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We narrowed to 173,443 results for: addgene

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Showing: 1501 - 1520 of 173443 results

  1. p14-9VN9-3A

    Plasmid
    #50932
    Purpose
    Expresses human SRP14 and SRP9-3A proteins as a fusion protein in mammalian cells.
    Depositor
    Katharina Strub
    Insert
    SRP14 and SRP9 (SRP14, SRP9 Human)
    Tags
    VN tag and flag tag
    Expression
    Mammalian
    Mutation
    Amino acids 60, 61 and 64 of SRP9 changed to alan…
    Promoter
    CMV
    Available Since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  3. pJF89

    Plasmid
    #253756
    Purpose
    Encodes GFP_AID* and pRNR2-OsTIR1(F74G, S210A) expression cassettes. The OsTIR1(F74G, S210A) coding sequence is codon optimized for S. cerevisiae.
    Depositor
    Matthias Heinemann
    Inserts
    GFP_AID*
    OsTIR1
    Tags
    mAID*-tag
    Expression
    Yeast
    Mutation
    F74G, S210A
    Promoter
    RNR2 and TEF1
    Available Since
    May 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    253756-plasmid-map-sequence-id-509520

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  4. pJF90

    Plasmid
    #253757
    Purpose
    Encodes GFP_AID* and pREV1-OsTIR1(F74G, S210A) expression cassettes. The OsTIR1(F74G, S210A) coding sequence is codon optimized for S. cerevisiae.
    Depositor
    Matthias Heinemann
    Inserts
    GFP_AID*
    OsTIR1
    Tags
    mAID*-tag
    Expression
    Yeast
    Mutation
    F74G, S210A
    Promoter
    REV1 and TEF1
    Available Since
    May 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    253757-plasmid-map-sequence-id-509519

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  5. pJF91

    Plasmid
    #253758
    Purpose
    Encodes GFP_AID* and pADH1-OsTIR1(F74G, S210A) expression cassettes. The OsTIR1(F74G, S210A) coding sequence is codon optimized for S. cerevisiae.
    Depositor
    Matthias Heinemann
    Inserts
    GFP_AID*
    OsTIR1
    Tags
    mAID*-tag
    Expression
    Yeast
    Mutation
    F74G, S210A
    Promoter
    ADH1 and TEF1
    Available Since
    May 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    253758-plasmid-map-sequence-id-509518

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  6. pJF86

    Plasmid
    #253753
    Purpose
    Encodes GFP_AID* and pRPL18B-OsTIR1(F74G, S210A) expression cassettes. The OsTIR1(F74G, S210A) coding sequence is codon optimized for S. cerevisiae.
    Depositor
    Matthias Heinemann
    Inserts
    GFP_AID*
    OsTIR1
    Tags
    mAID*-tag
    Expression
    Yeast
    Mutation
    F74G, S210A
    Promoter
    RPL18B and TEF1
    Available Since
    May 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    253753-plasmid-map-sequence-id-509401

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  7. pJF88

    Plasmid
    #253755
    Purpose
    Encodes GFP_AID* and pSAC6-OsTIR1(F74G, S210A) expression cassettes. The OsTIR1(F74G, S210A) coding sequence is codon optimized for S. cerevisiae.
    Depositor
    Matthias Heinemann
    Inserts
    GFP_AID*
    OsTIR1
    Tags
    mAID*-tag
    Expression
    Yeast
    Mutation
    F74G, S210A
    Promoter
    SAC6 and TEF1
    Available Since
    May 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    253755-plasmid-map-sequence-id-509369

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  8. pJF87

    Plasmid
    #253754
    Purpose
    Encodes GFP_AID* and pALD6-OsTIR1(F74G, S210A) expression cassettes. The OsTIR1(F74G, S210A) coding sequence is codon optimized for S. cerevisiae.
    Depositor
    Matthias Heinemann
    Inserts
    GFP_AID*
    OsTIR1
    Tags
    mAID*-tag
    Expression
    Yeast
    Mutation
    F74G, S210A
    Promoter
    ALD6 and TEF1
    Available Since
    April 27, 2026
    Availability
    Academic Institutions and Nonprofits only
    253754-plasmid-map-sequence-id-507832

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  9. DN-28z-CAR-miSFIT-Scramble

    Plasmid
    #221640
    Purpose
    Control for tuning expression of DN-28z CAR
    Depositor
    Tatjana Sauka-Spengler
    Insert
    DN-28z chimeric antigen receptor (CAR)
    Use
    Affinity Reagent/ Antibody
    Expression
    Mammalian
    Available Since
    March 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    221640-plasmid-map-sequence-id-504454

