We narrowed to 7,548 results for: ski

1. pUC57-attB2-SA(0)-CRY2-mCherry

   Plasmid

   #160439

   Purpose

   To create Cry2-mCherry lines from MiMIC lines with inserts into coding introns in Phase 0.

   Depositor

   Nicholas Tolwinski

   Article

   Bunnag et al J Mol Biol. 2020 May 1;432(10):3159-3176

   Insert

   Cryptochrome 2 (CRY2 Mustard Weed)
   

   Use

   Tags

   mCherry

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCellFree_G03 AKT1

   Plasmid

   #67102

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   AKT1 (AKT1 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pCellFree_G03 BMPR1A

   Plasmid

   #67105

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   BMPR1A (BMPR1A Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pCellFree_G03 EHD2

   Plasmid

   #67107

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   EHD2 (EHD2 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   S320T, E384D, T387A, H388Q, T405V, V407A, H418R, …

   Promoter

   T7

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pCellFree_G03 GTF2H1

   Plasmid

   #67108

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   GTF2H1 (GTF2H1 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pRevLCav2FeS

   Plasmid

   #60808

   Purpose

   to disrupt iron sulfur cluster in yeast Rev3

   Depositor

   Youri Pavlov, Tahir Tahirov

   Article

   Baranovskiy et al J Biol Chem. 2012 May 18;287(21):17281-7

   Insert

   REV3 (REV3 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   contains 647 C-terminal amino acids and 335 bp of…

   Promoter

   Available since

   March 9, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pBABEpuro-ARAF_D429A

   Plasmid

   #51080

   Purpose

   mammalian expression of ARAF D429A

   Depositor

   Matthew Meyerson

   Article

   Imielinski et al J Clin Invest. 2014 Apr;124(4):1582-6. d

   Insert

   ARAF (ARAF Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   D429A

   Promoter

   Available since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJK048

   Plasmid

   #75230

   Purpose

   Produces Escherichia coli N5-carboxyaminoimidazole ribonucleotide mutase (EcPurE1-H45W) and N5-carboxyaminoimidazole ribonucleotide synthetase (EcPurK)

   Depositor

   T. J. Kappock, JoAnne Stubbe

   Article

   Hoskins et al Biochemistry. 2007 Mar 13;46(10):2842-55

   Inserts

   N5-carboxyaminoimidazole ribonucleotide mutase
   N5-carboxyaminoimidazole ribonucleotide synthetase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Pro64 to Arg; Asp65 to His; Gln206 to Arg and cha…

   Promoter

   T7 and n/a

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLEX-Frb:Akt2-IRES-Hygromycin

   Plasmid

   #120713

   Purpose

   Expresses Frb:Akt2 fusion protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   AKT2 (AKT2 Human)
   

   Use

   Lentiviral

   Tags

   FRB(T2098L)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFUSE-CHIg-hG1_AT118-L-Fc LALA + PG

    Plasmid

    #222852

    Purpose

    Mammalian expression plasmid for secretion of AT118-L a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R) fused to human IgG1 Fc

    Depositor

    Andrew Kruse

    Article

    Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

    Insert

    AT118-L
    

    Use

    Tags

    IgG1_Fc

    Expression

    Mammalian

    Mutation

    L234A, L235A, P329G

    Promoter

    Available since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMT1-24X-NOG(AtUBI10)

    Plasmid

    #202016

    Purpose

    T-DNA vector for expression of the two protein components of the MoonTag system (dCas9-24XGP41 and NbGP41P-sfGFP-VP64-GB1) under the control of the AtUBI10 promoter.

    Depositor

    Michael Smanski

    Article

    Casas-Mollano et al Nucleic Acids Research, Volume 51, Issue

    Inserts

    dCas9-24XGP41
    NbGP41P-sfGFP-VP64-GB1
    

    Use

    Tags

    GB1 and sfGFP

    Expression

    Plant

    Mutation

    Promoter

    AtUBI10

    Available since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. Mfn2-YFP

    Plasmid

    #28010

    Depositor

    Richard Youle

    Article

    Karbowski et al J Cell Biol. 2002 Dec 23. 159(6):931-8.

    Insert

    mitofusin 2 (MFN2 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    silent S508S

    Promoter

    Available since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFUSE-CHIg-hG1_AT118-L F47T, Y98F-Fc LALA + PG

    Plasmid

    #222854

    Purpose

    Mammalian expression plasmid for secretion of a non-AT1R binding control AT118-L variant fused to human IgG1 Fc

    Depositor

    Andrew Kruse

    Article

    Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

    Insert

    AT118-L
    

    Use

    Tags

    IgG1_Fc

    Expression

    Mammalian

    Mutation

    L234A, L235A, P329G

    Promoter

    Available since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. PB-CAG-DDdCas9VPH-T2A-GFP-IRES-Neo

    Plasmid

    #102886

    Purpose

    PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    DDdCas9VP192-p65-HSF1-T2A-GFP-IRES-Neo
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    CAG

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. A*66:01:01

    Plasmid

    #203291

    Purpose

    Overexpress HLA allele

    Depositor

    Derin Keskin, Catherine Wu

    Article

    Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

    Insert

    A*66:01:01 (HLA-A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDsRed1-CMV-Rat Arc-DsRed1-pA

    Plasmid

    #233049

    Purpose

    To Express a Rat Arc-DsRed fusion protein from a CMV promoter

    Depositor

    Jonathan Ploski

    Article

    de Solis et al Neurobiol Learn Mem. 2015 Oct;124:34-47.

    Insert

    rat Arc-DsRed fusion (Arc Rat)
    

    Use

    Tags

    DsRed1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. A*11:01:01:01

    Plasmid

    #203276

    Purpose

    Overexpress HLA allele

    Depositor

    Derin Keskin, Catherine Wu

    Article

    Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

    Insert

    A*11:01:01:01 (HLA-A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEF5/FRT/DEST-3xHA/mGli2

    Plasmid

    #51250

    Purpose

    Encodes N-3xHA-TEV-mouseGli2-C

    Depositor

    Rajat Rohatgi

    Article

    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

    Insert

    Gli2 (Gli2 Mouse)
    

    Use

    Flpin system

    Tags

    3xHA-TEV

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pLKO.1P CISD1_1

    Plasmid

    #160773

    Purpose

    Suppress CISD1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shCISD1_1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLKO.1P CISD1_2

    Plasmid

    #160774

    Purpose

    Suppress CISD1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shCISD1_2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge