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pmiRGLO Dual-Luciferase-PDAP1-3'UTR-Mut1x

Plasmid

#182253

Purpose

Human PDAP1 3` UTR region mutated in 1x binding site for miR-150 cloned downstream of luciferase reporter gene

Depositor

Silvia Monticelli

Article

Chirichella et al PLoS Biol. 2022 Feb 10;20(2):e3001538. d

Insert

3' UTR region of PDAP1 gene mutated in 1 binding site for mir-150

Use

Luciferase

Expression

Mammalian

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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pmiRGLO Dual-Luciferase-PDAP1-3'UTR-Mut3x

Plasmid

#182255

Purpose

Human PDAP1 3` UTR region mutated in 3x binding site for miR-150 cloned downstream of luciferase reporter gene

Depositor

Silvia Monticelli

Article

Chirichella et al PLoS Biol. 2022 Feb 10;20(2):e3001538. d

Insert

3' UTR region of PDAP1 gene mutated in 3 binding sites for mir-150

Use

Luciferase

Expression

Mammalian

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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pET28-His10-BTLAicd (AA190-289)-TwinStrep

Plasmid

#180805

Purpose

Expression of 10*His tagged human BTLA intracellular domain (AA190-289)-TwinStrep

Depositor

Enfu Hui

Article

Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

BTLAint (AA190-289)-TwinStrep (BTLA Human)

Expression

Bacterial

Promoter

T7

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-6p-2-GST-PD-1icd (AA194-288)

Plasmid

#180814

Purpose

Expression of GST fused human PD-1 intracellular domain (AA194-288)

Depositor

Enfu Hui

Article

Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

PD-1int (AA194-288) (PDCD1 Human)

Expression

Bacterial

Promoter

Tac

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pLX_HA-C7orf26v1

Plasmid

#178535

Purpose

Subcloned C7orf26v1 (ENST00000344417, Origene, RC219786L4) into pLX_HA backbone

Depositor

William Hahn

Article

Pan et al Cell Syst. 2022 Jan 21. pii: S2405-4712(

Insert

C7orf26v1 (INTS15 Human)

Tags

HA

Expression

Mammalian

Promoter

EF1a

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pSP72-RBM8A-3'UTR AluY-Sz mut 2598-4066

Plasmid

#175586

Purpose

in vitro transcription of mut RBM8A 3'UTR

Depositor

Emanuela Felley-Bosco

Article

Abukar et al Cells. 2021 Dec 15;10(12). pii: cells101

Insert

RBM8A-3'UTR AluY-Sz mut 2598-4066

Use

In vitro transcription

Promoter

T7

Available Since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pSIN-Gapdh

Plasmid

#175938

Purpose

knockout mouse Gapdh

Depositor

Dan Littman

Article

Wu et al Cell. 2020 Aug 6;182(3):641-654.e20. doi

Insert

Gapdh gRNA (Gapdh Mouse)

Use

Retroviral

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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MSCVpic2neo-EGFP-p16 shRNA

Plasmid

#164919

Purpose

Expresses EGFP cDNA and p16 shRNA in mammalian cells

Depositor

Ji Luo

Article

Smith et al Cell Biosci. 2016 Nov 11;6:57. doi: 10.1

Inserts

Enhanced green fluorescent protein
CDKN2A (CDKN2A Human)

Use

Retroviral

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA™5/FRT/CMV_promoter-ciCas9-E2A-mRFP

Plasmid

#164132

Purpose

Expresses ciCas9 and mRFP in mammalian cells (an insertion vector to generate ciCas9-knockin cell lines)

Depositor

Hyongbum Kim

Article

Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

Insert

ciCas9

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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miR290-SE-RGM-tdTomato targeting vector

Plasmid

#133047

Purpose

Targeting vector of RGM-tdTomato with homologous arms around Mir290 super-enhancer DMR

Depositor

Rudolf Jaenisch

Article

Song et al Mol Cell. 2019 Aug 15. pii: S1097-2765(1

Insert

miR290-SE-5arm-RGM-miR290-SE-3arm

Use

Mouse Targeting

Available Since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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