We narrowed to 1,541 results for: AdRed

1. pAP215.rAAV.mU6-sgRNA.Handle.EF1a-mTagBFP2

   Plasmid

   #217635

   Purpose

   AAV backbone for sgRNA expression. Contains a Lox-flanked handle sequence for Cre-dependent PCR amplification of sgRNAs. Protospacer is cloned between BstXI and BlpI.

   Depositor

   Martin Kampmann

   Article

   Ramani et al Nat Neurosci. 2025 Aug 22. doi: 10.1038/

   Insert

   mU6-sgRNA, Handle sequence, EF1a-mTagBFP2
   

   Use

   AAV

   Promoter

   EF1alpha and mU6

   Available Since

   Oct. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. CS2 Flag-Smad2 (3A)

   Plasmid

   #101763

   Purpose

   Expresses SMAD2 (3A) in mammalian cells

   Depositor

   Yuh-Shan Jou

   Article

   Yeh et al Nat Cell Biol. 2018 Apr;20(4):479-491. d

   Insert

   SMAD2 (SMAD2 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Three Ser to Ala mutations in the phosphorylation…

   Promoter

   CMV

   Available Since

   Oct. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR223_SMAD2_WT

   Plasmid

   #81830

   Purpose

   Gateway Donor vector containing SMAD2 ,part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, David Root, William Hahn

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   SMAD2 (SMAD2 Human)
   

   Use

   Gateway entry vector

   Mutation

   WT

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hSyn-DIO {hCAR}off-{ChETA-mRuby2}on-W3SL

   Plasmid

   #111391

   Purpose

   AAV vector with hSynapsin promoter, Cre-OFF hCAR (for efficient CAV-2 infection), Cre-ON ChETA-mRuby2 (for optogenetic activation), and W3SL regulatory cassette (for maximize cloning capacity)

   Depositor

   Adam Kepecs

   Article

   Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

   Inserts

   hCAR (CXADR Human)
   ChETA-mRuby2
   

   Use

   AAV

   Tags

   Myc and mRuby2

   Expression

   Mammalian

   Promoter

   hSynapsin

   Available Since

   June 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn-DIO {hCAR}off-{ChETA-EYFP}on-W3SL

   Plasmid

   #111392

   Purpose

   AAV vector with hSynapsin promoter, Cre-OFF hCAR (for efficient CAV-2 infection), Cre-ON ChETA-EYFP (for optogenetic activation), and W3SL regulatory cassette (for maximize cloning capacity)

   Depositor

   Adam Kepecs

   Article

   Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

   Inserts

   hCAR (CXADR Human)
   ChETA-EYFP
   

   Use

   AAV

   Tags

   EYFP and Myc

   Expression

   Mammalian

   Promoter

   hSynapsin

   Available Since

   June 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR223_SMAD2_p.D304G

   Plasmid

   #81615

   Purpose

   Gateway Donor vector containing SMAD2 , part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, David Root, William Hahn

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   SMAD2 (SMAD2 Human)
   

   Use

   Gateway entry vector

   Mutation

   D304G

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. dRED

   Plasmid

   #139836

   Purpose

   Expresses dRNase H1-EGFP-dCas9 fusion protein in human cells

   Depositor

   Karim Mekhail

   Article

   Abraham et al Nature. 2020 Sep;585(7824):298-302. doi:

   Insert

   dRNase H1-EGFP-dCas9 (RNASEH1 dRNase H1 (H. sapiens), dCas9 (S. pyogenes))
   

   Expression

   Mammalian

   Mutation

   RNase H1 protein contains D210N mutation. dCas9 …

   Promoter

   tet-inducible CMV

   Available Since

   Sept. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCR8 JARID2

   Plasmid

   #114443

   Purpose

   Entry vector for gateway cloning containing human JARID2 coding sequence

   Depositor

   Adrian Bracken

   Article

   Conway et al Mol Cell. 2018 May 3;70(3):408-421.e8. d

   Insert

   JARID2 (JARID2 Human)
   

   Use

   Gateway entry cloning

   Promoter

   None

   Available Since

   Sept. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. CRISPRoff-mScarletI

   Plasmid

   #217783

   Purpose

   Expresses CRISPRoff-mScarletI (DNMT3A-DNMT3L-XTEN80-dCas9-HA-2xNLS-mScarletI-KRAB) downstream of the CAG promoter for gene epigenetic silencing

