-CYC1t

Expression

Yeast

Promoter

pFUS1J2

Available Since

Dec. 16, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pBXMCS-2 Pconstitutive-sgRNA(Sth3)_gcrA2

Plasmid

#133341

Purpose

for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets gcrA gene's promoter on the template strand in Caulobacter crescentus

Depositor

Michael Laub

Article

Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

Insert

sgRNA_gcrA

Use

CRISPR

Promoter

constitutive

Availability

Academic Institutions and Nonprofits only

Enlarge
BPK1520

Plasmid

#65777

Purpose

Human expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNA

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

SpCas9 gRNA backbone, without spacer sequence

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
BPK3079

Plasmid

#78741

Purpose

Human expression plasmid for AsCpf1 guide RNA (need to clone in spacer into BsmBI sites)

Depositor

Keith Joung

Article

Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

Insert

AsCpf1 crRNA backbone, without spacer sequence

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 16, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
ampl43

Plasmid

#161830

Purpose

pRS416-dCas9-Mxi1+TetR+pRPR1(TetO)-NotI-gRNA plasmid marked with His, expressing dCas9-Mxi1 under Tef1 promoter, and a tet-inducible promoter for gRNA expression.

Depositor

Saeed Tavazoie

Article

Momen-Roknabadi et al Commun Biol. 2020 Nov 27;3(1):723. doi:

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Promoter

TEF1

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge

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