We narrowed to 23,252 results for: Sele
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Plasmid#145095PurposeExpressing base editor cCDA1-NGBE3 in yeast cellsDepositorInsertcCDA1-NGBE3
UseCRISPRExpressionYeastMutationspCas9(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1…PromoterGalLAvailable SinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
HOXB3 (human) HIS-tag pET
Plasmid#8524DepositorInsertHOXB3 (HOXB3 Human)
TagsHis and T7ExpressionBacterialMutationContains 5 additional amino acids before ATG from…Available SinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-HLA-A3-VN
Plasmid#135506PurposeMammalian expression of VN-fused and myc-tagged HLA-A*03:01DepositorInsertHLA-A*03:01 (HLA-A Human)
TagsExtracellular c-myc epitope tag, Signal peptide f…ExpressionMammalianPromoterCMVAvailable SinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-161K enhancer-ETS-CORE-MUT-minP:fLuc-TK:rLuc
Plasmid#138367PurposeLentiviral plasmid contains 161k modified human enhancer with with ETS binding sites mutated driving firefly Luciferase 2 and Renilla Luciferase driven by TK promoterDepositorInsert161K human enhancer, with ETS binding sites mutated, driving firefly enhancer and TK driving refills luciferase
UseLentiviralExpressionMammalianAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLG1-puro-sgATL2-1
Plasmid#109008PurposeCRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)DepositorAvailable SinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag MKK3b(Glu, delete LXL)
Plasmid#50450Purposeactivates p38 in the signaling pathwayDepositorInsertMKK3b (MAP2K3 Human)
TagsFlagExpressionMammalianMutationdeleted L25-I27, changed Serine 218 to Glutamic A…PromoterCMVAvailable SinceJan. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLenti-161K enhancer-NR2F2-MUT-minP:fLuc-TK:rLuc
Plasmid#138368PurposeLentiviral plasmid contains 161k modified human enhancer with with NR2F2 binding sites mutated driving firefly Luciferase 2 and Renilla Luciferase driven by TK promoterDepositorInsert161K human enhancer, with NR2F2 binding sites mutated, driving firefly enhancer and TK driving renilla luciferase
UseLentiviralExpressionMammalianAvailable SinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ134-STU-Fn
Plasmid#138104PurposeGolden Gate entry vector for 4th crRNA cloning with FnCas12a crRNA scaffold flanked by HH and HDV ribozymesDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable SinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ131-STU-Fn
Plasmid#138095PurposeGolden Gate entry vector for 1st crRNA cloning with FnCas12a crRNA scaffold flanked by HH and HDV ribozymesDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable SinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ132-STU-Fn
Plasmid#138098PurposeGolden Gate entry vector for 2nd crRNA cloning with FnCas12a crRNA scaffold flanked by HH and HDV ribozymesDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable SinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ133-STU-Fn
Plasmid#138101PurposeGolden Gate entry vector for 3rd crRNA cloning with FnCas12a crRNA scaffold flanked by HH and HDV ribozymesDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable SinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-HLA-A24-VN
Plasmid#135508PurposeMammalian expression of VN-fused and myc-tagged HLA-A*24:02DepositorInsertHLA-A*24:02 (HLA-A Human)
TagsExtracellular c-myc epitope tag, Signal peptide f…ExpressionMammalianPromoterCMVAvailable SinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
HOXD4 (murine) HIS-tag pET
Plasmid#8548DepositorInsertHOXD4 (Hoxd4 Mouse)
