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pBHM2680

Plasmid

#241725

Purpose

Construction of fused marker-sgRNA inserts for Cryptococcus neoformans genome editing, contains BSR marker for selection on blasticidin S

Depositor

Hiten Madhani

Article

Boucher et al Microbiol Spectr. 2025 Sep 25:e0193525.

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available Since

Sept. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pTetR-BCD20-mCherry-ssrA P66U23C90T20 v40-3a (588)

Plasmid

#70745

Purpose

for building 3-color reporter plasmid

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

mCherry-ssrA

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pPUR-mU6-sgRNA-Sirius-8XPP7

Plasmid

#121943

Purpose

CRISPR-Sirius plasmid

Depositor

Thoru Pederson

Article

Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

Insert

sgRNA-Sirius-8XPP7

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Promoter

mouse U6 promoter

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-TO-dCas9-P2A-HSA

Plasmid

#121936

Purpose

CRISPR-Sirius plasmid

Depositor

Thoru Pederson

Article

Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

Insert

dCas9-P2A-HSA

Use

CRISPR and Lentiviral

Tags

HSA

Expression

Mammalian

Promoter

CMV-TO

Available Since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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HSA2_His

Plasmid

#202764

Purpose

human serum albumin design HSA2, in pET29b with C-terminal 6His-tag

Depositor

Sarel Fleishman

Article

Khersonsky et al J Mol Biol. 2023 Sep 1;435(17):168191. d

Insert

HSA2

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pC0090

Plasmid

#181954

Purpose

Beta catenin reporter M50 Super 8x (TCF/LEF binding sites) TOPFlash with Gluc/Cluc

Depositor

Feng Zhang

Article

Abudayyeh et al Science. 2019 Jul 11. pii: science.aax70

Insert

7xTOPFlash

Expression

Mammalian

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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pSV2 neuNT

Plasmid

#10918

Depositor

Bob Weinberg

Article

Bargmann et al Cell. 1986 Jun 6. 45(5):649-57.

Insert

neuNT (Erbb2 Rat)

Expression

Mammalian

Available Since

April 19, 2006

Availability

Academic Institutions and Nonprofits only
pCMV-G-Cdc42

Plasmid

#180577

Purpose

ddGFP sensor of Cdc42

Depositor

Won Do Heo

Article

Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

Insert

ddGFP sensor of Cdc42

Expression

Mammalian

Available Since

Feb. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pDHt/sk-PC

Plasmid

#92125

Purpose

Expression of codon-optimized Cas9 with Ppdc promoter in Trichoderma reesei

Depositor

Zhihua Zhou

Article

Liu et al Cell Discov. 2015 May 12;1:15007. doi: 1

Insert

Cas9

Use

CRISPR; Trichoderma reesei expression

Promoter

Ppdc

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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pDEST_TAF15

Plasmid

#26379

Depositor

Thomas Tuschl

Article

Hoell et al Nat Struct Mol Biol. 2011 Nov 13. doi: 1

Insert

TAF15 (TAF15 Human)

Tags

FLAG/HA

Expression

Mammalian

Available Since

Sept. 22, 2010

Availability

Academic Institutions and Nonprofits only