Addgene

We narrowed to 4,764 results for: IRES

Showing: 1441 - 1460 of 4764 results

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pHAGE-EF1a MICA*009-IRES-ZsGreen

Plasmid

#114007

Purpose

Induces expression of human MICA allele 009 cDNA

Depositor

Kai Wucherpfennig

Article

Ferrari de Andrade et al Science. 2018 Mar 30;359(6383):1537-1542

Insert

MICA*009 (MICA Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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MSCV-human-c-MYC-ER-mouse IRES-mcherry

Plasmid

#73570

Purpose

Mammalian retroviral expression of Myc-ER

Depositor

Martine Roussel

Article

Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

Insert

cMYC (MYC Human)

Use

Retroviral

Tags

IRES-mCherry and Mouse ER

Expression

Mammalian

Mutation

Promoter

MSCV

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only
pLVX-EF1a-EGFP-VAMP2-IRES-Puromycin

Plasmid

#134661

Purpose

Expresses EGFP-tagged synaptic vesicle protein

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

vesicle-associated membrane protein 2 (VAMP2 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-IRES-3xNLS-mScarlet-I3-pA (JDW 1360)

Plasmid

#224536

Purpose

A Gateway compatible 3' entry clone containing IRES-FLAG- NLS-mScarlet-I3

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

IRES-FLAG- NLS-mScarlet-I3

Use

Gateway cloning

Tags

Expression

Mutation

Promoter

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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MSCV-EZH2-PGK-Puro-IRES-GFP

Plasmid

#75125

Purpose

Retroviral expression plasmid encoding wild type human EZH2

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

KMT6 (EZH2 Human)

Use

Retroviral

Tags

None

Expression

Mutation

Promoter

Available since

May 20, 2016

Availability

Academic Institutions and Nonprofits only
pDisplay-GRAB-rDA2m-IRES-EGFP-CAAX

Plasmid

#208694

Purpose

Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA2m and a membrane-localized EGFP in mammalian cells

Depositor

Yulong Li

Article

Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

GPCR activation based dopamine (DA) sensor GRAB_rDA2m

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-Flag-CDK8-W105M-IRES-mCherry

Plasmid

#68857

Purpose

Expresses Human CDK8 W105M Mutant

Depositor

Matthew Shair

Article

Pelish et al Nature. 2015 Sep 28. doi: 10.1038/nature

Insert

Cyclin-Dependent Kinase 8 (CDK8 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

W105M

Promoter

EF1α

Available since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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CAG-DDdCas9VP192-T2A-EGFP-ires-puro

Plasmid

#69534

Purpose

DHFR destabilised domain (DD) fused to dCas9VP192 (S.pyogenes) on CAG expression vector. DDdCas9VP192 protein is stabilised by Trimethoprim.

Depositor

Timo Otonkoski

Article

Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

Insert

DD-dCas9VP192-T2A-EGFP

Use

Tags

C-term T2A-EGFP and DHFR Destabilised Domain

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only

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SARS-CoV-2 Nucleocapsid-3xHA-IRES-mcherry

Plasmid

#173030

Purpose

To express SARS-CoV-2 Nucleocapsid protein tagged with 3xHA and in frame with mcherry (NCP-IRES-mcherry) in mammalian cells

Depositor

Mohamed Fareh

Article

Fareh et al Nat Commun. 2021 Jul 13;12(1):4270. doi:

Insert

SARS-CoV-2 Nucleocapsid phosphoprotein

Use

Lentiviral

Tags

3xHA and IRES-mcherry

Expression

Mammalian

Mutation

Promoter

Gag (truncated)

Available since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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AAV SYN PSAM4 GlyR IRES EGFP

Plasmid

#119742

Purpose

Chemogenetic inhibitor expression plasmid

Depositor

Scott Sternson

Article

Magnus et al Science. 2019 Mar 14. pii: science.aav52

Has Service

AAV5

Insert

PSAM4 GlyR IRES eGFP (CHRNA7 Human, Synthetic)

Use

AAV

Tags

Expression

Mammalian

Mutation

L131G, Q139L, Y217F

Promoter

synapsin

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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AiP935-Batf3-IRES2-FlpO-WPRE-neo

Plasmid

#181771

Purpose

targeting vector

Depositor

Tanya Daigle

Insert

Batf3 homology arms, FlpO

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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MSCV-human CSF-1Rwt-IRES-GFP

Plasmid

#115523

Purpose

Mammalian expression construct

Depositor

Martine Roussel

Article

Roussel et al Nature. 1987 Feb 5-11;325(6104):549-52.

Insert

human CSF-1 receptor (CSF1R Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP/CALR del52-FLAG

Plasmid

#204523

Purpose

Retroviral expression of human CALR del52-FLAG

Depositor

Norio Komatsu

Article

Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

Insert

CALR del52-FLAG (CALR Human)

Use

Retroviral

Tags

Expression

Mutation

del52

Promoter

Available since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pDisplay-GRAB-rDA2h-IRES-EGFP-CAAX

Plasmid

#208695

Purpose

Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA2h and a membrane-localized EGFP in mammalian cells

Depositor

Yulong Li

Article

Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

GPCR activation based dopamine (DA) sensor GRAB_rDA2h

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-CAG-NOTCH2NLB (N2NL-FL)-ires-mCherry

Plasmid

#122957

Purpose

Lentiviral expression of NOTCH2NLB full length under CAG promoter with mCherry expression

Depositor

Pierre Vanderhaeghen

Article

Suzuki et al Cell. 2018 May 31;173(6):1370-1384.e16.

Insert

NOTCH2NLB (NOTCH2NLB Human)

Use

Lentiviral

Tags

6xmyc tag

Expression

Mutation

Promoter

CAG promoter

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX-hSyn-Galpha-i3-IRESWT-Gbetagamma ONE-GO

Plasmid

#204343

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

hSyn Galpha-i3/Gbetagamma ONE-GO

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP/CALR ins5-FLAG

Plasmid

#204524

Purpose

Retroviral expression of human CALR ins5-FLAG

Depositor

Norio Komatsu

Article

Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

Insert

CALR ins5-FLAG (CALR Human)

Use

Retroviral

Tags

Expression

Mutation

ins5

Promoter

Available since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-HA-rM3D(Gs)-IRES-mCitrine

Plasmid

#50456

Purpose

Double floxed Gs-coupled rM3D-IRES-mCitrine under the control of human synapsin promoter

Depositor

Bryan Roth

Insert

rM3D-IRES-mCitrine (CHRM3 Human)

Use

AAV

Tags

HA

Expression

Mutation

See supplemental documents for DREADD mutations

Promoter

human Synapsin 1

Available since

April 16, 2014

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-MinE(L4E)-EGFP_IRES-Blast

Plasmid

#213117

Purpose

Expression of a faster-running MinE mutant in mammalian cells

Depositor

Scott Coyle

Article

Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

Insert

MinE(L4E)-EGFP (minE Escherichia coli)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

L4E

Promoter

EF1a

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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H516 SONIC KrasG12D-IRES-luciferase donor

Plasmid

#138178

Purpose

CRISPR SONIC: Kras G12D luciferase donor plasmid.

Depositor

Wen Xue

Article

Mou et al Genome Med. 2019 Apr 16;11(1):21. doi: 1

Insert

KrasG12D (Kras Mouse)

Use

Nhej donor

Tags

Expression

Mutation

Promoter

Available since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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