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EF1a_GATA6_P2A_Hygro_Barcode

Plasmid

#120445

Purpose

Barcoded lentiviral vector to express GATA6 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

GATA6 (GATA6 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pINDUCER21-SOX4

Plasmid

#51304

Purpose

Expression of SOX4 after doxycycline administration

Depositor

George Daley

Article

Doulatov et al Cell Stem Cell. 2013 Oct 3;13(4):459-70.

Insert

SRY (sex determining region Y)-box 4 (SOX4 Human)

Use

Lentiviral

Tags

n/a

Expression

Mutation

full length ORF

Promoter

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pQCXIP-BSR-GFP11

Plasmid

#68716

Purpose

Split GFP assay

Depositor

Yutaka Hata

Article

Kodaka et al Exp Cell Res. 2015 Aug 15;336(2):171-81.

Insert

bsr

Use

Retroviral

Tags

FLAG and GFP11

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pMXs-hs-LIN-41

Plasmid

#52716

Purpose

retroviral expression of human LIN-41 / TRIM71

Depositor

Shinya Yamanaka

Article

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

LIN-41 (TRIM71 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_Pmel-1_TCRB

Plasmid

#175288

Purpose

Expresses Pmel-1 TCR beta chain

Depositor

Jeff Hammerbacher

Article

Gottschalk et al bioRxiv 2021.11.30.470597

Insert

T cell receptor beta chain (Tcrb Mouse)

Use

Suitable for ivt

Tags

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_Pmel-1_TCRA

Plasmid

#175287

Purpose

Expresses Pmel-1 TCRA alpha chain

Depositor

Jeff Hammerbacher

Article

Gottschalk et al bioRxiv 2021.11.30.470597

Insert

T cell receptor alpha chain (Tcra Mouse)

Use

Suitable for ivt

Tags

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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EF1a_ESRRG_P2A_Hygro_Barcode

Plasmid

#120435

Purpose

Barcoded lentiviral vector to express ESRRG in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

ESRRG (ESRRG Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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AAVS1-Neo-CAG-Flpe-ERT2

Plasmid

#68460

Purpose

AAVS1 targeting donor plasmid with Flpe-ERT2 expression cassette and Neo selection gene

Depositor

Su-Chun Zhang

Article

Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

Insert

mammalian-codon-optimized Flpe recombinase fused with ERT2

Use

Synthetic Biology

Tags

ERT2

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Nov. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV5-Flag-HDAC3

Plasmid

#63676

Purpose

Mammalian expression of Flag tagged HDAC3

Depositor

Qingbo Xu, Lingfang Zeng

Article

Zeng et al J Cell Biol. 2006 Sep 25;174(7):1059-69.

Insert

HDAC3 (Hdac3 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 16, 2015

Availability

Academic Institutions and Nonprofits only
Hygro-iNGN3

Plasmid

#75340

Purpose

doxycycline inducible NGN3 gene over-expression

Depositor

Danwei Huangfu

Article

Zhu et al Cell Stem Cell. 2016 Apr 27. pii: S1934-

Insert

NGN3 (NEUROG3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

TRE-tight

Available since

June 16, 2016

Availability

Academic Institutions and Nonprofits only

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CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector

Plasmid

#159281

Purpose

A multicistronic vector with both CAGGS promoter-driven AsCpf1 and U6 promoter-driven single guide RNA (sgRNA)

Depositor

Rudolf Jaenisch

Article

Ma et al Cell Rep Med. 2020 Sep 22;1(6):100097. d

Insert

AsCpf1-HA-2A-GFP

Use

Multicistronic vector with both caggs promoter-dr…

Tags

3X HA

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 24, 2020

Availability

Academic Institutions and Nonprofits only

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SWEET11:SWEET11-2A-GFP-GUS

Plasmid

#172484

Purpose

Translational fusion of SWEET11 with a P2A self cleaving peptide sequence

Depositor

Wolf Frommer

Article

Kim et al Plant Cell. 2021 May 5;33(3):511-530. do

Insert

SWEET11 (AT3G48740 Mustard Weed)

Use

Tags

GFP, GUS

Expression

Plant

Mutation

Promoter

SWEET11

Available since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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EF1a_KLF16_P2A_Hygro_Barcode

Plasmid

#120502

Purpose

Barcoded lentiviral vector to express KLF16 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF16 (KLF16 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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LentiGuidPuro-hTP73_sgRNA-1

Plasmid

#88855

Purpose

CRISPR KO of Trp73

Depositor

Joan Massague

Article

Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

Insert

Trp73

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

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EF1a_LMX1A_P2A_Hygro_Barcode

Plasmid

#120457

Purpose

Barcoded lentiviral vector to express LMX1A in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

LMX1A (LMX1A Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_FOXA3_P2A_Hygro_Barcode

Plasmid

#120440

Purpose

Barcoded lentiviral vector to express FOXA3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

FOXA3 (FOXA3 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_FOXA1_P2A_Hygro_Barcode

Plasmid

#120438

Purpose

Barcoded lentiviral vector to express FOXA1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

FOXA1 (FOXA1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_CDX2_P2A_Hygro_Barcode

Plasmid

#120432

Purpose

Barcoded lentiviral vector to express CDX2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

CDX2 (CDX2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_MYC_P2A_Hygro_Barcode

Plasmid

#120461

Purpose

Barcoded lentiviral vector to express MYC in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

MYC (MYC Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_KLF1_P2A_Hygro_Barcode

Plasmid

#120488

Purpose

Barcoded lentiviral vector to express KLF1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF1 (KLF1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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