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  10. pLexM-MmKDELR3-FLAG

    Plasmid
    #250430
    Purpose
    Expression of C-terminally FLAG-Tagged Mouse KDELR3 in Mammalian Cells
    Depositor
    Simon Newstead
    Insert
    KDELR3 (Kdelr3 Mouse)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    WT (Additional C-terminal Glycine)
    Promoter
    Chicken-?-actin promoter/CMV Enhancer
    Available Since
    Feb. 6, 2026
    Availability
    Academic Institutions and Nonprofits only
    250430-plasmid-map-sequence-id-499045

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  11. pLFYa

    Plasmid
    #243803
    Purpose
    Dropout vector for genomic integration into yeast strain LFYa. GFP is flanked by BamHI sites for easy cloning using Gibson assembly. Construct contains the attP site for the serine recombinase BxB1.
    Depositor
    Sai Reddy
    Article
    Lester et al bioRxiv
    Type
    Empty backbone
    Use
    Synthetic Biology
    Available Since
    Oct. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    243803-plasmid-map-sequence-id-487915

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  12. pLFYalpha

    Plasmid
    #243804
    Purpose
    Dropout vector for genomic integration into yeast strain LFYalpha. GFP is flanked by BamHI sites for easy cloning using Gibson assembly. Construct contains attB site for the serine recombinase BxB1.
    Depositor
    Sai Reddy
    Article
    Lester et al bioRxiv
    Type
    Empty backbone
    Use
    Synthetic Biology
    Available Since
    Oct. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    243804-plasmid-map-sequence-id-487916

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  13. (915) SB EF1a dCasphi-[myc-ZIM3-HA] U6-gStop

    Plasmid
    #195623
    Purpose
    Sleeping Beauty EF1a plasmid for constitutive expression of dCasphi-ZIM3 CRISPRi construct
    Depositor
    Tonia Rex
    Insert
    dCasphi
    Tags
    C-Myc (NLS), HA tag, and ZIM3
    Expression
    Mammalian
    Mutation
    P749A and P753A
    Promoter
    EF1a (Mlu1/EcoR1)
    Available Since
    July 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    195623-plasmid-map-sequence-id-385117

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  14. LentiCRISPRv2-ACTB-C4

    Plasmid
    #229849
    Purpose
    Expresses wild-type Cas9 and gRNA for ACTB gene. The target sequence is changed from ACTB-C1 to improve the cut efficiency.
    Depositor
    Natsuko Chiba
    Insert
    guide RNA for ACTB gene
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    229849-plasmid-map-sequence-id-462154

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  15. pX330-U6-MYC-Guide-hSpCas9

    Plasmid
    #228574
    Purpose
    Expression of human MYC gRNA; CRISPR mediated gene insertion
    Depositor
    Marcin Imielinski
    Insert
    MYC gRNA (MYC Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Jan. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    228574-plasmid-map-sequence-id-457356

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  16. pCW57.1-GFP_mmu-miR-292b-3p_sponge

    Plasmid
    #132622
    Purpose
    Expresses a TurboGFP transcript with test microRNA sponge for mmu-miR-292b-3p as 3'UTR; serves as template for other microRNA sponges
    Depositor
    Constance Ciaudo
    Insert
    stop codon, mmu-miR-292b-3p sponge sequence and polyA signal
    Use
    Lentiviral; Doxycycline inducible
    Expression
    Mammalian
    Promoter
    TRE
    Available Since
    Aug. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    132622-plasmid-map-sequence-id-259572

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  17. pDK362

    Plasmid
    #134194
    Purpose
    Retroviral plasmid with SKAP insert
    Depositor
    Iain Cheeseman
    Insert
    SKAP 166-316-LAP with G5 and Jens siRNA hardening (KNSTRN Human)
    Use
    Retroviral
    Tags
    LAP (GFP)
    Expression
    Mammalian
    Mutation
    aa 166-316 with G5 and Jens siRNA hardening
    Available Since
    Feb. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    134194-plasmid-map-sequence-id-272444

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  18. pBRCA2.1.0-gDNA

    Plasmid
    #132448
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    BRCA2 (BRCA2 Human)
    Use
    CRISPR
    Available Since
    Dec. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    132448-plasmid-map-sequence-id-266805

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  19. pKLF6.1.0-gDNA

    Plasmid
    #132435
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    KLF6 (KLF6 Human)
    Use
    CRISPR
    Available Since
    Dec. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    132435-plasmid-map-sequence-id-266217

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  20. pZNF462.1.0-gDNA

    Plasmid
    #112476
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF462
    Depositor
    Kevin White
    Insert
    ZNF462 (ZNF462 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112476-plasmid-map-sequence-id-230510

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Showing: 1501 - 1520 of 173443 results