Skip to main content
Addgene
Showing: 1461 - 1480 of 17137 results

  1. pSF4 TetCMV ATF4(5'UTR)-SunTag-Renilla-FKBP-Stop-24xMS2v5-CTE-polyA

    Plasmid
    #164615
    Purpose
    Expresses ATF4(5'UTR)-SunTag-Renilla mRNA reporter under tetracycline-inducible promoter. SunTag and Renilla luciferase are in frame with the main start codon of ATF4.
    Depositor
    Jeffrey Chao
    Article
    Mateju et al Cell. 2020 Dec 23;183(7):1801-1812.e13.
    Insert
    Renilla luciferase
    Use
    Tags
    24xMS2v5 (3'UTR) and SunTag
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    TetCMV
    Available since
    Sept. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    164615-plasmid-map-sequence-id-325006

    Enlarge

  2. pSF4 TetCMV intron 20xGCN4 Renilla Rh1 STOP 24xMS2v5 SV40 CTE polyA

    Plasmid
    #105000
    Purpose
    Expresses ST-Renilla-Rh1 calibration reporter in mammalian cells; note plasmid contains 24xGCN4 repeats
    Depositor
    Jeffrey Chao
    Article
    Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.
    Insert
    Renilla luciferase
    Use
    Tags
    24xGCN4 repeats (SunTag), 24xMS2v5 stem loops in …
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    TetCMV
    Available since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105000-plasmid-map-sequence-id-203163

    Enlarge

  4. pSF4 TetCMV 5'TOP intron 20xGCN4 Renilla FKBP Stop 24xMS2v5 SV40 CTE polyA

    Plasmid
    #119946
    Purpose
    Expresses 5'TOP-SunTag-Renilla mRNA reporter in mammalian cells; note plasmid contains 24xGCN4 repeats
    Depositor
    Jeffrey Chao
    Article
    Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18
    Insert
    Renilla luciferase
    Use
    Tags
    24xGCN4 repeats (SunTag), 24xMS2v5 stem loops in …
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    TetCMV
    Available since
    March 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    119946-plasmid-map-sequence-id-237566

    Enlarge

  7. pB6-Hipp11-H2B-sfGFP-T2A-tdTomato-cre reporter-3pA stop-FNF-DTA

    Plasmid
    #183027
    Purpose
    B6J Hipp11-FNF-pCAG-loxP-3xSV40pA-loxP-H2B-sfGFP-T2A-tdTomato-WPRE-bpA allele targeting vector, low copy number
    Depositor
    Mario Capecchi
    Insert
    H2B-sfGFP-T2A-tdTomato
    Use
    Cre/Lox and Mouse Targeting
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    April 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    183027-plasmid-map-sequence-id-360504

    Enlarge

  11. pNW538_pAAVS1-pAP1(2)-FlpO-ABI-P2A-PYL-FlpO-CAG-U1-frt-STOP-frt-U2-GFP-P2A-luc2-ZeoR

    Plasmid
    #192939
    Purpose
    FlpO-based digitizer circuit driven by AP1 promoter
    Depositor
    Wilson Wong
    Article
    Wong et al Nat Commun. 2021 Oct 22;12(1):6150. doi:
    Inserts
    AP1(2)/minTK promoter
    FlpO-ABI
    PYL-FlpO
    CAG promoter
    frt-STOP-frt
    GFP
    luciferase
    pPGK
    Zeocin resistance
    BFP
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    192939-plasmid-map-sequence-id-380399

    Enlarge

  13. Ralstonia-GG-Kit

    Plasmid Kit
    #1000000142
    Purpose
    Backbone vectors and Level I modules that can be used for assembly of Ralstonia solanacearum species complex gene knockout and complementation constructs based on Golden Gate cloning techniques
    Depositor
    Thomas Lahaye
    Available since
    Dec. 18, 2018
    Availability
    Academic Institutions and Nonprofits only

  14. PRESTO-Tango Kit

    Plasmid Kit
    #1000000068
    Purpose
    Used to measure receptor activation for more than 300 G-protein coupled receptors (GPCRs) via a modified Tango beta-arrestin recruitment assay.
    Depositor
    Bryan Roth
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only

  15. Myristoylated Kinase Library

    Plasmid Kit
    #1000000012
    Purpose
    190 human kinases and kinase-related open reading frames (ORFs) cloned into a retroviral vector which adds a myristoylation sequence and flag-epitope tag to each ORF.
    Depositor
    William Hahn
    Available since
    July 16, 2011
    Availability
    Academic Institutions and Nonprofits only

  16. Start-Stop Assembly Toolkit

    Plasmid Kit
    #1000000152
    Purpose
    Start-Stop Assembly is a multi-part, modular DNA assembly method which is functionally scarless, suitable for combinatorial assembly, and ideal for metabolic engineering in diverse organisms.
    Depositor
    John Heap
    Available since
    March 20, 2019
    Availability
    Academic Institutions and Nonprofits only

  18. pMuSIC pool v1.0

    Pooled Library
    #227193
    Purpose
    Two-way barcodes from 18 fluorescent proteins for mammalian expression. Can be used for clone tracing, or for pairing with perturbations for single cell resolution in spectral detection measurements.
    Depositor
    Marc Birtwistle
    Article
    Lu et al bioRxiv [Preprint]. 2024 Oct 24:2024.10.
    Expression
    Mammalian
    Available since
    Dec. 18, 2024
    Availability
    Academic Institutions and Nonprofits only

  20. pAAV-hSyn-GRAB_OT1.0 (AAV1)

    Viral Prep
    #185386-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-hSyn-GRAB_OT1.0 (#185386). In addition to the viral particles, you will also receive purified pAAV-hSyn-GRAB_OT1.0 plasmid DNA. hSyn-driven expression of the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Yulong Li
    Article
    Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Promoter
    Syn
    Tags
    None
    Available since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only
Showing: 1461 - 1480 of 17137 results