TagsHis and T7ExpressionBacterialMutationmakes protein by Western and by in vitro transcri…Available SinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pEBTetBl-CLIP-Cep57
Plasmid#136866PurposeMammalian expression of the centrosomal protein Cep57 N-terminally fused to CLIP-tagDepositorInsertCLIP-Cep57 (CEP57 Synthetic, Human)
TagsFLAG-tag / His-tagExpressionMammalianPromoterCMV-TetO2Available SinceMarch 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLG1-puro-sgATL1-5
Plasmid#109007PurposeCRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)DepositorAvailable SinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-HLA-A1-VN
Plasmid#135502PurposeMammalian expression of VN-fused and myc-tagged HLA-A*01:01DepositorInsertHLA-A*01:01 (HLA-A Human)
TagsExtracellular c-myc epitope tag, Signal peptide f…ExpressionMammalianPromoterCMVAvailable SinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBTetD-SNAP-CP110
Plasmid#136803PurposeMammalian expression of the centrosomal protein Cep110 N-terminally fused to SNAP-tagDepositorInsertSNAP-CP110 (CCP110 Synthetic, Human)
TagsFLAG-tag / His-tagExpressionMammalianPromoterCMV-TetO2Available SinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pbIG-Xph20-ETWV
Plasmid#133034PurposeExpresses Xph20-ETWV in bacteriaDepositorInsertXph20-ETWV
TagsAviTag, His10, and TEV protease cleavage siteExpressionBacterialMutationS63KPromoterT7Available SinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC39A5
Plasmid#132057PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC39A5 (SLC39A5 Human)
ExpressionMammalianAvailable SinceNov. 8, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGSTag Flag p38 alpha (agf)
Plasmid#20816DepositorInsertpGSTag Flag p38 alpha (agf) (Mapk14 Mouse)
TagsFlagExpressionBacterialMutationReplace Thr 180 and Tyr 182 with Ala and Phe resp…Available SinceApril 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLG1-puro-sgATL2-2
Plasmid#109009PurposeCRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)DepositorAvailable SinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-C21orf7
Plasmid#34810DepositorAvailable SinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBBR1-EcTyrRS(VSMA,D165G)
Plasmid#231132PurposeExpresses mutant E. coli TyrRS from a derepressed lac promoter for proteome-wide incorporation of tyrosine in E. coli/K. pneumoniae.DepositorInsertE. coli tyrosyl tRNA synthetase
UseKlebsiella pneumoniae expressionExpressionBacterialMutationY37V, D165G, D182S, P183M, L186A, D265RAvailable SinceOct. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
sgRNA-Dual_filler(hU6_H1)-EF1a-Thy1.1-P2A-Neo
Plasmid#239608PurposeBackbone for the cloning of a dual sgRNA expression plasmid (hU6 and H1 promoters) using BsmbI. Selectable with a G418 resistanceDepositorInsertfiller-trcr-H1-filler
UseCRISPR and LentiviralAvailable SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(hMTOR_1k)-PGKpuro2ABFP-W
Plasmid#208429PurposeLentiviral gRNA plasmid targeting human MTOR gene, co-expression of BFP tagDepositorInsertMTOR (MTOR Human)
UseCRISPR and LentiviralExpressionMammalianMutationN/APromoterhuman U6Available SinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(hMTOR_2b)-PGKpuro2ABFP-W
Plasmid#208430PurposeLentiviral gRNA plasmid targeting human MTOR gene, co-expression of BFP tagDepositorInsertMTOR (MTOR Human)
UseCRISPR and LentiviralExpressionMammalianMutationN/APromoterhuman U6Available SinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUHD 10-3-human CDK11 p110B wt.-FLAG
Plasmid#240138PurposeExpress CDK11 p110B geneDepositorAvailable SinceJune 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-FLAG-Rgs14
Plasmid#236396PurposeExpresses FLAG-Rgs14 in mammalian cellsDepositorInsertRattus norvegicus regulator of G-protein signaling 14 (Rgs14 Rat)
