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pYPQ131-STU-Fn

Plasmid

#138095

Purpose

Golden Gate entry vector for 1st crRNA cloning with FnCas12a crRNA scaffold flanked by HH and HDV ribozymes

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ132-STU-Fn

Plasmid

#138098

Purpose

Golden Gate entry vector for 2nd crRNA cloning with FnCas12a crRNA scaffold flanked by HH and HDV ribozymes

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ133-STU-Fn

Plasmid

#138101

Purpose

Golden Gate entry vector for 3rd crRNA cloning with FnCas12a crRNA scaffold flanked by HH and HDV ribozymes

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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AAV_Dbh HMEJ donor

Plasmid

#97320

Purpose

HMEJ donor for fusing a p2A-mCherry-WPRE reporter to mouse Dbh. AAV backbone.

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Dbh HMEJ donor

Use

AAV and Mouse Targeting

Expression

Mammalian

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pYPQ132-STU-As

Plasmid

#138097

Purpose

Golden Gate entry vector for 2nd crRNA cloning with AsCas12a crRNA scaffold flanked by HH and HDV ribozymes

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ133-STU-As

Plasmid

#138100

Purpose

Golden Gate entry vector for 3rd crRNA cloning with AsCas12a crRNA scaffold flanked by HH and HDV ribozymes

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pmCherry-TEAD4-YBD

Plasmid

#166450

Purpose

Expresses fusion of mCherry and TEAD4-YAP binding domain

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mCherry, TEAD4-YBD(217-434) (TEAD4, YAP1 Human)

Expression

Mammalian

Available Since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti-Cry2-mCherry- YAP-C-IDR

Plasmid

#166447

Purpose

Expresses fusion of CRY2PHR with mCherry and YAP-C-IDR

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

Cry2, mCherry, YAP-C-IDR(266-504) (YAP1, CRY2 Human)

Use

Lentiviral

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti-Cry2-mCherry-TEAD4-IDR

Plasmid

#166449

Purpose

Expresses fusion of CRY2PHR with mCherry and TEAD4-IDR

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

Cry2, mCherry, TEAD4-IDR(89-230) (TEAD4, CRY2 Human)

Use

Lentiviral

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti-Cry2-mCherry- YAP-N-IDR

Plasmid

#166446

Purpose

Expresses fusion of CRY2PHR with mCherry and YAP-N-IDR

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

Cry2, mCherry, YAP-N-IDR(1-164) (YAP1, CRY2 Human)

Use

Lentiviral

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti-117G-hyeA3A-BE4max

Plasmid

#157946

Purpose

Lentiviral expression of hyeA3A-BE4max

Depositor

Dali Li

Article

Zhang et al Nat Cell Biol. 2020 Jun;22(6):740-750. d

Insert

Lenti -117G-hyeA3A-BE4max

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

N57G

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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Lenti-117G-hyA3A-BE4max

Plasmid

#157945

Purpose

Lentiviral expression of hyA3A-BE4max

Depositor

Dali Li

Article

Zhang et al Nat Cell Biol. 2020 Jun;22(6):740-750. d

Insert

Lenti-117G- hyA3A-BE4max

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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LentiU6-LacZ-GFP-Puro (BB)

Plasmid

#170459

Purpose

For cloning gRNA block

Depositor

Yonglun Luo

Article

Xiang et al Nat Commun. 2021 May 28;12(1):3238. doi:

Insert

hU6-BB-LacZ and EF1A-EGFP-2A-Puro

Use

Lentiviral

Expression

Mammalian

Promoter

hU6 for gRNA and EF1A for EGFP-2A-Puro

Available Since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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AAV_Actb HMEJ donor_U6_sgRNA_EF1a_GFP_polyA

Plasmid

#97308

Purpose

HMEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Actb HMEJ donor

Use

AAV and Mouse Targeting

Expression

Mammalian

Promoter

U6, EF1a

Available Since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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AAV_Actb MMEJ donor_U6_sgRNA_EF1a_GFP_polyA

Plasmid

#97311

Purpose

MMEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Actb MMEJ donor

Use

AAV and Mouse Targeting

Expression

Mammalian

Promoter

U6, EF1a

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pYPQ289 (ErCas12a)

Plasmid

#138126

Purpose

Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized ErCas12a without promoter

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Insert

ErCas12a

Use

CRISPR; Gateway compatible cas12a entry clone

Tags

NLS

Expression

Plant

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti_Actb HMEJ donor_U6_sgRNA_EF1a_GFP_polyA

Plasmid

#97312

Purpose

HMEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. Lenti backbone.

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Actb HMEJ donor

Use

Lentiviral and Mouse Targeting

Expression

Mammalian

Promoter

U6, EF1a

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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AAV_Actb HR donor_U6_sgRNA_EF1a_GFP_polyA

Plasmid

#97309

Purpose

HR donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Actb HR donor

Use

AAV and Mouse Targeting

Expression

Mammalian

Promoter

U6, EF1a

Available Since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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AAV_Actb NHEJ donor_U6_sgRNA_EF1a_GFP_polyA

Plasmid

#97310

Purpose

NHEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Actb NHEJ donor

Use

AAV and Mouse Targeting

Expression

Mammalian

Promoter

U6, EF1a

Available Since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pYPQ282 (TsCas12a)

Plasmid

#138114

Purpose

Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized TsCas12a without promoter

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Insert

TsCas12a

Use

CRISPR; Gateway compatible cas12a entry clone

Tags

NLS

Expression

Plant

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge