We narrowed to 7,759 results for: plasmid dna

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pcDNA3.3_c-MYC

Plasmid

#26818

Depositor

Derrick Rossi

Article

Warren et al Cell Stem Cell. 2010 Nov 5;7(5):618-30.

Insert

5'UTR-c-MYC-3'UTR (MYC Human)

Expression

Mammalian

Mutation

Sequence begins from second ATG; Synthetic UTR se…

Available Since

Nov. 17, 2010

Availability

Industry, Academic Institutions, and Nonprofits

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GFP-pcDNA3-PKCgamma-cys1Acys1B

Plasmid

#21190

Depositor

Tobias Meyer

Article

Oancea et al J Cell Biol. 1998 Feb 9. 140(3):485-98.

Insert

PKCgamma-cys1Acys1B (Prkcg Rat)

Tags

GFP

Expression

Mammalian

Mutation

PKCgamma-cys1Acys1B (aa35 to aa152 of rat PKCgamm…

Available Since

July 15, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 - HA-KLF4 FL delta K5S

Plasmid

#34594

Depositor

Michael Ruppert

Article

Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

Insert

KLF4 (KLF4 Human)

Tags

HA tag

Expression

Mammalian

Mutation

deletion of K5S, corresponding to bases 1781 to 2…

Promoter

CMV

Available Since

Jan. 24, 2012

Availability

Academic Institutions and Nonprofits only
CelTag Plasmid

Plasmid

#66562

Purpose

Encodes CelTag for the tagging and high affinity purification of endogenous yeast proteins.

Depositor

David Engelke

Article

Carrick et al G3 (Bethesda). 2015 Dec 29. pii: g3.115.

Insert

CelTag

Expression

Bacterial

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pET28_ancCas9d_Plasmid

Plasmid

#239444

Purpose

Expression plasmid codon-optimized for E. coli

Depositor

David Taylor

Article

Ocampo et al Nat Commun. 2025 Jan 7;16(1):457. doi: 1

Insert

ancCas9d

Expression

Bacterial

Promoter

T7

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pET28_Cas9d_Plasmid

Plasmid

#239445

Purpose

Expression plasmid codon-optimized for E. coli

Depositor

David Taylor

Article

Ocampo et al Nat Commun. 2025 Jan 7;16(1):457. doi: 1

Insert

Cas9d

Expression

Bacterial

Promoter

T7

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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CJ8421_04975 Rptr2 recording plasmid

Plasmid

#194211

Purpose

CJ8421_04975 Rptr2 targeted DNA sequence

Depositor

Chase Beisel

Article

Jiao et al Nat Biotechnol. 2023 Jan 5. doi: 10.1038

Insert

CJ8421_04975 Rptr2 DNA target

Expression

Bacterial

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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T7 promoter + rpsL reporter plasmid

Plasmid

#156455

Purpose

A mutational reporter plasmid that can gain Streptomycin and/or Tetracycline resistance upon mutation. Contains T7 promoter.

Depositor

Matthew D. Shoulders

Article

Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

Inserts

rpsL
TcR

Expression

Bacterial

Mutation

ATG start codon mutated to ACG

Promoter

tac and tet

Available Since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pUC19-SpRYgest_targetplasmid (KAC833)

Plasmid

#181748

Purpose

pUC19 derivative plasmid used as a substrate for SpRYgest, restriction enzyme, and TtAgo digests. Encodes an SpCas9 EMX1-site1-NGG-PAM target site between NheI and HindIII restriction sites.

Depositor

Benjamin Kleinstiver

Article

Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

Type

Empty backbone

Use

Substrate plasmid

Available Since

Feb. 16, 2022

Availability

Academic Institutions and Nonprofits only

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T7 promoter + terminators reporter plasmid

Plasmid

#156456

Purpose

A mutational reporter plasmid that can gain Kanamycin and/or Tetracycline resistance upon mutation. Contains T7 promoter and a terminator array.

Depositor

Matthew D. Shoulders

Article

Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

Inserts

NeoR/KanR
TcR

Expression

Bacterial

Mutation

ATG start codon mutated to ACG

Promoter

Em7 and tet

Available Since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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Dual opposing T7 promoters reporter plasmid

Plasmid

#156457

Purpose

A mutational reporter plasmid that can gain Kanamycin resistance upon mutation. Contains two T7 promoters flanking the kanamycin resistance gene.

Depositor

Matthew D. Shoulders

Article

Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

Insert

NeoR/KanR

Expression

Bacterial

Mutation

ATG start codon mutated to ACG

Promoter

Em7

Available Since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pSL3927 (pDonor(plasmid)_Pse)

Plasmid

#200905

Purpose

Encodes a mini-transposon derived from Pseudoalteromonas Tn7016,with a CmrR cargo, as well as a primer binding site for deep sequencing purposes at pTarget. Total transposon size = 1,087 bp.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

Mini Tn7016 (pTarget NGS)

Expression

Mammalian

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pSHS306 - Bacterial expression plasmid for SpCas9, HNH FRET variant

Plasmid

#87350

Purpose

S867C/S355C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, HNH-1 FRET construct

Depositor

Jennifer Doudna

Article

Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

Insert

Cas9

Tags

His-MBP

Expression

Bacterial

Mutation

C80S/S355C/C574S/S867C

Promoter

T7

Available Since

March 22, 2017

Availability

Academic Institutions and Nonprofits only

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Endy/Shearwin pOSIP plasmid set

Plasmid Kit

#1000000035

Purpose

11 Plasmids for use in clonetegration: method for decreasing time and effort needed for integration of DNA into prokaryotic chromosomes. Facilitates cloning, expression of difficult genetic elements

Depositor

Drew Endy

Available Since

July 26, 2013

Availability

Academic Institutions and Nonprofits only
Lentiviral click editor (CE1) plasmid - LTR-pEF1a-PCV2-nCas9-EcKlenow (DRR921)

Plasmid

#217798

Purpose

Lentiviral plasmid to produce the prototypical CE1 construct (PCV2-nCas9-EcKlenow), expressed from the EF1a promoter

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS

Use

CRISPR and Lentiviral

Tags

BPNLS

Expression

Mammalian

Mutation

nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

Promoter

EF1a

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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dCas9 plasmid

Plasmid

#100091

Purpose

dCas9 expression plasmid without effector fusions; 3X Flag tag; 2NLS; pCDNA3 vector backbone, mammalian expression

Depositor

David Segal

Article

O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

Type

Empty backbone

Use

CRISPR

Tags

3XFlag-NLS-dCas9-NLS

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pU6-hL1-5'_3.3kb plasmid

Plasmid

#226006

Purpose

Plasmid used to generate DNA-FISH probe targeting at human L1HS

Depositor

Miguel Ramalho-Santos

Article

Zhang et al Dev Cell. 2024 Oct 14:S1534-5807(24)0057

Insert

Human L1 element 3.3kb sequences at the 5' end

Use

Synthetic Biology

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pSHS207 - Bacterial expression plasmid for WT SpCas9

Plasmid

#101199

Purpose

Bacterial expression plasmid for WT SpCas9

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Promoter

T7

Available Since

Nov. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pJRH-1362 scFv entry plasmid

Plasmid

#201912

Purpose

Entry vector for cloning single chain variable fragments displayed on the human CD8a hinge and transmembrane domain (CAG promoter)

Depositor

Jennifer Doudna

Article

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Insert

Stuffer sequence (drop out for scFv cloning) + CD8 hinge and transmembrane domain

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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UBC plasmid

Plasmid

#169746

Purpose

Unregulated "open-loop" UBC promoter plasmid. This plasmid encodes eGFP to report circuit output levels.

Depositor

Francois St-Pierre

Article

Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

Insert

EGFP

Use

Synthetic Biology

Expression

Mammalian

Promoter

UBC

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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