   Depositor

   Adriano Aguzzi

   Article

   Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038

   Insert

   CRISPRoff-mScarletI (DNMT3A-DNMT3L-dCas9-mScarletI-KRAB)
   

   Tags

   2xNLS, DNMT3A-DNMT3L, HA, KRAB, and mScarletI

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCR8 PALI1

    Plasmid

    #114442

    Purpose

    Entry vector for gateway cloning containing human PALI1 coding sequence

    Depositor

    Adrian Bracken

    Article

    Conway et al Mol Cell. 2018 May 3;70(3):408-421.e8. d

    Insert

    PALI1 (LCOR Human)
    

    Use

    Gateway entry cloning

    Promoter

    None

    Available Since

    Sept. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCR8 LCOR

    Plasmid

    #114441

    Purpose

    Entry vector for gateway cloning containing human LCOR coding sequence

    Depositor

    Adrian Bracken

    Article

    Conway et al Mol Cell. 2018 May 3;70(3):408-421.e8. d

    Insert

    LCOR (LCOR Human)
    

    Use

    Gateway entry cloning

    Promoter

    None

    Available Since

    Sept. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pVL-FLAG/His PALI1

    Plasmid

    #114449

    Purpose

    Insect baculovirus expression vector containing N terminal FLAG/His tagged human PALI1 coding sequence

    Depositor

    Adrian Bracken

    Article

    Conway et al Mol Cell. 2018 May 3;70(3):408-421.e8. d

    Insert

    PALI1 (LCOR Human)
    

    Tags

    FLAG-His

    Expression

    Insect

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pD649-HAsp-IGSF11-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156652

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    IGSF11 (IGSF11 Human)
    

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pVL-FLAG/His LCOR

    Plasmid

    #114450

    Purpose

    Insect baculovirus expression vector containing N terminal FLAG/His tagged human LCOR coding sequence

    Depositor

    Adrian Bracken

    Article

    Conway et al Mol Cell. 2018 May 3;70(3):408-421.e8. d

    Insert

    LCOR (LCOR Human)
    

    Tags

    FLAG-His

    Expression

    Insect

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLENTI EF1alpha FLAG/HA-PALI1

    Plasmid

    #114445

    Purpose

    Mammalian lentiviral expression vector containing N terminal FLAG/HA tagged human PALI1 coding sequence

    Depositor

    Adrian Bracken

    Article

    Conway et al Mol Cell. 2018 May 3;70(3):408-421.e8. d

    Insert

    PALI1 (LCOR Human)
    

    Use

    Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLENTI EF1alpha FLAG/HA-LCOR

    Plasmid

    #114444

    Purpose

    Mammalian lentiviral expression vector containing N terminal FLAG/HA tagged human LCOR coding sequence

    Depositor

    Adrian Bracken

    Article

    Conway et al Mol Cell. 2018 May 3;70(3):408-421.e8. d

    Insert

    LCOR (LCOR Human)
    

    Use

    Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. Nsp3 Mac1 - C2 (UCSF)

    Plasmid

    #169209

    Purpose

    Expression of 6xHis tagged protein for crystallization, includes TEV protease site for tag removal

    Depositor

    James S Fraser

    Article

    Schuller et al Sci Adv. 2021 Apr 14;7(16). pii: 7/16/ea

    Insert

    SARS-CoV-2 Nsp3 Mac1
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 28, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. S1030

    Bacterial Strain

    #105063

    Purpose

    host strain for phage assisted continuous evolution

    Depositor

    David Liu

    Article

    Carlson et al Nat Chem Biol. 2014 Mar;10(3):216-22. do

    Bacterial Resistance

    Streptomycin and tetracycline

    Available Since

    March 21, 2018

    Availability

    Academic Institutions and Nonprofits only

19. Nsp3 Mac1 - P43 (Oxford)

    Plasmid

    #169210

    Purpose

    Expression of 6xHis tagged protein for crystallization, includes TEV protease site for tag removal

    Depositor

    James S Fraser

    Article

    Schuller et al Sci Adv. 2021 Apr 14;7(16). pii: 7/16/ea

    Insert

    SARS-CoV-2 Nsp3 Mac1
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 28, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pEMS1167

    Plasmid

    #29082

    Purpose

    Expression of the eGFP reporter gene was not detected in the brain and eye.

    Depositor

    Elizabeth Simpson

    Article

    Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

    Insert

    Ple62 (DRD1 Human)
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    EGFP-NLS

    Available Since

    Feb. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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