TagsFLAGExpressionMammalianAvailable SinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEIF3D-WT-sgNT
Plasmid#222120PurposeEIF3D overexpression vector with non-targeting sgRNADepositorAvailable SinceMarch 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJExpress401-KDAC8 D101N
Plasmid#224348PurposeExpresses human KDAC8 (HDAC8) D101N in E. coliDepositorInsertKDAC8 (HDAC8 Human)
TagsTEV-cleavable His6ExpressionBacterialMutationAspartate 101 mutated to asparaginePromoterT5Available SinceFeb. 19, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.1 T21R-Nb139-T2A-mCherry
Plasmid#225589PurposeMammalian expression vector for TRIM21 RING-Nb139 anti-p53 degrader with a self-cleaving T2A-mCherry fluorescent expression reporterDepositorInsertT21R-Nb139-T2A-mCherry
TagsmCherryExpressionMammalianPromoterCMVAvailable SinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4TO T21R-T2A-mCherry
Plasmid#225585PurposeMammalian expression vector for Doxycycline inducible expression of TRIM21 RING with a self-cleaving T2A-mCherry fluorescent expression reporterDepositorInsertT21R-T2A-mCherry
TagsmCherryExpressionMammalianPromoterCMVAvailable SinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEIF3D-RNAgateDel-sgEIF3D
Plasmid#222137PurposeEIF3D variant overexpression vector with sgRNA targeting eIF3DDepositorAvailable SinceFeb. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEIF3D-NtermDel-sgEIF3D
Plasmid#222123PurposeEIF3D variant overexpression vector with sgRNA targeting eIF3DDepositorAvailable SinceFeb. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CMV-Puro-P2A-NES/PKI-mNeonGreen2x-NLS/FUS
Plasmid#228400PurposeEncodes NES/PKI-mNeonGreen2x-NLS/FUS which is a tandem of mNeongreen with an N-terminal PKI NES and a C-terminal FUS NLS that shuttles between the nucleus and the cytoplasmDepositorInsertNES/PKI-mNeonGreen2x-NLS/FUS
ExpressionMammalianPromoterCMVAvailable SinceJan. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGL4-CMV-NLS/cMyc-AcGFP2x-NES/iKB2-P2A-Puro
Plasmid#228398PurposeEncodes NLS/cMyc-AcGFP2x-NES/iKB2 which is a tandem of AcGFP with an N-terminal cMyc NLS and a C-terminal iKB2 NES that shuttles between the nucleus and the cytoplasmDepositorInsertNLS/cMyc-AcGFP2x-NES/iKB2
ExpressionMammalianPromoterCMVAvailable SinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGL4-CMV-3xFLAG-NLS/SV40-AcGFP-NES/PKI-P2A-Puro
Plasmid#228399PurposeEncodes 3xFLAG-NLS/SV40-AcGFP-NES/PKI which is a AcGFP with an N-terminal SV40 NLS and a C-terminal PKI NES that shuttles between the nucleus and the cytoplasmDepositorInsert3xFLAG-NLS/SV40-AcGFP-NES/PKI
ExpressionMammalianPromoterCMVAvailable SinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUt-mNF-Cas13d
Plasmid#224789PurposeLPUtopia matching RMCE donor plasmid with mCherry-P2A-RfxCas13d Negative-Feedback circuit. Use BlastR for positive selection and HSV-TK for negative selection.DepositorInsertmCherry-P2A-RfxCas13d
UseCRISPR and Synthetic BiologyExpressionMammalianPromoterCMV-d2iAvailable SinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJExpress401-KDAC8 D101R
Plasmid#224349PurposeExpresses human KDAC8 (HDAC8) D101R in E. coliDepositorInsertKDAC8 (HDAC8 Human)
TagsTEV-cleavable His6ExpressionBacterialMutationAspartate 101 mutated to argininePromoterT5Available SinceOct. 28, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJExpress401-KDAC8 D101A
Plasmid#224347PurposeExpresses human KDAC8 (HDAC8) D101A in E. coliDepositorInsertKDAC8 (HDAC8 Human)
TagsTEV-cleavable His6ExpressionBacterialMutationAspartate 101 mutated to alaninePromoterT5Available SinceOct. 22, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits